Elemental mapping of biofortified wheat grains using micro X-ray fluorescence

Ramos, Inês N.; Pataco, Inês Maria; Mourinho, Mara Palma; Lidon, Fernando C.; Reboredo, Fernando H.; Pessoa, Maria Fernanda; Carvalho, Luísa; Santos, J. P.; Guerra, Mauro

doi:10.1016/j.sab.2016.03.014

Cited by 35 publications

(19 citation statements)

References 16 publications

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“…In contrast, some barley genotypes showed higher accumulation of Zn in the endosperm where it can create a complex with S ligands (Detterbeck et al , ). The N and S nutrition levels have shown strong effects on the deposition and spatial distribution of Zn in the endosperm of wheat grain (Persson et al , ); similar results were obtained for biofortified wheat grains under different nutrient concentrations (Ramos et al , ).…”

Section: Discussionmentioning

confidence: 55%

Variation in phosphorus and sulfur content shapes the genetic architecture and phenotypic associations within the wheat grain ionome

et al. 2019

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Dissection of the genetic basis of wheat ionome is crucial for understanding the physiological and biochemical processes underlying mineral accumulation in seeds, as well as for efficient crop breeding. Most of the elements essential for plants are metals stored in seeds as chelate complexes with phytic acid or sulfur-containing compounds. We assume that the involvement of phosphorus and sulfur in metal chelation is the reason for strong phenotypic correlations within ionome. Adjustment of element concentrations for the effect of variation in phosphorus and sulfur seed content resulted in drastic change of phenotypic correlations between the elements. The genetic architecture of wheat grain ionome was characterized by quantitative trait loci (QTL) analysis using a cross between durum and wild emmer wheat. QTL analysis of the adjusted traits and two-trait analysis of the initial traits paired with either P or S considerably improved QTL detection power and accuracy, resulting in the identification of 105 QTLs and 617 QTL effects for 11 elements. Candidate gene search revealed some potential functional associations between QTLs and corresponding genes within their intervals. Thus, we have shown that accounting for variation in P and S is crucial for understanding of the physiological and genetic regulation of mineral composition of wheat grain ionome and can be implemented for other plants.

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Section: Discussionmentioning

confidence: 55%

Variation in phosphorus and sulfur content shapes the genetic architecture and phenotypic associations within the wheat grain ionome

et al. 2019

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“…The X-ray technique is based on the absorption of these rays by the tissues, which are transposed to the image (radiography) in gray shades. In addition to being non-destructive, this technique has been efficient in identification of internal seed characteristics (RAHMAN;CHO, 2016), such as nutrient accumulation (CAPPA et al, 2015;RAMOS et al, 2016); modifications of embryo reserves (NIELSEN; DAMKJAER; FEIDENHANS'L, 2017), percentage of the space occupied in the internal cavity (NORONHA; MEDEIROS; PEREIRA, 2018) and physical damage that directly reflect on seed germination and vigor (ABUD; CICERO; .…”

Section: Introductionmentioning

confidence: 99%

Non-destructive identification of physical damage in mung bean (Vigna radiata L.) seeds by X-ray image analysis

et al. 2020

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Global demand for pulses such as the mung bean has grown in the last years. For successful production of these crops it is necessary to use high quality seeds. Methodologies based on X-ray image analysis have been used as a complementary tool to evaluate the physical quality of seeds due to their speed and potential for automation. The aim of this study was to evaluate the efficiency of X-ray analysis for non-destructive evaluation of the physical quality of Vigna radiata seeds and to relate the variables obtained with their physiological potential. For this, seeds from eight lots were X-rayed and subsequently subject to germination test. In total, 18 physical and physiological parameters were determined. The X-ray image analysis was efficient for evaluating the internal morphology of Vigna radiata seeds and allowed the identification of various damage types. However, it was not possible to relate the physical variables to the seed quality as the lots presented similar germination percentage. Physical variables such as solidity and circularity are related to percentage of root protrusion and length of seedling hypocotyl. Low relative densities indicate deteriorated tissues, related to severe morphological damage and non-viable seeds.

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“…Micro X-ray fluorescence evaluation of mineral localization of nutrients in wheat grain was determined using a µ-Energy Dispersive X-ray Fluorescence system (M4 Tornado™, Bruker, Germany), as previously optimized (Ramos et al 2016). Briefly, the X-ray generator was operated at 50 kV and 100 µA without the use of filters, to enhance the ionization of low-Z elements.…”

Section: Nutrients Depositionmentioning

confidence: 99%

Biofortification of durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) grains with nutrients

Pataco

Lidon

Ramos

et al. 2017

Journal of Plant Interactions

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Durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) was grown under conditions to promote mineral biofortification at the grain level. Along plant development, biomass accumulation and the kinetics of nutrients accumulation were assessed, identifying the nutrient fluxes of roots and shoots, and the timescale constraints of crop biofortification. Plants were grown under environmentally controlled conditions, submitted to four increasing concentrations of nutrient solutions (1-, 2-, 4-and 6-fold) of micro-(Fe, Zn, Cu and Mn) and macronutrients (Ca, K, P and Mg). The threshold of mineral toxicity was not reached as evaluated through plant biomass accumulation, but considering grain yield, the twofold nutrient concentration was the best treatment for biofortification. In the different treatments, the contents and the mineral unrests of roots uptake and shoots translocation varied, at different magnitudes and trends, before the onset of booting and from the physiological maturity onwards. Except for Cu, all mineral nutrients were mainly detected in the bran and embryo of the grains; therefore, the production of biofortified pasta for human consumption requires the use of integral semolina. ARTICLE HISTORY

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Elemental mapping of biofortified wheat grains using micro X-ray fluorescence

Cited by 35 publications

References 16 publications

Variation in phosphorus and sulfur content shapes the genetic architecture and phenotypic associations within the wheat grain ionome

Variation in phosphorus and sulfur content shapes the genetic architecture and phenotypic associations within the wheat grain ionome

Non-destructive identification of physical damage in mung bean (Vigna radiata L.) seeds by X-ray image analysis

Biofortification of durum wheat (Triticum turgidum L. ssp. durum (Desf.) Husnot) grains with nutrients

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