Elevated chaperone proteins are a feature of winter freeze avoidance by larvae of the goldenrod gall moth, Epiblema scudderiana

Zhang, Guijun; Storey, Janet M.; Storey, Kenneth B.

doi:10.1016/j.jinsphys.2017.04.007

Cited by 11 publications

(9 citation statements)

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“…Significant SNPs were identified in or close to three genes encoding putative chaperone proteins. Regulation of mitochondrial chaperone proteins has been identified in association with temperature adaptation in diverse organisms such as the moth Epiblema scudderiana and the fungus S. cerevisiae (Schmitt, 1996 ; Lyons et al, 2015 ; Zhang et al, 2018 ). As chaperone proteins have also been discussed as important for cold tolerance in plants as well, this result demonstrates an interesting basis for further studies of chaperone importance in cold stress in C. rosea (Al-Whaibi, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Out in the Cold: Identification of Genomic Regions Associated With Cold Tolerance in the Biocontrol Fungus Clonostachys rosea Through Genome-Wide Association Mapping

et al. 2018

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There is an increasing importance for using biocontrol agents in combating plant diseases sustainably and in the long term. As large scale genomic sequencing becomes economically viable, the impact of single nucleotide polymorphisms (SNPs) on biocontrol-associated phenotypes can be easily studied across entire genomes of fungal populations. Here, we improved a previously reported genome assembly of the biocontrol fungus Clonostachys rosea strain IK726 using the PacBio sequencing platform, which resulted in a total genome size of 70.7 Mbp and 21,246 predicted genes. We further performed whole-genome re-sequencing of 52 additional C. rosea strains isolated globally using Illumina sequencing technology, in order to perform genome-wide association studies in conditions relevant for biocontrol activity. One such condition is the ability to grow at lower temperatures commonly encountered in cryic or frigid soils in temperate regions, as these will be prevalent for protecting growing crops in temperate climates. Growth rates at 10°C on potato dextrose agar of the 53 sequenced strains of C. rosea were measured and ranged between 0.066 and 0.413 mm/day. Performing a genome wide association study, a total of 1,478 SNP markers were significantly associated with the trait and located in 227 scaffolds, within or close to (< 1000 bp distance) 265 different genes. The predicted gene products included several chaperone proteins, membrane transporters, lipases, and proteins involved in chitin metabolism with possible roles in cold tolerance. The data reported in this study provides a foundation for future investigations into the genetic basis for cold tolerance in fungi, with important implications for biocontrol.

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Section: Discussionmentioning

confidence: 99%

Out in the Cold: Identification of Genomic Regions Associated With Cold Tolerance in the Biocontrol Fungus Clonostachys rosea Through Genome-Wide Association Mapping

et al. 2018

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“…Accumulating sufficient lipids is essential for insects to successfully complete diapause 37–40 . sHSPs assist in ensuring the correct folding of nascent proteins and help to refold stress‐accumulated misfolded proteins, they may affect insect development and stress tolerance 41–45 . Hence, the gene functions of FAS, HSP19.8, and HSP18.9 were further validated by RNAi.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic and proteomic analyses of the mechanisms of overwintering diapause in soybean pod borer (Leguminivora glycinivorella)

Yang

Wang

et al. 2020

Pest Management Science

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BACKGROUND: Soybean pod borer (Leguminivora glycinivorella) is an important soybean pest in northeastern Asia, whose mature larvae overwinter in a diapause state. Disruption of winter diapause may be a valuable tool in pest management. However, the molecular mechanisms regulating diapause in this species have not yet been elucidated. RESULTS: We compared the transcriptomes and proteomes between diapause and mature larvae and between mature and newly developed pupae to identify the genes and proteins associated with diapause. Thirty-seven differentially expressed genes and their proteins changed synchronously between diapause and mature larvae and 82 changed synchronously between diapause larvae and newly developed pupae. Among these, genes involved in fatty acid biosynthesis and the longevity regulating pathway were up-regulated in diapause larvae and down-regulated in newly developed pupae, suggesting that they may regulate diapause. One fatty acid synthase (FAS) gene and two small heat shock genes (HSP19.8 and HSP18.9) were chosen for further functional analysis. After RNA interference (RNAi)-mediated knockdown of FAS, the survival of mature larvae was significantly lower than that of control larvae, but the mean developmental time from first-instar larva to adult remained unchanged. RNAi-mediated knockdown of HSP19.8 and HSP18.9 severely shortened the mean developmental time, causing approximately 50% larvae to develop directly into pupae. CONCLUSION: FAS and the small heat shock gene play roles in diapause regulation and larvae survival. This study provides important information that may assist in understanding the molecular regulatory mechanisms of overwintering diapause of this important agricultural insect pest.

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“…DAPI, DAPI staining; FITC, antibody staining; scale bar, 10 μm the quantities of Hsf1 and chaperones or their mRNAs. For example, active (phosphorylated) and inactive forms of Hsf1 increase in the cold hardy larvae of the goldenrod gall moth, Epiblema scudderiana as winter approaches and the insect enters diapause, this increase coinciding with augmentation of several molecular chaperones (Zhang et al 2018). In the larvae of another cold hardy goldenrod gall moth, Eurosta solidaginis, activated Hsf1 increases in coordination with several molecular chaperones as temperatures cool and diapause ensues, but unlike in E. scudderiana, Hsf1 declines in midwinter and remains low until spring (Zhang et al 2011).…”

Section: Discussionmentioning

confidence: 99%

The synthesis of diapause-specific molecular chaperones in embryos of Artemia franciscana is determined by the quantity and location of heat shock factor 1 (Hsf1)

Tan

MacRae

2019

Cell Stress and Chaperones

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The crustacean, Artemia franciscana, displays a complex life history in which embryos either arrest development and undertake diapause as cysts or they develop into swimming nauplii. Diapause entry is preceded during embryogenesis by the synthesis of specific molecular chaperones, namely the small heat shock proteins p26, ArHsp21, and ArHsp22, and the ferritin homolog, artemin. Maximal synthesis of diapause-specific molecular chaperones is dependent on the transcription factor, heat shock factor 1 (Hsf1), found in similar amounts in cysts and nauplii newly released from females. This investigation was performed to determine why, if cysts and nauplii contain comparable amounts of Hsf1, only cyst-destined embryos synthesize diapausespecific molecular chaperones. Quantification by qPCR and immunoprobing of Western blots, respectively, demonstrated that hsf1 mRNA and Hsf1 peaked by day 2 post-fertilization in embryos that were developing into cysts and then declined. hsf1 mRNA and Hsf1 were present in nauplii-destined embryos on day 2 post-fertilization, but in much smaller amounts than in cystdestined embryos, and they increased in quantity until release of nauplii from females. Immunofluorescent staining revealed that the amount of Hsf1 in nuclei was greatest on day 4 post-fertilization in cyst-destined embryos but could not be detected in nuclei of nauplius-destined embryos at this time. The differences in quantity and location of Hsf1 explain why embryos fated to become cysts and eventually enter diapause synthesize p26, ArHsp21, ArHsp22, and artemin, whereas nauplius-destined embryos do not produce these molecular chaperones.

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Elevated chaperone proteins are a feature of winter freeze avoidance by larvae of the goldenrod gall moth, Epiblema scudderiana

Cited by 11 publications

References 43 publications

Out in the Cold: Identification of Genomic Regions Associated With Cold Tolerance in the Biocontrol Fungus Clonostachys rosea Through Genome-Wide Association Mapping

Out in the Cold: Identification of Genomic Regions Associated With Cold Tolerance in the Biocontrol Fungus Clonostachys rosea Through Genome-Wide Association Mapping

Transcriptomic and proteomic analyses of the mechanisms of overwintering diapause in soybean pod borer (Leguminivora glycinivorella)

The synthesis of diapause-specific molecular chaperones in embryos of Artemia franciscana is determined by the quantity and location of heat shock factor 1 (Hsf1)

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