Elevated extracellular calcium increases expression of bone morphogenetic protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells

Tada, Hiroyuki; Nemoto, Eiji; Kanaya, Sousuke; Hamaji, Nozomu; Sato, Hisae; Shimauchi, Hidetoshi

doi:10.1016/j.bbrc.2010.03.135

Cited by 69 publications

(63 citation statements)

References 30 publications

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“…Other studies have shown that extracellular Ca 2+ increased the mineralization of osteoblasts and mesenchymal stem cells [38,39]. In addition, the extracellular free ionized Ca 2+ may promote an increase in intracellular Ca 2+ through the calcium channels, resulting in the activation of numerous targets [40]. In a current study, Gabusi et al [41] reported that a high concentration of Ca 2+ intracellular increased ALP activity as well as collagen-I, osteocalcin, and sialoprotein expression in human osteoblasts.…”

Section: Discussionsupporting

confidence: 46%

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

et al. 2013

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The aim of the present study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like MDPC-23 cells exposed to carbamide peroxide (CP 0. 01 %-2.21 μg/mL of H 2 O 2 ). The cells were seeded in sterile 24-well plates for 72 h. Eight groups were established according to the exposure or not to the bleaching agents and the laser energy doses tested (0, 4, 10, and 15 J/cm 2 ). After exposing the cells to 0.01 % CP for 1 h, this bleaching solution was replaced by fresh culture medium. The cells were then irradiated (three sections) with a near-infrared diode laser (InGaAsP-780±3 nm, 40 mW), with intervals of 24 h. The 0.01 % CP solution caused statistically significant reductions in cell metabolism and alkaline phosphate (ALP) activity when compared with those of the groups not exposed to the bleaching agent. The LLLT did not modulate cell metabolism; however, the dose of 4 J/cm 2 increased the ALP activity. It was concluded that 0.01 % CP reduces the MDPC-23 cell metabolism and ALP activity. The LLLT in the parameters tested did not influence the cell metabolism of the cultured cells; nevertheless, the laser dose of 4 J/cm 2 increases the ALP activity in groups both with and without exposure to the bleaching agent.

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Section: Discussionsupporting

confidence: 46%

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

et al. 2013

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“…We postulated that ERK might be involved in genipin-induced differentiation because ERK has been reported as an essential mediator of differentiation in hDPCs (33)(34)(35)(36). In this respect, the present study examined whether the ERK pathway is involved in genipin-induced odontogenic differentiation of hDPCs.…”

Section: Discussionmentioning

confidence: 95%

Genipin, a Cross-linking Agent, Promotes Odontogenic Differentiation of Human Dental Pulp Cells

Kwon

Lim

Kim

et al. 2015

Journal of Endodontics

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“…PO 4 2- is also a regulator of osteoblast proliferation and differentiation [35]. Periodontal ligament and pulp cells stimulated with extracellular Ca 2+ and for 24 h, PO 4 2- also increased bone morphogenetic protein-2 (BMP-2) mRNA expression [5,36]. …”

Section: Discussionmentioning

confidence: 99%

Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

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Aimsto design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease.MethodsPolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached to an energy dispersive analysis system. Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests.ResultsPrecipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic effect.ConclusionsThe ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity may offer new strategies for periodontal disease treatment.

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Elevated extracellular calcium increases expression of bone morphogenetic protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells

Cited by 69 publications

References 30 publications

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

Effect of low-level laser therapy on odontoblast-like cells exposed to bleaching agent

Genipin, a Cross-linking Agent, Promotes Odontogenic Differentiation of Human Dental Pulp Cells

Bioactive Polymeric Nanoparticles for Periodontal Therapy

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