Elevated lysine crotonylation and succinylation in the brains of BTBR mice

Wang, Min; Chang, Qiaoqiao; Yang, Hua; Liu, Yongfeng; Wang, Chunfang; Hu, Fengyun; Wei, Huijie; Li, Rongshan

doi:10.1016/j.ijdevneu.2019.06.011

Cited by 11 publications

(9 citation statements)

References 28 publications

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“…BTBR T Itpr3tf/J (BTBR) mice have developmental disorders in the central nervous system and show many abnormal neuroanatomical structures. Compared with B6 mice, the global Kcr level of the cerebral cortex of BTBR mice is increased [ 103 ], suggesting that the functional relationship between crotonylation and brain diseases is of great significance. About 70 kDa of proteins in mouse brain extract can be recognized by pan Kcr antibodies, indicating the crotonylated non-histone proteins in the brain [ 104 ].…”

Section: Introductionmentioning

confidence: 99%

Emerging roles of non-histone protein crotonylation in biomedicine

Hou

Zhou

et al. 2021

Cell Biosci

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Crotonylation of proteins is a newly found type of post-translational modifications (PTMs) which occurs leadingly on the lysine residue, namely, lysine crotonylation (Kcr). Kcr is conserved and is regulated by a series of enzymes and co-enzymes including lysine crotonyltransferase (writer), lysine decrotonylase (eraser), certain YEATS proteins (reader), and crotonyl-coenzyme A (donor). Histone Kcr has been substantially studied since 2011, but the Kcr of non-histone proteins is just an emerging field since its finding in 2017. Recent advances in the identification and quantification of non-histone protein Kcr by mass spectrometry have increased our understanding of Kcr. In this review, we summarized the main proteomic characteristics of non-histone protein Kcr and discussed its biological functions, including gene transcription, DNA damage response, enzymes regulation, metabolic pathways, cell cycle, and localization of heterochromatin in cells. We further proposed the performance of non-histone protein Kcr in diseases and the prospect of Kcr manipulators as potential therapeutic candidates in the diseases.

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Section: Introductionmentioning

confidence: 99%

Emerging roles of non-histone protein crotonylation in biomedicine

Hou

Zhou

et al. 2021

Cell Biosci

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“…Convertion of SCFA crotonate into crotonyl-CoA is mediated by Acyl-CoA synthetase short chain family member 2 (ACSS2) or other metabolic pathways, such as fatty acid β-oxidation pathway and the lysine degradation pathway (Sabari et al, 2015) (Lin et al, 2012). Currently, the data featured in the literatures show that KCr modi cations are involved in the aberrant neuroanatomical structures of BTBR mice (Wang et al, 2019a), depressive disorder of male mice (Liu et al, 2019) in the central nervous system. At the same time, we also observed two increased KCr modi cations bands adjacent to 40 and 35kDa at day 7 and 21 after HFD.…”

Section: Discussionmentioning

confidence: 99%

Short-Term High-Fat Diet Promotes Increased Lysine Crotonylation in Cerebral Cortex

Wang

Zhang

Wang

et al. 2021

Preprint

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Protein lysine crotonylation is a newly discovered protein post-translational modification (PTM), which has been associated with cellular metabolism, cell cycle, gene transcription, DNA damage response. However, its potential roles related to human central nervous system diseases remain largely unknown. In the present study, we observed a significant elevated lysine crotonylation in a screening of nine lysine acylations in cortex tissues of HFD-fed mice after short-term overfeeding. On the base of previous reports and molecular weight of proteins, we also speculate that actin, ERK2 or GAPDH and CDK1 might be modified by lysine crotonylation (KCr). Taken together, our findings highlight a potential role of protein lysine crotonylation in HFD-induced brain disorders and as possible therapeutic candidates in the future.

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“… 573 Crotonylation and succinylation levels are increased in the cerebral cortex of mice with developmental disorders of the central nervous system (CNS). 574 Neural excitation can modulate Kla levels in brain cells, suggesting that protein Kla in the brain may be associated with neuropsychiatric disorders. 575 CDYL mediates histone crotonylation, which regulates gene transcription and promotes the development of stress‐induced depression in rodents, providing a potential therapeutic target for major depression.…”

Section: Other Scfa Modificationsmentioning

confidence: 99%

Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications

Zhong

Xiao

Xie

et al. 2023

MedComm

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Protein posttranslational modifications (PTMs) refer to the breaking or generation of covalent bonds on the backbones or amino acid side chains of proteins and expand the diversity of proteins, which provides the basis for the emergence of organismal complexity. To date, more than 650 types of protein modifications, such as the most well‐known phosphorylation, ubiquitination, glycosylation, methylation, SUMOylation, short‐chain and long‐chain acylation modifications, redox modifications, and irreversible modifications, have been described, and the inventory is still increasing. By changing the protein conformation, localization, activity, stability, charges, and interactions with other biomolecules, PTMs ultimately alter the phenotypes and biological processes of cells. The homeostasis of protein modifications is important to human health. Abnormal PTMs may cause changes in protein properties and loss of protein functions, which are closely related to the occurrence and development of various diseases. In this review, we systematically introduce the characteristics, regulatory mechanisms, and functions of various PTMs in health and diseases. In addition, the therapeutic prospects in various diseases by targeting PTMs and associated regulatory enzymes are also summarized. This work will deepen the understanding of protein modifications in health and diseases and promote the discovery of diagnostic and prognostic markers and drug targets for diseases.

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Elevated lysine crotonylation and succinylation in the brains of BTBR mice

Cited by 11 publications

References 28 publications

Emerging roles of non-histone protein crotonylation in biomedicine

Emerging roles of non-histone protein crotonylation in biomedicine

Short-Term High-Fat Diet Promotes Increased Lysine Crotonylation in Cerebral Cortex

Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications

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