Elevated Spermidine And Spermine Levels In The Blood Of Psoriasis Patients

Proctor, Michael S.; Fletcher, Howard Vance; Shukla, Jayesh B.; Rennert, Owen M.

doi:10.1111/1523-1747.ep12607659

Cited by 66 publications

(20 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[11] Polyamine levels in blood, urine, and other biological fluid fluctuated in accordance with psoriasis activity. [12] SPD/SM ratio, considered as an indicator of proliferation activity, was found increased in psoriatic lesions, compared to non-lesional skin of patients affected by the disease. [13] Interestingly, patients with psoriasis receiving etretinate showed significant drop of all urinary polyamines, with the greatest prevalence of SM.…”

Section: Discussionmentioning

confidence: 99%

Polyamine metabolism changes in psoriasis

2013

View full text Add to dashboard Cite

Introduction:Polyamines – putrescine, spermidine and spermine are polycationic compounds ubiquitous for all living organisms. They are essential for the cell growth and differentiation, the control of cell cycle progress, apoptosis, and cancerogenesis. Accumulated scientific evidence suggests the central role of polyamines in the process of keratinocytic proliferation, differentiation, and regulation.Objective:To elucidate the polyamine metabolic changes that occur in benign keratinocytic proliferation. Fifty eight patients were enrolled in the study, 31 with plaque-form of psoriasis vulgaris, which had been referred to as a model of benign keratinocytic proliferation, and 27-healthy controls.Materials and Methods:An original, innovative chromatographic method was used to detect the levels of putrescine, spermidine, and spermine in all skin samples.Results:Were significantly proven (P < 0.05). No difference was found between the polyamines levels of non-lesional psoriatic skin and healthy controls. Psoriatic lesions showed a two-time higher concentration of all polyamines in lesional, compared to non-lesional skin. Spermine had the highest concentration and highest proliferation trend, which demonstrated the importance of propylamine synthesis in the pathogenesis of psoriasis. Spermine highest concentrations suggested the leading role of adenosine methionine decarboxylase (AMDC) in the pathogenesis of benign keratinocytic proliferations.Conclusions:Non-lesional skin in psoriatic patients did not show latent changes in polyamine metabolism. Psoriatic lesions demontrated two-time higher levels of the most essential biogenic polyamines compared to healthy controls. The highest level of spermine proved the crucial role of AMDC in the polyamine metabolism changes in psoriasis. Future therapeutic approaches should be focused on reduction of exogenic spermine intake, utilizing new spermine blockers, and synthesis of AMDC inhibitors.

show abstract

Section: Discussionmentioning

confidence: 99%

Polyamine metabolism changes in psoriasis

2013

View full text Add to dashboard Cite

show abstract

“…Callus extracts were minimally stimulated. Since spermidine and spermine levels have been reported to be elevated in psoriatic plaques (Proctor et al, 1975), it is possible that the higher ribonuclease levels observed in psoriatic extracts may reflect higher endogenous polyamine levels. If the psoriatic ribonucleases are already optimally stimulated, further addition of polyamines may raise the polyamine concentration to a value which depresses activity (Fig.…”

Section: Discussionmentioning

confidence: 99%

Epidermal Nucleases. II. The Multiplicity of Ribonucleases in Guinea-Pig Epidermis

Melbye

Freedberg

1977

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Ribonuclease activity has been extracted from adult guinea-pig epidermis by sequential homogenization in dilute sodium acetate and sulfuric acid. The extracts were subjected to ammonium sulfate fractionation and to affinity and ion exchange chromatography. Three ribonucleases (I, II, III) were separated from the sodium acetate extract and 6(A, B1, B2, B3, C, D) were isolated from the sulfuric acid extract. The degree of purification varies from 65-fold to 8,700-fold and the apparent molecular weights of the active forms of 8 of the 9 ribonucleases range from 10,000 to 36,500. No phosphodiesterase activity is present in any of the 9 fractions, but there is alkaline phosphatase activity in one (I) and deoxyribonuclease activity in a second (B3). Two of the ribonucleases have acid pH optima (a1, B3), while the others are most active between PHs 6.8 and 7.8. The activity of 4 of the fractions is sensitive to added EDTA (III, A, B2, B3,), but no stimulatory metal ions were found. Low concentrations of the polyamine spermidine enhanced the activity of 3-fractions (III, C, D). Yeast ribonucleic acid is degraded exonucleolytically by 2 fractions (I, A) and endonucleolytically by the remaining 7. In experiments with homopolyribonucleotide substrates, poly U was generally the preferred substrate. Substantial hydrolysis of poly A occurred with 2 fractions (A, B3) and slight hydrolysis of poly G with 2 other fractions (B2, C).

show abstract

“…The concentration of spermine in the whole blood of psoriatic patients is increased twoto threefold above normal, and there is also an elevation of spermidine (PROCTOR et al 1975;COOPER et al 1978). More detailed investigation (COOPER et al 1978) reveals increased amounts associated with the erythrocytes but not with the leucocytes.…”

Section: Polyamine Metabolismmentioning

confidence: 92%

“…Early findings using only a few subjects actually suggested a reduction of skin polyamines in psoriasis (STiiTTGEN 1968;PROCTOR et al 1975), but all other studies have shown a statistically significant increase in putrescine, spermidine and spermine over normal controls in involved psoriatic epidermis (RUSSELL et al 1978 b;BOHLEN et al 1978;GROSSHANS et al 1978;PROCTOR et al 1979;LAUHARANTA et al 1981). Early findings using only a few subjects actually suggested a reduction of skin polyamines in psoriasis (STiiTTGEN 1968;PROCTOR et al 1975), but all other studies have shown a statistically significant increase in putrescine, spermidine and spermine over normal controls in involved psoriatic epidermis (RUSSELL et al 1978 b;BOHLEN et al 1978;GROSSHANS et al 1978;PROCTOR et al 1979;LAUHARANTA et al 1981).…”

Section: Polyamine Metabolismmentioning

confidence: 99%