Elicitation of Taxus sp. cell cultures for production of taxol

Veeresham, Ciddi; Srinivasan, Venkatesh; Shuler, Michael L.

doi:10.1007/bf00189223

Cited by 76 publications

(38 citation statements)

References 11 publications

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“…A concentration of 200 μM MeJA is recommended for improved the expression of these genes. Moreover, the timing of the application of MeJA is critical [48,49]. Our experimental results showed that treating S. grosvenorii fruit with MeJA at 30 DAF was better than at 10 or 20 DAF since six of the seven groups at 30 DAF showed a positive response to the stimulus (Fig.…”

Section: Discussionmentioning

confidence: 73%

Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages

et al. 2016

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The cucurbitane-type triterpenoid glycosides, mogrosides, are the main active components of Siraitia grosvenorii fruit. Squalene and cucurbitadienol are among the intermediates of the biosynthetic pathway for the formation of cucurbitanetype triterpenoid backbones of mogrosides. It is recognized that the exogenous application of methyl jasmonate (MeJA) increases the accumulation of secondary metabolites in various plant species. Here, the effect of MeJA (50, 200, and 500 μM) on the accumulation of squalene and cucurbitadienol in the fruits of S. grosvenorii at 10, 20, and 30 days after flowering (DAF) was tested for the first time. Since mogroside II E is the main cucurbitane-type triterpenoid present at this time, its concentration was also determined. The results show that MeJA can indeed promote squalene and cucurbitadienol accumulation, the application of 500 μM MeJA at 30 DAF being optimal. The concentration of squalene and cucurbitadienol increased up to 0.43 and 4.71 μg/g dry weight (DW), respectively, both of which were 1.2-fold greater than that of the control. The content of mogroside II E increased by 15% over the untreated group. We subsequently analyzed the expression of key genes involved in the mogroside biosynthetic pathway, including the 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene (SgHMGR), squalene synthetase gene (SgSQS), cucurbitadienol synthase gene (SgCS), and cytochrome P450 (SgCYP450) with quantitative real-time PCR. The results showed that transcriptional levels of these genes were upregulated following the treatment described above. Additionally, their responses in the presence of MeJA was related to the concentration and timing of MeJA treatment.

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Section: Discussionmentioning

confidence: 73%

Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages

et al. 2016

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“…MeJA, SA, hydrogen peroxide and fungal extract have been reported to be capable of inducing the production of taxol (Stierle et al, 1993;Ciddi et al, 1995;Yukimune et al, 1996;Yu et al, 2001), but the mechanisms of these elicitors have not been completely understood. Especially, little has been reported on the relation between the above elicitors and the activity of cytochrome P450 monooxygenases or acetyltransferases.…”

Section: Introductionmentioning

confidence: 99%

Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyl transferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells

Zhang¹,

Gong²,

Guo³

2010

Cell Biology Int.

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The effects of four elicitors, including 100 mmol/l MeJA (methyl jasmonate), 40 ml/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.

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“…The most promising longterm solution to taxol production seems to be from cell suspension cultures using modem biotechnology approaches (ShortIe and Minocha, 1999). Ciddi et al (1995) showed that the addition of several fungal cell extracts and culture filtrates resulted in an increase in the production of taxol in cell cultures of Taxus. Yukimune et al, (1996) were able to achieve increased production of taxol and its precursor baccatin III in taxus cell cultures by adding methyl jasmonate.…”

Section: Secondary Metabolite Productionmentioning

confidence: 98%