Elicitation of Therapeutic Potential and Oxidative Stress Assessment of Fenugreek Sprouts Under Uv Irradiation

Abstract

Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidative stress status of fenugreek seedlings during the post-germination phase. Methods:The seeds of Trigonella foenum-graecum L. were subjected to UV radiation of different wavelengths UV 254 nm, UV 365 nm and the combination of 254 nm and 365 nm UV during the course of germination up to 96 h. Free radicals scavenging activity, in vitro antidiabetic activity as well as total phenol (TPC) and flavonoid (TFC) contents were evaluated up to four days of germination stages. Analysis of total activities of superoxide dismutase (SOD), peroxidase, catalase and NADPH oxidase (NOX) was conducted using non-denaturing polyacrylamide gel electrophoresis along with histochemical detection of H2O2 and superoxide radical localisation, lipid peroxidation and plasma membrane integrity was also performed. Results:Overall it was observed that the sprouts exposed to UV 365 nm exhibited a significant enhancement in free radical scavenging as well as antidiabetic activity at the 48h stage of germination. Interestingly highest phenol and flavonol content were also stimulated at the same stage by UV 365 nm. The effect of UV irradiation was evident on the localisation of H2O2 and superoxide radical. The on gel assay revealed that the total activities of the antioxidant enzymes were extensively elevated by UV irradiation. Conclusion:The results suggest that the UV irradiation technology can be implemented for the enhancement of nutraceutical properties of sprouts also UV exposure helps in the induction of the antioxidant enzyme system which may be beneficial for oxidative stress management in plants during germination phases.

Nephroprotective Activity of Plumeria Rubra Against Cisplatin Induced Nephrotoxicity in Experimental Rats

“…Plants have evolved various protective mechanisms for minimising deleterious effects of free radicals. The enzymatic defence comprises of the efficient antioxidant enzymes such as catalase, peroxidase, superoxide dismutase [31].…”

Host–Guest Encapsulation of RIBO with TSC4X: Synthesis, Characterization, and Its Application by Physicochemical and Computational Investigations

“…Metal Chelating Activity. Spectrophotometric assay for ferrous ions (Fe 2+ ) chelation activity of the tested sample was carried out according to Gupta et al 37 Test sample (400 μL) was mixed with methanol (1600 μL) and 2 mM of FeCl 2 (40 μL) and mixed well. Simultaneously, ferrozine (5 mM, 800 μL) was added to the existing mixture and after 10 min, the absorbance of the resultant solution was read at 562 nm.…”

Exploring the ameliorative role of Equisetum diffusum D. Don whole-plant methanolic-extract in acute inflammation and molecular docking analysis of GC-MS-identified phytocompounds with few prominent inflammatory markers/cytokines for inspecting the potent drug targets.

“…The chelating activity of the sample extract for ferrous ions (Fe +2 ) was spectrophotometrically measured as per standard protocol (Gupta et al, 2017). To 400 µl of EDME, 1600 µl of methanol, and 40 µl of FeCl 2…”