Elicitation of Trigonelline and 4-Hydroxyisoleucine with Hypoglycemic Activity in Cell Suspension Cultures of Trigonella foenum graecum L

Mawla, Ahmed M.A. Abd-El

doi:10.2174/2210289201102010080

Cited by 17 publications

(3 citation statements)

References 26 publications

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“…The important constituents of fenugreek include 4-hydroxyisoleucine (4-HI), trigonelline, galactomannan flavonoids, carotenoids, coumarins, proteins, saponins and lipids (Basch, Ulbricht, Kuo, Szapary, & Smith, 2003). Trigonelline controls the diabetes through operation of the mechanism of insulin secretion, modulation of β cell regeneration and stimulation of activity of glucose metabolism-related enzymes (Abd El-Mawla & Osman, 2011;Zhou, Chan, & Zhou, 2012). The seeds of fenugreek contain about 50% of fibre that is known to slow the rate of post-parandial glucose absorption, which may be responsible for the hypoglycaemic activity.…”

Section: Introductionmentioning

confidence: 99%

Cumulative effect of gibberellic acid and phosphorus on crop productivity, biochemical activities and trigonelline production in Trigonella foenum-graecum L.

Dar

Uddin

Khan

et al. 2015

Cogent Food & Agriculture

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Fenugreek (Trigonella foenum-graecum L.) is an antidiabetic plant. Its bioactive compound, trigonelline, is known to counter diabetes through insulin secretion, modulation of β cell regeneration and quick activity of glucose metabolism related enzymes. A pot experiment was conducted in the natural conditions of net house of the Department of Botany, Aligarh Muslim University, Aligarh (UP), India, to evaluate the effect of four concentrations of GA 3 (0, 10 −7 M, 10 −6 M and 10 −5 M), alone and in combination with phosphorus (40 kg P ha −1 ), on growth, biochemical and yield attributes of fenugreek. Compared to control, the combination of GA 3 and phosphorus (P40 + 10 −6 M GA3) significantly increased the activities of nitrate reductase (30.8%) and carbonic anhydrase (30.7%) enzymes; it also enhanced the seed yield (140.6%) and the content of total chlorophyll (28.5%) and carotenoids (26%). There was also significant increase (19.51%) in the content of seed trigonelline.

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Section: Introductionmentioning

confidence: 99%

Cumulative effect of gibberellic acid and phosphorus on crop productivity, biochemical activities and trigonelline production in Trigonella foenum-graecum L.

Dar

Uddin

Khan

et al. 2015

Cogent Food & Agriculture

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“…Fowden et al (1973) isolated and identified the amino acid, 4hydroxyisoleucine, in fenugreek seed for the first time. AbdelMawla and Osman (2011) used cell suspension cultures to raise the con centration of 4hydroxyisoleucine content in fenugreek seed, which increased serum insulin levels and showed improvement in lowering the fasting blood glucose level in streptozotocin (STZ)treated diabetic rats. AbdelMawla and Osman (2011) used cell suspension cultures to raise the con centration of 4hydroxyisoleucine content in fenugreek seed, which increased serum insulin levels and showed improvement in lowering the fasting blood glucose level in streptozotocin (STZ)treated diabetic rats.…”

Section: The Alkaloid-trigonellinementioning

confidence: 99%

Fenugreek

Garg¹

2016

Nutraceuticals

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“…The induction effect of both MJ and Q can be suppressed by pre-incubation of hairy root cultures with salicylic acid. Both Trigonelline and 4-Hydroxyisoleucine ( Figure 11) with Hypoglycemic Activity were elicited in Cell Suspension Cultures of Trigonella foenum graecum L. [128]. Cell suspension cultures of Trigonella foenum graecum L. (fenugreek) were initiated from the cotyledon portions of sterile germinated seeds and maintained on MS medium supplemented with 2, 4-dichlorophenoxy acetic acid (2, 4-D) (1mg L -1 ), kinetin (0.1 mg L -1 ) and sucrose (5%).…”

Section: Cardiac Glycosides From Cryptostegiamentioning

confidence: 99%

Bioactive secondary metabolites produced in the author's laboratory by tissue culture techniques

El-Mawla¹

2014

Spatula DD

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Plant tissue culture techniques are used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture relies on the fact that many plant cells have the ability to regenerate a whole plant. Single cells, plant cells without cell walls (protoplasts), pieces of leaves, stems or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Cultured plant cells can be offered alternative sources over whole plant cultivation for the production of secondary metabolites. The explants, such as plant leaves, stems, roots, meristems etc. were used for cultivation and establishment of plant cell and tissue cultures. Unfortunately, most often plant cell cultures technologies not lead to an enhancement in secondary metabolites production. Therefore, Strain improvement, methods for the selection of high-producing cell lines, and medium optimizations must be considered to improve the production of secondary metabolites. Several types of cell culture methods are being used to produce bioactive compounds. Cell suspension cultures are preferred for production large amounts of cells for quantitative or qualitative analytical studies. This review refers to certain bioactive secondary metabolites produced in the author's laboratory by different tissue cultures techniques. ÖZETBitki dokusu kültür teknikleri bitki hücrelerini, dokularını veya organlarını bilinen bileşenlerde besleyici kültür ortamlarında steril şartlarda korumak ve beslemek için kullanılırlar. Bitki doku kültürleri, birçok bitki hücresinin tüm bir bitkiyi yeniden oluşturma yeteneğine sahip olması gerçeğine dayanır. Tek başlarına hücreler, hücre duvarı (protoplast) olmayan bitki hücreleri, yaprak parçaları, sap veya kökler gerekli besleyiciler ve bitki hormonları verilen kültür ortamlarında yeni bir bitki üretmek için sıklıkla kullanılabilmektedir. Kültüre edilmiş bitki hücreleri, sekonder metabolitlerin üretimi için tüm bitki yetiştirilmesi süresince alternatif bir kaynak olarak ortaya çıkabilir. Bitki yaprakları, kökler, saplar, filizler vb. gibi eksplantlar, bitki hücre ve doku kültürlerinin yetiştirilmesi ve kurulmasında kullanılmaktadır. Maalesef, çoğu zaman bitki hücre kültürü teknolojileri sekonder metabolit üretiminde bir artışa neden olmamaktadır. Bu yüzden suş geliştirme, yüksek üretilebilir hücre hatlarının seçimi için metotlar ve vasat optimizasyonu gibi yöntemler sekonder metabolit üretiminin geliştirilmesi için dikkate alınmalıdır. Biyoaktif bileşiklerin üretimi için birkaç tipte hücre kültürü kullanılmaktatır. Kantitatif ve kalitatif analitik çalışmalarda büyük miktarda hücre üretimi için hücre süspansiyon kültürleri tercih edilmektedir. Bu derleme, farklı doku kültür teknikleri sayesinde laboratuarlarda üretilen belirli biyoaktif sekonder metabolitlere değinmektedir. Anahtar kelimeler: Hücre süspansiyon kültürleri, filiz kültürleri, saçlı kök kültürleri, biyoaktif bileşikler.

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Elicitation of Trigonelline and 4-Hydroxyisoleucine with Hypoglycemic Activity in Cell Suspension Cultures of Trigonella foenum graecum L

Cited by 17 publications

References 26 publications

Cumulative effect of gibberellic acid and phosphorus on crop productivity, biochemical activities and trigonelline production in Trigonella foenum-graecum L.

Cumulative effect of gibberellic acid and phosphorus on crop productivity, biochemical activities and trigonelline production in Trigonella foenum-graecum L.

Fenugreek

Bioactive secondary metabolites produced in the author's laboratory by tissue culture techniques

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