Elucidating the fate of perfluorooctanoate sulfonate using a rainbow trout (Oncorhynchus mykiss) physiologically-based toxicokinetic model

Vidal, Alice; Babut, Marc; Garric, Jeanne; Beaudouin, Rémy

doi:10.1016/j.scitotenv.2019.07.105

Cited by 22 publications

(7 citation statements)

References 56 publications

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“…Therefore, it can be inferred that the high accumulation of PFAS in blood and the liver may be caused by reabsorption during the enterohepatic circulation involving the key target proteins NTCP and ASBT. However, these proteins have not been considered in most previous studies using the physiologically based pharmacokinetic (PBPK) model for PFAS. − Only Chen and Ng selected NTCP as an important reabsorption target to construct the parameters of the PBPK model for PFOA in male rats …”

Section: Resultsmentioning

confidence: 99%

Effect of Enterohepatic Circulation on the Accumulation of Per- and Polyfluoroalkyl Substances: Evidence from Experimental and Computational Studies

Cao

Zhou

et al. 2022

Environ. Sci. Technol.

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The pharmacokinetic characteristics of per- and polyfluoroalkyl substances (PFAS) affect their distribution and bioaccumulation in biological systems. The enterohepatic circulation leads to reabsorption of certain chemicals from bile back into blood and the liver and thus influences their elimination, yet its influence on PFAS bioaccumulation remains unclear. We explored the role of enterohepatic circulation in PFAS bioaccumulation by examining tissue distribution of various PFAS in wild fish and a rat model. Computational models were used to determine the reabsorbed fractions of PFAS by calculating binding affinities of PFAS for key transporter proteins of enterohepatic circulation. The results indicated that higher concentrations were observed in blood, the liver, and bile compared to other tissues for some PFAS in fish. Furthermore, exposure to a PFAS mixture on the rat model showed that the reabsorption phenomenon appeared during 8–12 h for most long-chain PFAS. Molecular docking calculations suggest that PFAS can bind to key transporter proteins via electrostatic and hydrophobic interactions. Further regression analysis adds support to the hypothesis that binding affinity of the apical sodium-dependent bile acid transporter is the most important variable to predict the human half-lives of PFAS. This study demonstrated the critical role of enterohepatic circulation in reabsorption, distribution, and accumulation of PFAS.

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Section: Resultsmentioning

confidence: 99%

Effect of Enterohepatic Circulation on the Accumulation of Per- and Polyfluoroalkyl Substances: Evidence from Experimental and Computational Studies

Cao

Zhou

et al. 2022

Environ. Sci. Technol.

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“…This might be due to the following reasons: First, in addition to PFOSA, there are other N -substituted PFOSA derivatives and other unknown PreFOSs in the environment, which may also make contributions to PFOS in organisms . Second, in addition, through water exposure via fish gill, ingestion of prey and suspended matters could be an important exposure pathway of PFOS and PreFOSs in aquatic organisms, while the kinetic parameters of PFOS and PreFOSs via these exposure pathways could be distinctly different from gill exposure. ,, …”

Section: Resultsmentioning

confidence: 61%

“…PFOS usually exhibited higher concentration in Carp blood than in the muscle, and PFOS in carp blood would be 563 ng/g, according to the partition ratio of PFOS in blood and muscle . The great discrepancy in the theoretical and actual blood concentrations of PFOS in carp magnified a great contribution of dietary exposure for PFOS. , This was supported by studies on rainbow trout, which demonstrated that the uptake rate of PFOS via diet was much larger than that via gill. , In future, related kinetic constants of the isomers of PFOS and PreFOSs via dietary uptake should be considered to simulate the realistic exposure conditions.…”

Section: Resultsmentioning

confidence: 93%

Quantitative Estimation of Relative Contributions of Direct and Indirect Exposures to Perfluorooctane Sulfonate in Organisms Using the Isomer Profiling Technique

et al. 2022

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It remains a big challenge to estimate the relative importance of direct vs indirect (via precursors) exposures of animals and humans to perfluorooctane sulfate (PFOS). In this study, fish as a biological model were exposed to PFOS and its typical precursor, perfluorooctane sulfonamide (PFOSA), singly and combined with different ratios in water for 76 days, followed by a 21-day depuration. Isomer-specific kinetic parameters including uptake, elimination, and biotransformation rates as well as bioconcentration factor (BCF) and biotransformation accumulation factor (BTAF) of PFOS were obtained based on single exposures. Single exposure to PFOS or PFOSA led to an opposite change in the % n-PFOS (increase vs decrease) in the fish blood. Based on the obtained kinetic parameters of each isomer, a quantitative model describing the relationship between % n-PFOS in blood and the relative concentration percentage of PFOSA in water was established. As verified by the coexposure tests, the model exhibited a promising performance in quantitative prediction of relative contribution of precursor exposure in water to the body burden of PFOS in fish. Field application of the model suggested that in addition to gill uptake, dietary exposure should be considered to generate more accurate results.

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“…In the case of the aquatic environment, PFAS are taken up by sh via passive diffusion through the gills, and they associate with blood proteins in the circulatory system and extracellular matrices before being eliminated via three pathways: passive diffusion via the gill, urinary and biliary excretions (Ng and Hungerbühler, 2013;Qiang et al, 2016;Zhong et al, 2019). In rainbow trout, PFOA and PFOS have been found to preferentially accumulate in the liver following both dietary and ow-through exposures (Goeritz et al, 2013;Martin et al, 2003;Vidal et al, 2019). Their interaction with key molecular pathways and potential subsequent effects in sh remain largely unclear.…”

Section: Introductionmentioning

confidence: 99%

Effects of perfluoroalkyl substances (PFAS) and benzo[a]pyrene (BaP) co-exposure on phase I biotransformation in rainbow trout (Oncorhynchus mykiss)

Solan

Franco

Lavado

2022

Preprint

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The presence of perfluoroalkyl substances (PFAS) in the environment, especially in aquatic ecosystems, continues to be a significant concern for human and environmental health. Previous studies have suggested that several PFAS do not undergo biotransformation due to their chemical stability, yet perfluorooctanesulfonic acid (PFOS)- and perfluorooctanoic acid (PFOA)-exposed organisms have presented altered activity of important biotransformation pathways. Given the fundamental role of biotransformation in biological organisms and the significant distribution of PFAS in aquatic environments, the present study investigated the influence of PFOA and PFOS on phase I biotransformation enzymes in vitro using the rainbow trout liver RTL-W1 cell line and in vivo using juvenile rainbow trout. Cells and fish were exposed and co-exposed to environmentally relevant concentrations of PFOA, PFOS, and benzo[a]pyrene (BaP), for 72 h and 10 days, respectively, prior to measurements of cytotoxicity and biotransformation ability through measurements of CYP1A1-, CYP1A2- and CYP3A4-like activities. Our results indicate that exposure to PFAS-BaP binary mixtures altered CYP1A-like activity in vivo; however, those alterations were not observed in vitro. Similarly, while BaP did not significantly induce CYP3A4, exposure to the PFAS led to significantly lower enzymatic activity relative to basal levels. These observations may have implications for organisms simultaneously exposed to PFAS and other environmental pollutants for which biotransformation is necessary, especially in detoxification mechanisms. Furthermore, the interference with biotransformation pathways could potentially increase adverse outcomes, compromising the stability of fish populations inhabiting PFOA- and PFOS-polluted environments.

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Elucidating the fate of perfluorooctanoate sulfonate using a rainbow trout (Oncorhynchus mykiss) physiologically-based toxicokinetic model

Cited by 22 publications

References 56 publications

Effect of Enterohepatic Circulation on the Accumulation of Per- and Polyfluoroalkyl Substances: Evidence from Experimental and Computational Studies

Effect of Enterohepatic Circulation on the Accumulation of Per- and Polyfluoroalkyl Substances: Evidence from Experimental and Computational Studies

Quantitative Estimation of Relative Contributions of Direct and Indirect Exposures to Perfluorooctane Sulfonate in Organisms Using the Isomer Profiling Technique

Effects of perfluoroalkyl substances (PFAS) and benzo[a]pyrene (BaP) co-exposure on phase I biotransformation in rainbow trout (Oncorhynchus mykiss)

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