Emamectin benzoate (Proclaim®) mediates biochemical changes and histopathological damage in the kidney of male Wistar rats (Rattus norvegicus)

Khaldoun-Oularbi, Hassina; Allorge, Delphine; Richeval, Camille; Lhermitte, Michel; Djenas, Nadia

doi:10.1016/j.toxac.2014.11.002

Cited by 15 publications

(15 citation statements)

References 32 publications

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“…The above findings were confirmed by the observed histopathological changes. In accordance with our histopathological results, previous studies reported pathological changes in liver and kidney tissues of FIP- (Mossa et al, 2015;Abdel-Daim and Abdeen, 2018) and EMB- (Mahmoud and Mahmoud, 2010, Khaldoun-Oularbi et al, 2015 intoxicated rats.…”

Section: Discussionsupporting

confidence: 92%

Bee Pollen Alleviates Fipronil and Emamectin Benzoate Induced- Hepatorenal Toxicity in Rats

2019

Assiut Veterinary Medical Journal

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Section: Discussionsupporting

confidence: 92%

Bee Pollen Alleviates Fipronil and Emamectin Benzoate Induced- Hepatorenal Toxicity in Rats

2019

Assiut Veterinary Medical Journal

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“…9 We have recently demonstrated the toxic effect of EMB and abamectin in rats. 10,11 Our results demonstrated a toxic dose-dependent effect of EMB and abamectin with the accumulation of residues B1a and B1b in plasma and organs. Interestingly, after 14 days of non-treatment period, those residues were eliminated from the plasma, but persisted in kidney and liver tissues.…”

Section: Introductionmentioning

confidence: 52%

“…The tested concentration was chosen based on our preliminary experiments, using this experimental model. 11 The selection of vitamin C dose was done according to the study of Fetoui et al 16…”

Section: Methodsmentioning

confidence: 99%

“…Emamectin (B1a and B1b isomers) concentration was determined in plasma (at 14, 21, 28 and 42 days) and liver tissues (at 28 and 42 days), using a UHPLC–MS/MS method, according to a previously described procedure. 11 In brief, plasma samples were homogenized in acetonitrile. After centrifugation at 10,800 r/min for 8 min, the supernatant was evaporated under nitrogen.…”

Section: Methodsmentioning

confidence: 99%

“…Interestingly, after 14 days of non-treatment period, those residues were eliminated from the plasma, but persisted in kidney and liver tissues. 10,11 It is well known that AVMs are substrates for P-glycoprotein (P-gp)-mediated drug efflux. 12,13 Lespine et al showed that exposure of cells to P-gp substrates leads to an increase in reactive oxygen species (ROS) due to a higher adenosine triphosphate (ATP) use and the need for higher rates of ATP synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Ameliorative effect of vitamin C against hepatotoxicity induced by emamectin benzoate in rats

et al. 2016

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In the present study, we aimed to assess the potential protective effect of ascorbic acid (AA) against emamectin benzoate (EMB)-induced hepatotoxicity. For this purpose, biochemical, histopathological and analytical investigations were performed. Male Wistar rats were distributed into three groups, that is, a control group, an EMB group given 10 mg EMB/kg body weight (BW) by gavage and an EMB + AA group given 10 mg EMB/kg BW and vitamin C intraperitoneally (200 mg/kg). The duration of the treatment was 28 days and the duration of the study was 42 days. There was a statistically significant increase of all hepatic biomarkers, that is, aspartate aminotransferase, alanine aminotransferase and gamma-glutamyltransferase activities, and glycemia, in EMB-treated group when compared with the control group. Light microscopic observations revealed variable signs of hepatotoxicity in the EMB group, which were represented by alteration of normal hepatic architecture, inflammatory cell infiltration, hepatocellular steatosis and foci of necrosis at 28 and 42 days post-treatment. However, co-treatment with vitamin C reduced EMB-related liver toxicity and diminished the abnormal biochemical and architectural damage. Emamectin B1a and B1b residues were detectable in all plasma samples of treated rats at 14, 21 and 28 days of treatment. The drug liver tissue concentration was significantly lower in EMB + AA group compared with EMB group at 28 and 42 days. In conclusion, the findings of the present study clearly indicate a significant protective action of vitamin C against EMB hepatotoxicity.

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Emamectin benzoate as a potential molluscicide against white garden snail, Theba pisana in association with biochemical defects

Gad

2022

Pest Management Science

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BACKGROUND The white garden snail, Theba pisana, is distributed worldwide and is a serious molluscan pest of different crops. Emamectin benzoate (EMB) ‘an avermectin derivative’ is a novel biorational agent and highly effective pesticide. This study focused on the lethal and in vivo sublethal toxic effect of EMB on the energy reserves (glycogen, lipids and proteins), total energy reserves and activities of glutathione S‐transferase (GST), γ‐glutamyl transferase (γ‐GT), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the hepatopancreas of T. pisana for up to 7 days of exposure. RESULTS The median lethal dose (LD50) at 48 h of EMB treatment was 5.34 μg g−1 body weight (b.w.). Sublethal doses of 1.07 and 3.20 μg g−1 b.w. (i.e., 20% and 60% of the LD50) led to significant dose‐ and time‐dependent decreases in glycogen and lipids; these doses increased the total protein level. Overall, the tested sublethal doses significantly decreased the total energy reserves. Moreover, GST and γ‐GT activities were elevated, whereas the activities of AST and ALT were inhibited in the exposed snails. A decrease in LDH activity after 1 and 3 days of exposure and an increase after 7 days of exposure were seen in snails treated with EMB. CONCLUSION EMB exerted lethal toxicity on T. pisana and consequently caused changes in energy reserve levels and enzyme activities in the animal. © 2022 Society of Chemical Industry.

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Emamectin benzoate (Proclaim®) mediates biochemical changes and histopathological damage in the kidney of male Wistar rats (Rattus norvegicus)

Cited by 15 publications

References 32 publications

Bee Pollen Alleviates Fipronil and Emamectin Benzoate Induced- Hepatorenal Toxicity in Rats

Bee Pollen Alleviates Fipronil and Emamectin Benzoate Induced- Hepatorenal Toxicity in Rats

Ameliorative effect of vitamin C against hepatotoxicity induced by emamectin benzoate in rats

Emamectin benzoate as a potential molluscicide against white garden snail, Theba pisana in association with biochemical defects

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