Emblica officinalis Garten fruits extract ameliorates reproductive injury and oxidative testicular toxicity induced by chlorpyrifos in male rats

Abstract

Organophosphate pesticides have destroying properties on male reproduction and chlorpyrifos adversely affects the male reproductive system. Emblica offcinalis Garten plays a vital role to challenge many diseases in human body. We investigated the induction of oxidative stress in the male reproductive system of adult rats (Wistar Strain) exposed to widely used organophosphate pesticide, Chlorpyrifos, and tried to establish the ameliorative properties of Emblica officinalis Garten with respect to reproductive reconstruction in them. Rats were divided into 2 groups, control group and experimental group, and the experimental group was divided into 3 groups (G1-G3). All the groups had 5 rats each. Control group received water, experimental group, G1, received 20 mg/kg bw/day Emblica officinalis Garten, G2 received 12 mg/kg bw/day chlorpyrifos and G3 received 12 mg chlorpyrifos with 20 mg Emblica officinalis Garten /kg bw/day. Treatment was done orally from 30 days. Thereafter body weight, male reproductive organs weight, sperm count, sperm morphology, ACP, ALP, total protein, uric acid and testis and serum testosterone level were determined using standard methods. The changes recorded are indicative of infertility in male rats because of chlorpyrifos exposure. When the subjects were treated with Emblica officinalis Garten in conjunction with chlorpyrifos, these parameters exhibited recovery and when treated with Emblica officinalis Garten alone, these parameters were more or less near to the control group. This highlights the debilitating effect of chlorpyrifos and scavenging property of Emblica officinalis Garten.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-541) contains supplementary material, which is available to authorized users.

Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats

“…In agreement with previous studies, our histopathological examination of the testicular tissue revealed significant decline in the number of spermatogenic cells, Sertoli cells and inter‐tubular blood cells in CPF‐exposed groups (Dutta & Sahu, ; Elsharkawy et al, ; Mandal & Das, ; Mosbah et al, ; Sai et al, ). Among these cells, the mean spermatocyte and spermatid counts were significantly increased by some, and not all, treatment regimens in exercise‐trained groups when compared with the similarly treated sedentary animals (Figure e,f).…”

“…Some previous research works have shown that CPF administration leads to drastic histo‐morphologic changes in the testes of animal models, such as testicular atrophy, shrinkage of the seminiferous tubules, tubular diameter reduction and degenerative changes in the germinal epithelium (Dutta & Sahu, ; Elsharkawy, Yahia, & El‐Nisr, ; Farag et al, ; Mosbah, Yousef, Maranghi, & Mantovani, ). However, other studies have failed to detect some or all of these damages (Joshi et al, ; Kheradmandi, Jorsaraei, Feizi, Moghadamnia, & Neamati, ; Sai et al, ; Wen, Wei, & Pan, ).…”

Commercial Formulation of Chlorpyrifos Alters Neurological Behaviors and Fertility

“…Exposure to Judo 40 led to impaired sperm motility and nuclear integrity [11]. Hence, the fertilization capabilities of sperm in the present study were significantly reduced because of spermatozoa movement dysfunction and a decrease in sperm DNA integrity, and this is evident from the reduction in pregnancy indices [71].…”

“…Progressive motility of spermatozoa and nuclear integrity is extremely important for successful fertilization [67]. Increased head abnormalities [68,69] and impaired sperm motility [70] leads to a reduction in fertilizing ability [71,72]. Exposure to Judo 40 led to impaired sperm motility and nuclear integrity [11].…”

Augmenting fish health usingEmblica officinalisagainst triarylmethane dye induced blood toxicity inCyprinus carpio

“…Ameliorative efficacy of E. officinalis against chlorpyrifos and arsenic has also been demonstrated in animal models (Dutta & Sahu, ; Singh et al, ). Thus, it can be concluded from the present findings that MG adversely affects fish even at sublethal concentration and the negative impact of the dye can be reversed by diet supplementation of E. officinalis in C. carpio, thereby proving beneficial in aquaculture.…”