Emergence of distinct genetic variants in the population of primary Bartonella henselae isolates

Arvand, Mardjan; Schubert, H.; Viezens, Juliane

doi:10.1016/j.micinf.2005.12.015

Cited by 17 publications

(16 citation statements)

References 39 publications

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“…These two strains (CAL-1 and Fizz) are 98% homologous within the ITS region (971/983 bp) and 100% homologous within pap31 gene. This suggests that B. henselae strains CAL-1 and Fizz may belong to the same genetic variant, as suggested previously [2]. The rpoB gene sequences from Dog #1 were 99.8% homologous (655/656 bp) to B. henselae Houston-1.…”

Section: Brazil-1;supporting

confidence: 81%

“…In Brazil, B. quintana was detected by PCR in an infant [3] and two fatal cases of endocarditis were associated with high B. henselae antibody titers [41]. In contrast to humans, B. henselae seroreactivity was reported in 46% of 102 cats 2 suggesting that exposure to this organism in Brazil may be 1 Henn J.B., Koehler J.E., Gabriel M., Kasten R.W., Brown R.N., Papageorgiou S., Chomel B.B., Zoonotic Bartonella spp. in domestic dogs and gray foxes from California, in: Proc.…”

Section: Introductionmentioning

confidence: 99%

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Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection withBartonella henselaeandBartonella vinsoniisubsp.berkhoffii

et al. 2007

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-The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the São Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer (ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples (n = 8) were inoculated into a liquid pre-enrichment growth medium (BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog (ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii (pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs.dogs / Bartonella infections / heart disease / culture / Brazil

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Section: Brazil-1;supporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection withBartonella henselaeandBartonella vinsoniisubsp.berkhoffii

et al. 2007

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“…MLST was performed as previously described (Arvand et al, 2006;Iredell et al, 2003). In brief, the partial sequences of eight genetic loci, including 16S rRNA-DNA, batR, ftsZ, gltA, groEL, nlpD, ribC and rpoB, were analysed.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

“…We have recently demonstrated the coexistence of different genetic variants within the community of three primary B. henselae isolates from Germany by PFGE analysis of multiple single-colony-derived cultures (Arvand et al, 2006). No variants were detectable among the cultures obtained from isolates with a long passage history, suggesting that the inhomogeneous structure was restricted to primary isolates.…”

Section: Introductionmentioning

confidence: 99%

Bartonella henselae exists as a mosaic of different genetic variants in the infected host

et al. 2007

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Bartonella henselae is a fastidious bacterium associated with infections in humans and cats. The mechanisms involved in the long-term survival of bartonellae despite vigorous host immune responses are poorly understood. Generation of genetic variants is a possible strategy to circumvent the host specific immune responses. The authors have recently demonstrated the coexistence of different genetic variants within the progeny of three primary B. henselae isolates from Berlin by PFGE analysis. Aims of the present study were to determine whether coexistence of different variants is a common feature of B. henselae isolates worldwide and whether the genetic variants originally emerged in vivo. Thirty-four primary isolates from different geographical regions were analysed by subjecting multiple single-colony-derived cultures to PFGE analysis. Up to three genetic variants were detected within 20 (58.8 %) isolates, indicating that most primary isolates display a mosaic-like structure. The close relatedness of the genetic variants within an isolate was confirmed by multi-locus sequence typing. In contrast to the primary isolates, no genetic variants were detected within the progeny of 20 experimental clones generated in vitro from 20 primary isolates, suggesting that the variants were not induced in vitro during the procedure of PFGE analysis. Hence, the genetic variants within a primary isolate most likely originally emerged in vivo. Consideration of the mosaic structure of primary isolates is essential when interpreting typing studies on B. henselae.

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“…In a previous study, Kabeya et al (9) reported variations of up to 5 bands in PFGE patterns of B. henselae isolates obtained from naturally infected cats during different bacteremic peaks. However, we have recently demonstrated that genetic variants displaying 1-to 4-band differences frequently occur within primary B. henselae isolates and do not necessarily indicate infection by a different strain (11,12). Careful interpretation of PFGE typing results, use of additional restriction endonucleases, or use of other typing techniques is necessary to ensure correct classifi cation of different patterns to the variant or strain level.…”

mentioning

confidence: 99%

ProlongedBartonella henselaeBacteremia Caused by Reinfection in Cats

Arvand

Viezens

Berghoff

2008

Emerg. Infect. Dis.

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We analyzed the genetic relatedness of blood culture isolates of Bartonella henselae from 2 cats of patients with cat-scratch disease at admission and after 12 months. Isolates from each cat at different times were clonally unrelated, which suggested reinfection by a second strain.B artonella henselae is a zoonotic pathogen associated with a broad spectrum of disease manifestations in humans. Cat-scratch disease (CSD) is commonly encountered in immunocompetent patients; in immunocompromised patients, bacillary angiomatosis, peliosis hepatis, and recurrent bacteremia are usually seen. Domestic cats represent the main host and reservoir for B. henselae (1). Recurrent, intraerythrocytic bacteremia develops in infected cats without overt clinical symptoms (2). Experimental infection of specifi c pathogen-free cats with B. henselae induces recurrent episodes of bacteremia, which, in most cases, resolve spontaneously within 22-33 weeks postinfection (3-5). Prolonged bacteremia >7 months has been documented sporadically, e.g., in 1 of 12 experimentally infected cats inoculated with the highest infectious dose (this cat was bacteremic 32 weeks postinfection) (3), or in an unspecifi ed number of cases in 21 experimentally infected cats that were bacteremic 48 weeks postinfection (5).Few studies have investigated the course of recurrent bacteremia in naturally infected cats because follow-up investigations are diffi cult to conduct (1,6-9). Koehler et al.(1) detected recurrent bacteremia with a duration >2 months in 3 cats of patients with bacillary angiomatosis. Kordick et al. (6) reported positive blood cultures in cats of several CSD patients up to 14 months after collection of the initial positive culture. In the latter study, the fi rst blood culture was collected from the index cat of 1 CSD patient 22 months after the onset of the disease in the patient and contained B. henselae (7). Sander et al. (8) found repeated bacteremia in the cat of a CSD patient after 5 months and in 2 other cats after 1 year. In another study, B. henselae was isolated from the blood culture of a cat of a patient who had an episode 2.5 years earlier of debilitating fatigue with a duration of 1 month and without fever or lymphadenopathy (7). B. henselae was isolated again from the blood culture of the index cat after 5 months (7).In these studies, the question whether the cats were still infected by the initial B. henselae strain or had acquired a new strain was not addressed. It was concluded that the cats were persistently infected with B. henselae (8). We have recently demonstrated the appropriateness of pulsed-fi eld gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for differentiation of B. henselae isolates to the strain level (10,11). Therefore, we analyzed the clonal relationship between sequential B. henselae isolates that were obtained at different times from the blood of 2 cats to determine whether recurrences were caused by the initial strain or a new strain. The StudyWe tested 4 isolates of B. henselae: FR96/BK36...

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Emergence of distinct genetic variants in the population of primary Bartonella henselae isolates

Cited by 17 publications

References 39 publications

Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection withBartonella henselaeandBartonella vinsoniisubsp.berkhoffii

Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection withBartonella henselaeandBartonella vinsoniisubsp.berkhoffii

Bartonella henselae exists as a mosaic of different genetic variants in the infected host

ProlongedBartonella henselaeBacteremia Caused by Reinfection in Cats

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