Emergence of Klebsiella pneumoniae and Enterobacter cloacae producing OXA-48 carbapenemases from retail meats in China, 2018

Zhuang, Zilin; Lv, Luchao; Lu, Jiaxun; Lin, Jinhang; Liu, Jianhua

doi:10.1093/jac/dkz394

Cited by 6 publications

(1 citation statement)

References 10 publications

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“…Plasmids encoding antibiotic resistance in Enterobacterales belong to many different incompatibility groups, including IncA/C, IncI1, IncF, and IncX3 types that have been identified either from human or animal sources [25][26][27][28][29][30]. IncL plasmids are more often associated with human E. coli isolates but are increasingly reported from companion animals [31,32] and in the food chain [33][34][35]. Some of these plasmids are responsible for the global spread of clinically relevant Ambler class A extended-spectrum ß-lactamases (ESBLs) (e.g., CTX-Ms), class B (NDM-, IMP-, VIM-like), class C (AmpCs; e.g., CMY-like), or class D (e.g., OXA-48) ß-lactamases [25,26].…”

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confidence: 99%

Effect of Zinc Oxide and Copper Sulfate on Antibiotic Resistance Plasmid Transfer in Escherichia coli

Raro,

Poirel,

Nordmann

2023

Microorganisms

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Heavy metals such as zinc (Zn) and copper (Cu) may be associated with antibiotic resistance dissemination. Our aim was to investigate whether sub-lethal dosage of Zn and Cu may enhance plasmid transfer and subsequently resistance genes dissemination. Plasmid conjugation frequencies (PCF) were performed with Escherichia coli strains bearing IncL-blaOXA-48, IncA/C-blaCMY-2, IncI1-blaCTX-M-1, IncF-blaCTX-M-1, and IncX3-blaNDM-5 as donors. Mating-out assays were performed with sub-dosages of zinc oxide (ZnO) and Cu sulfate (CuSO4). Quantification of the SOS response-associated gene expression levels and of the production of reactive oxygen species were determined. Increased PCF was observed for IncL, IncA/C, and IncX3 when treated with ZnO. PCF was only increased for IncL when treated with CuSO4. The ROS production presented an overall positive correlation with PCF after treatment with ZnO for IncL, IncA/C, and IncX3. For CuSO4 treatment, the same was observed only for IncL. No increase was observed for expression of SOS response-associated genes under CuSO4 treatment, and under ZnO treatment, we observed an increase in SOS response-associated genes only for IncX3. Our data showed that sub-dosages of ZnO and CuSO4 could significantly enhance PCF in E. coli, with a more marked effect observed with IncL, IncA/C, and IncX3 scaffolds. Our study suggested that use of certain heavy metals is not the panacea for avoiding use of antibiotics in order to prevent the dissemination of antibiotic resistance.

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