Emergence of phenotypic and genotypic resistance in the intestinal microbiota of rainbow trout (Oncorhynchus mykiss) exposed long-term to sub-inhibitory concentrations of sulfamethoxazole

Labitt, Rachael N.; Ren, Jennifer; Marquis, Hélène

doi:10.1007/s10646-021-02480-9

Cited by 3 publications

(3 citation statements)

References 51 publications

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“…Although the concentration is much lower than the stricter MRLs for SAs in animal products required by China and the EU [ 9 , 10 ], their accumulation effects cannot be neglected. Some researchers have confirmed that antibiotics can enter the food chain through ingestion by aquatic organisms with exposure experiments [ 50 ]. Moreover, some antibiotics have also been widely detected in fish (without exposure experiments) that live in aquatic environments (ocean, river) in China [ 51 , 52 ].…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

Wang

Ji³

et al. 2022

Molecules

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The residues and abuse of antibiotics have seriously endangered ecological balance and human health; meanwhile, antibiotics determination is very difficult because of their low levels and multiple categories in complicated matrices. Appropriate sample pretreatment is usually imperative to enrich (ultra)trace antibiotics and eliminate matrix interference prior to chromatographic analysis. Dispersive liquid-liquid microextraction (DLLME) has become an ideal pretreatment technique owing to its simplicity, effectiveness, low-consumption, etc. In this work, an ultrasonic-assisted DLLME (UA-DLLME) was developed for the simultaneous extraction of seven sulfonamides (SAs) antibiotics in environmental water and seafood samples coupled with HPLC-DAD determination. Several parameters affecting UA-DLLME efficiency were systematically optimized, and consequently the SAs were separated and detected within 14.5 min. The obtained limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.7–7.8 μg/L and 2.4–26.0 μg/L for three water samples (seawater, aquaculture wastewater and lake water) and two seafood samples (pomfrets and shrimps). High recoveries (80.0–116.0%) with low relative standard deviations (0.1–8.1%) were achieved for all the tested samples at three spiked levels. Notably, sulfadimethoxine was found at 24.49 μg/L in one seawater sample. The facile, robust and benign DLLME-HPLC method demonstrated promising perspectives for multiresidue analysis of antibiotics.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

Wang

Ji³

et al. 2022

Molecules

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“…Other factors could have been explored to further assess the vulnerability/tolerance of fish to chemical pollutants, such as individual sex, which was not tested in the present study (Petitjean et al, 2021), neurotoxicological markers (Beghin et al, 2021), DNA damage (Silva et al, 2021). Examination of alterations in the microbiota of exposed gudgeons could also be an interesting approach because the constant exposure to subinhibitory concentrations of antibiotics may induce dysbiosis (Labitt et al, 2021) and impact biotransformation efficiency (Tan et al, 2022). Other biological matrices (gills, livers, or plasma) or standardized species (Dreissenidae or Gammaridae) with better-known biotransformation rates could be considered.…”

Section: Toxicological Effects On Caged Fishmentioning

confidence: 99%

Contrasted Antibiotics and Pesticides Occurrence in Fish Exposed In Situ to Urban Effluents: A 20‐Day Caging Experiment

Marchand,

Petit,

Alliot

et al. 2024

Enviro Toxic and Chemistry

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Urban freshwater ecosystems receive a wide array of organic pollutants through wastewater treatment plant (WWTP) discharges and agricultural runoff. Evaluating the fate and effects of antibiotics and pesticides can be a challenging task, especially the effects on freshwater vertebrates because of their abilities to metabolize and excrete these chemicals, and because of their high mobility and escape behavior when exposed to stressful environmental conditions. In the present study, 37 wild gudgeons (Gobio gobio) were caged for a period of up to 20 days, upstream and downstream of a WWTP effluent discharge in the Orge River (a tributary of the Seine River, France). The pesticides and antibiotics’ levels were weekly monitored in the fish muscles and compared to environmental contamination (water and sediments). Our results highlighted a slight impregnation of pesticides in the gudgeon muscles, at the downstream site, after 20 days of exposure. Concerning antibiotics, ofloxacin was the most detected compound in fish muscles (85% of occurrence) and ranged from undetectable to 8 ng.g‐1 dry weight. Antibiotic levels in fish muscle were not higher at the downstream site and did not increase with exposure duration, despite high levels in the water (up to 29 times greater than upstream). Potential ecotoxicological effects were also evaluated: body condition did not differ between the caging location and exposure time. Three oxidative status markers in the fish livers showed significant shifts after 14 days of caging. Our results suggest a high clearance rate of antibiotics and to a lesser extent, of pesticides in wild gudgeons, which could be explained by changes in xenobiotic metabolism with pollutant exposure.

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“…The term “sub-inhibitory”, which was originally described for pure cultures, is often applied to complex communities 16 – 20 . However, the members of a complex community differ in their response to antibiotics, with some members being inhibited and some members benefiting from the same antibiotic concentration 21 .…”

Section: Introductionmentioning

confidence: 99%

Sub-inhibitory gentamicin pollution induces gentamicin resistance gene integration in class 1 integrons in the environment

Sanchez-Cid

Ghaly

Gillings

et al. 2023

Sci Rep

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Antibiotics at sub-inhibitory concentrations are often found in the environment. Here they could impose selective pressure on bacteria, leading to the selection and dissemination of antibiotic resistance, despite being under the inhibitory threshold. The goal of this study was to evaluate the effects of sub-inhibitory concentrations of gentamicin on environmental class 1 integron cassettes in natural river microbial communities. Gentamicin at sub-inhibitory concentrations promoted the integration and selection of gentamicin resistance genes (GmRG) in class 1 integrons after only a one-day exposure. Therefore, sub-inhibitory concentrations of gentamicin induced integron rearrangements, increasing the mobilization potential of gentamicin resistance genes and potentially increasing their dissemination in the environment. This study demonstrates the effects of antibiotics at sub-inhibitory concentrations in the environment and supports concerns about antibiotics as emerging pollutants.

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Emergence of phenotypic and genotypic resistance in the intestinal microbiota of rainbow trout (Oncorhynchus mykiss) exposed long-term to sub-inhibitory concentrations of sulfamethoxazole

Abstract: Emergence of phenotypic and genotypic resistance in the intestinal microbiota of rainbow trout (Oncorhynchus mykiss) exposed long-term to sub-inhibitory concentrations of sulfamethoxazole

Cited by 3 publications

References 51 publications

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

Contrasted Antibiotics and Pesticides Occurrence in Fish Exposed In Situ to Urban Effluents: A 20‐Day Caging Experiment

Sub-inhibitory gentamicin pollution induces gentamicin resistance gene integration in class 1 integrons in the environment

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