Emerging roles of non-coding RNAs in the pathogenesis, diagnosis and prognosis of osteosarcoma

Wang, Chongchong; Jing, Juehua; Cheng, Li

doi:10.1007/s10637-018-0624-7

Cited by 53 publications

(47 citation statements)

References 180 publications

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“…It is necessary to find novel diagnostic, prognostic, and therapeutic targets for better management. Accumulating studies indicate that lncRNAs are involved in the progression of carcinogenesis for various malignancies, including OS (Smolle and Pichler, 2018;Wang et al, 2018). Therefore, lncRNAs may provide valuable biomarkers for diagnosis, prognosis, and therapy.…”

Section: Discussionmentioning

confidence: 99%

“…Long noncoding RNAs (LncRNAs) are a class of nonprotein-coding transcripts (larger than 200 bases) that participate in a variety of physiological and pathological functions (Wang et al, 2018). Increasing evidence suggests that lncRNAs play a critical role in cancer cell proliferation, migration, and apoptosis, and irregular lncRNAs levels are associated with malignancy initiation and development (Sun et al, 2018;de Oliveira et al, 2019;Flippot et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Long Noncoding RNA SERTAD2-3 Inhibits Osteosarcoma Proliferation and Migration by Competitively Binding miR-29c

Zhang

Liu

et al. 2020

Genetic Testing and Molecular Biomarkers

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Background: Osteosarcoma (OS) is a malignant tumor disease with high morbidity and mortality in children and adolescents. Recently, attention has been focused on the effects of long noncoding RNAs (lncRNAs) on tumor biology. In this study, we identified the role of lnc-SERTAD2-3 in the development of OS. Materials and Methods: Sixty OS samples and adjacent tissues were collected to determine the relationship between lnc-SERTAD2-3 levels and clinicopathological characteristics. Quantitative real-time PCR (qPCR) was used to measure gene expression levels. A transwell invasion assay, a Cell Counting Kit-8 assay, and flow cytometry were used to measure cell migration, growth, and apoptosis, respectively. The binding site between the lnc-SERTAD2-3 and miR-29c RNAs was evaluated using a luciferase reporter assay. Results: The expression of the lnc-SERTAD2-3 was significantly downregulated in OS samples and three OS cell lines (MG-63, U2OS, and Saos-2) compared to normal tissue. Patients with lower levels of lnc-SERTAD2-3 expression had a more unfavorable prognosis (larger OS size, distant metastasis, and recurrence). Overexpression of lnc-SERTAD2-3 inhibited proliferation and migration, and promoted apoptosis in OS cells. Moreover, we found that lnc-SERTAD2-3 could suppress miR-29c by direct binding. Moreover, reexpression of miR-29c reversed the effect of lnc-SERTAD2-3 on OS cells. Conclusion: Overall, lnc-SERTAD2-3, an OS suppressor, is involved in the inhibition of OS proliferation and migration by targeting miR-29c.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA SERTAD2-3 Inhibits Osteosarcoma Proliferation and Migration by Competitively Binding miR-29c

Zhang

Liu

et al. 2020

Genetic Testing and Molecular Biomarkers

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“…In addition, circRNAs are involved in various cancer types and exhibit multiple effects in regulating carcinogenesis and progression 5 . However, the mechanistic and functional characterization of circRNAs in osteosarcoma is largely unknown 6 .…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ_001621 promotes osteosarcoma cells proliferation and migration by sponging miR-578 and regulating VEGF expression

Shan

Shen

et al. 2020

Cell Death Dis

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Strategies targeted vascular endothelial growth factor (VEGF)-dependent osteosarcoma progression are limited although important progress has been made in illustrating the mechanisms. Here we identified circ_001621 as one of the significantly upregulated circular RNAs (circRNAs) by circRNAs microarrays. We found that patients with high circ_001621 expression had a shorter survival time. Moreover, we found several potential sponge micro RNAs (miRNA) of circ_001621 with Circular RNA Interactome database. Among the candidate sponge, we elucidated the association of circ_001621 and miR-578. In addition, we demonstrated that miR-578 targeted circ_001621 directly. Functionally, we set up the experimental system to investigate the effects of circ_001621/miR-578/VEGF interaction in vitro and in vivo. Results indicated circ_001621-promoted osteosarcoma proliferation and migration via attenuating the inhibition of cyclin-dependent kinase 4 (CDK4) and matrix metallopeptidase 9 (MMP9) by miR-578, respectively. Nude mice experiment was further performed to estimate the promotion of metastasis by circ_001621. The present study evaluated the mechanisms underlying circ_001621 enhanced osteosarcoma progression and provided novel therapeutic targets for advanced osteosarcoma.

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“…Accumulating evidence have revealed that miRNAs regulate many oncogenes and tumour suppressors in cells, and lead to initiation and progression of cancer. 21 Recent years, several miRNAs have been identified as key regulators of OS progression and excellent predictors for clinical outcome of OS patients 22,23 Decreased expression of miR-876-5p has been observed in HNSCC, HCC, ESCC and lung cancer. 14,15 Moreover, miR-876-5p underexpression is associated with poor prognostic features and reduced survival of HCC patients.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐876‐5p inhibits cell proliferation, migration and invasion by targeting c‐Met in osteosarcoma

Xie

Xiao

Wang

et al. 2019

J Cellular Molecular Medi

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Recently, aberrant expression of miR‐876‐5p has been reported to participate in the progression of several human cancers. However, the expression and function of miR‐876‐5p in osteosarcoma (OS) are still unknown. Here, we found that the expression of miR‐876‐5p was significantly down‐regulated in OS tissues compared to para‐cancerous tissues. Clinical association analysis indicated that underexpression of miR‐876‐5p was positively correlated with advanced clinical stage and poor differentiation. More importantly, OS patients with low miR‐876‐5p level had a significant shorter overall survival compared to miR‐876‐5p high‐expressing patients. In addition, gain‐ and loss‐of‐function experiments demonstrated that miR‐876‐5p restoration suppressed whereas miR‐876‐5p knockdown promoted cell proliferation, migration and invasion in both U2OS and MG63 cells. In vivo studies revealed that miR‐876‐5p overexpression inhibited tumour growth of OS in mice. Mechanistically, miR‐876‐5p reduced c‐Met abundance in OS cells and inversely correlated c‐Met expression in OS tissues. Herein, c‐Met was recognized as a direct target of miR‐876‐5p using luciferase reporter assay. Notably, c‐Met restoration rescued miR‐876‐5p attenuated the proliferation, migration and invasion of OS cells. In conclusion, these findings indicate that miR‐876‐5p may be used as a potential therapeutic target and promising biomarker for the diagnosis and prognosis of OS.

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Emerging roles of non-coding RNAs in the pathogenesis, diagnosis and prognosis of osteosarcoma

Cited by 53 publications

References 180 publications

Long Noncoding RNA SERTAD2-3 Inhibits Osteosarcoma Proliferation and Migration by Competitively Binding miR-29c

Long Noncoding RNA SERTAD2-3 Inhibits Osteosarcoma Proliferation and Migration by Competitively Binding miR-29c

Circular RNA circ_001621 promotes osteosarcoma cells proliferation and migration by sponging miR-578 and regulating VEGF expression

MicroRNA‐876‐5p inhibits cell proliferation, migration and invasion by targeting c‐Met in osteosarcoma

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