The introduction of a closely stacked quantum dot (QD) structure is considered as one of the most effective approaches to extend the emission wavelength of InAs/GaAs QDs beyond 1.3 μm (1300 nm). Herein, a trilayer QD structure (three closely stacked QD layers) is proposed to further extend the emission wavelength of QDs. Room‐temperature (RT) emission at 1418 nm from InAs/GaAs trilayer QDs is demonstrated. Moreover, based on these results, an E‐band InAs/GaAs trilayer QD laser is fabricated on a GaAs substrate, achieving RT oscillation at 1370 nm with a low threshold current density of 96 A cm−2 under continuous‐wave mode operation. The results indicate that the trilayer QD structure is promising for realizing high‐performance QD lasers on GaAs substrate over 1300 nm without incorporating metamorphic buffer layers.