Enantiomeric separation of organophosphorus pesticides by capillary electrophoresis

García-Ruiz, Carmen; Álvarez-Llamas, Gloria; Puerta, Angel de la; Blanco, Elisa; Sanz‐Medel, Alfredo; Marina, M. L.

doi:10.1016/j.aca.2005.04.027

Cited by 68 publications

(34 citation statements)

References 20 publications

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“…SPE was only employed as sample treatment to isolate chiral drugs in biological samples as urine [12,13,15,17] and to selectively preconcentrate chiral herbicides in spiked water samples [14,16]. Most of these works were performed with UV detection [12][13][14]17] achieving LODs in the 10 28 -10 27 M range. These LODs were improved by one or two orders of magnitude using SPE as sample treatment depending on the sample volume available (preconcentration factors ranging from 3 to 250 were achieved).…”

Section: Off-line Sample Treatment Techniques In Sensitive Chiral Anamentioning

confidence: 99%

Sensitive chiral analysis by CE: An update

et al. 2007

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Sensitive chiral analysis by CE: An updateA general view of the different strategies used in the last years to enhance the detection sensitivity in chiral analysis by CE is provided in this article. With this purpose and in order to update the previous review by García-Ruiz et al., the articles appeared on this subject from January 2005 to March 2007 are considered. Three were the main strategies employed to increase the detection sensitivity in chiral analysis by CE: (i) the use of off-line sample treatment techniques, (ii) the employment of in-capillary preconcentration techniques based on electrophoretic principles, and (iii) the use of alternative detection systems to the widely employed on-column UV-Vis absorption detection. Combinations of two or three of the above-mentioned strategies gave rise to adequate concentration detection limits up to 10 210 M enabling enantiomer analysis in a variety of real samples including complex biological matrices.

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Section: Off-line Sample Treatment Techniques In Sensitive Chiral Anamentioning

confidence: 99%

Sensitive chiral analysis by CE: An update

et al. 2007

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“…Typically, the extraction of drugs is performed using protein precipitation by organic solvents, [8][9][10] liq- uid-liquid extraction 11,12 and off-line SPE. [13][14][15][16] However, these conventional procedures are tedious, complicated and drugs may be partly lost in the sample preparation steps. Recently, solid-phase microextraction (SPME) has gained increasingly wide acceptance as a simple, fast, reliable, and flexible sample preparation technique, and it has been successfully applied to the method development for the determination of a variety of analytes from biological samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Caffeine and Theophylline in Human Plasma with a Weak Cation Monolithic SPE‐column

Zhu

Row²

2010

Chin. J. Chem.

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A simple reversed-phase high performance liquid chromatography (RP-HPLC) method was developed to determine the level of caffeine and theophylline in human plasma samples. The sample clean-up step involved the on-line solid-phase extraction (SPE) of the analytes from plasma samples into a weak cation monolithic column using a column switching system. Separation was performed on a C 18 column (5 µm, 150 mm×4.6 mm) with ultraviolet detection at 274 nm. The mobile phase consisted of methanol-water (32/68, V/V) under isocratic conditions at a flow rate of 0.6 mL•min -1 . The measured concentration of caffeine and theophylline showed a good linear relationship over the concentrations range, 0.1-80.0 µg•mL -1 . The absolute recoveries ranged from 77.10% to 85.39%, and the inter-day and intra-day relative standard deviations (RSD) were all less than 5%. This method avoids a tedious pretreatment and provides an economic, repeatable and effective method for assaying trace drugs in biological samples.

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“…using different anionic cyclodextrins as chiral selectors. 82) Normal-phase HPLC methods were employed for direct enantiomeric resolutions of chiral triazole pesticides by high-performance liquid chromatography. 83) Capillary electrophoresis has high potential for the enantioseparation of agrochemicals in real samples.…”

Section: Chiral Separationsmentioning

confidence: 99%

Chiral pesticides

Sekhon

2009

Journal of Pesticide Science

108

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The enantiomers of chiral pesticides are often metabolized at different rates. In agriculture, the preferred chiral form of a pesticide is more effective at lower application rates or more specific toward a targeted pest. Other advantages of chiral pure pesticides include greater environmental safety, reduced cost, greater specificity and extended patent life. Recent progress in optical resolution, asymmetric synthesis and biocatalysis of chiral pesticides has been described. Techniques to separate enantiomers with chiral HPLC and GC columns, electrophoresis and mass spectrometry have been reported. Chiral chase is actively pursuing asymmetric hydrogenation for the manufacture of a pure single-enantiomer pesticide. Enantioselectivity occurs in pesticidal activities toward target organisms as well as non-targeted organisms. There is a need to characterize both enantiomers of chiral pesticides in order to have an accurate understanding of their distribution and fate in the environment. Enantiomeric analysis can be useful in this aim and this is an important consideration in the risk assessment of pesticides. Use of only the target-active enantiomer of pesticides should be encouraged as it will reduce the pollutant load, provided it has no adverse impact on non-target organisms.

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Enantiomeric separation of organophosphorus pesticides by capillary electrophoresis

Cited by 68 publications

References 20 publications

Sensitive chiral analysis by CE: An update

Sensitive chiral analysis by CE: An update

Simultaneous Determination of Caffeine and Theophylline in Human Plasma with a Weak Cation Monolithic SPE‐column

Chiral pesticides

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