Encapsulation of bacteriophage T4 in mannitol-alginate dry macrospheres and survival in simulated gastrointestinal conditions

Śliwka, Paulina; Mituła, Paweł; Mituła, Agnieszka; Skaradziński, Grzegorz; Choińska-Pulit, Anna; Niezgoda, Natalia; Weber‐Dąbrowska, Beata; Żaczek, Maciej; Skaradzińska, Aneta

doi:10.1016/j.lwt.2018.09.043

Cited by 17 publications

(14 citation statements)

References 23 publications

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“…In another study, the mean encapsulation efficiency was determined as 93.3% for phage macroencapsules produced with 2.2% ALG–chitosan mixture as the encapsulating material B ( Ma et al, 2008 ). Śliwka et al (2019) microencapsulated phage T4 in a 2% alginate-mannitol mixture solution and reported the mean encapsulation efficiency, which was determined as 98%.…”

Section: Discussionmentioning

confidence: 99%

“…However, studies have shown that microcapsules produced by using only alginate could not protect phages against high acidity so that alginate is used in combination with various polymers such as chitosan, pectin, mannitol, whey protein, and gelled milk ( Samtlebe et al, 2016 ; Vinner and Malik, 2018 ; Śliwka et al, 2019 ). It was reported that alginate and κ-carrageenan (CG) formed spherical and uniformly shaped composite microcapsules containing a biopolymer network held together by calcium ions.…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of Salmonella phage SL01 in alginate/carrageenan microcapsules as a delivery system and its application in vitro

Zhou

et al. 2022

Front. Microbiol.

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Phages can be used successfully to treat pathogenic bacteria including zoonotic pathogens that colonize the intestines of animals and humans. However, low pH and digestive enzyme activity under harsh gastric conditions affect phage viability, thereby reducing their effectiveness. In this study, alginate (ALG)/κ-carrageenan (CG) microcapsules were developed to encapsulate and release phage under simulated gastrointestinal conditions. The effects of ALG and CG concentrations on the encapsulation and loading efficiency of microcapsules, as well as the release behavior and antibacterial effects of microcapsules in simulating human intestinal pH and temperature, were investigated. Based on various indicators, when the concentration of ALG and CG were 2.0 and 0.3%, respectively, the obtained microcapsules have high encapsulation efficiency, strong protection, and high release efficiency in simulated intestinal fluid. This effect is attributed to the formation of a more tightly packed biopolymer network within the composite microcapsules based on the measurements of their microstructure properties. Bead-encapsulation is a promising, reliable, and cost-effective method for the functional delivery of phage targeting intestinal bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Encapsulation of Salmonella phage SL01 in alginate/carrageenan microcapsules as a delivery system and its application in vitro

Zhou

et al. 2022

Front. Microbiol.

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“…Techniques like emulsification, freeze drying, spray drying, encapsulation in liposomes, and electrospinning, in which bacteriophages are coated/surrounded by certain stabilizing agents, protect the virions against the external environment and have been used in phage encapsulation [ 66 , 79 ]. Śliwka et al [ 80 ] developed microspheres of mannitol–alginate with the aim to obtain a dry powder phage preparation. To limit the adverse effects of the drying process, it is possible to apply different protective additives during phage encapsulation such as mannitol, trehalose, maltodextrin, sucrose, and skim milk, among others.…”

Section: Phage Delivery Strategies For Human Health Applicationsmentioning

confidence: 99%

Phage Delivery Strategies for Biocontrolling Human, Animal, and Plant Bacterial Infections: State of the Art

Vila,

Balcão,

Balcão

2024

Pharmaceutics

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This review aims at presenting the main strategies that are currently available for the delivery of bacteriophages to combat bacterial infections in humans, animals, and plants. It can be seen that the main routes for phage delivery are topical, oral, systemic, and airways for humans. In animals, the topical and oral routes are the most used. To combat infections in plant species, spraying the plant’s phyllosphere or drenching the soil are the most commonly used methods. In both phage therapy and biocontrol using phages, very promising results have been obtained so far. However, more experiments are needed to establish forms of treatment and phage doses, among other parameters. Furthermore, in general, there is a lack of specific standards for the use of phages to combat bacterial infections.

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“…The drug resistance and legislative restrictions on the use of antibiotics in livestock farming in the countries of the European Union, [6,7,8] and in the USA [1] make it necessary to develop new solutions in the elimination of pathogens. In recent years a number of scientists and clinicians are looking again at application of bacteriophages as a therapeutic option in the treatment of bacterial pathogens infections that may contaminate the food supply [9,10]. Bacteriophages are viruses that infect bacteria.…”

Section: Introductionmentioning

confidence: 99%

Low-temperature drying: experimental viability analysis of bacteriophages (Siphoviridae family) immobilized in alginate substrate

Wojciech¹,

Magdalena²,

Monika³

et al.

Proceedings of the 22nd International Drying Symposium on Drying Technology - IDS '22

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Supplementing animal food with bacteriophages active against animal bacterial pathogens can be an excellent way of mildly influencing the quality of food and the health condition of animal breeding. Polysaccharides were used to immobilize biologically active cultures, and the technique of low-temperature drying under atmospheric pressure was chosen to maintain microbiological activity. Periodically dried material had the form of spheres with initial diameter 3 mm and moisture content 97%. The average initial concentration of bacteriophages of Siphoviridae family was 1×10 8 PFU/g (plaque-forming units per gram). Drying tests were carried out in temperatures range from -10°C up to 30°C. The activity of bacteriophages was checked prior and after drying, by enumeration by double agar overlay plaque assay.

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Encapsulation of bacteriophage T4 in mannitol-alginate dry macrospheres and survival in simulated gastrointestinal conditions

Cited by 17 publications

References 23 publications

Encapsulation of Salmonella phage SL01 in alginate/carrageenan microcapsules as a delivery system and its application in vitro

Encapsulation of Salmonella phage SL01 in alginate/carrageenan microcapsules as a delivery system and its application in vitro

Phage Delivery Strategies for Biocontrolling Human, Animal, and Plant Bacterial Infections: State of the Art

Low-temperature drying: experimental viability analysis of bacteriophages (Siphoviridae family) immobilized in alginate substrate

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