Encapsulation of Salmonella phage SL01 in alginate/carrageenan microcapsules as a delivery system and its application in vitro

Zhou, Yuqiao; Xu, Dong; Yu, Hongfang; Han, Jianzhong; Li, Weilin; Qu, Daofeng

doi:10.3389/fmicb.2022.906103

Cited by 9 publications

(10 citation statements)

References 36 publications

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“…As pH values reach 5.5 and above, the alginic acid dissolves and the alginate disassociates [10,16]. Previous studies have exploited this pH-dependent solubility of pure alginate, and modified alginate gels like those formulated in this study to demonstrate the successful survival of phages targeting pathogenic bacteria in simulated gastrointestinal environments [13][14][15]. For this reason, this study focused on the alginate encapsulation of LOCARD, and is the first study to describe such an encapsulation approach for MPs.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that phages suspended in alginate matrices-and subsequently encapsulated in alginate gels-could be shielded from the acidic environment of the stomach, and later released into the intestinal environment as the pH gradually increases along the small intestine [11]. This was successfully demonstrated with various formulations of alginate-encapsulated phages (i.e., often containing additional gelling agents, e.g., chitosan [12]) in simulated gastrointestinal environments using phages targeting Escherichia coli, Salmonella enterica, and Listeria monocytogenes [13][14][15]. This study aims to demonstrate similar outcomes with MPs.…”

Section: Introductionmentioning

confidence: 99%

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Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

O’Connell,

Coffey,

O’Mahony

2023

Viruses

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Encapsulated medication is a common method of administering therapeutic treatments. As researchers explore alternative therapies, it is likely that encapsulation will remain a feature of these novel treatments, particularly when routes of delivery are considered. For instance, alginate-encapsulation is often favoured where gastric digestion poses an obstacle. When exposed to cations (namely Ca2+), alginate readily forms gels that are resilient to acidic conditions and readily dissociate in response to mid-range pH. This action can be extremely valuable for the encapsulation of phages. The efficient delivery of phages to the intestine is important when considering mycobacteriophage (MP) therapy (or MP prophylaxis) for disseminated mycobacterial infections and chronic gastroenteritis conditions. This study presents the design and in vitro validation of an alginate-encapsulated MP capable of releasing phages in a pH-dependent manner. Ultimately, it is shown that encapsulated phages pretreated with simulated gastric fluid (SGF) are capable of releasing viable phages into simulated intestinal fluid (SIF) and thereby reducing the mycobacterial numbers in spiked SIF by 90%. These findings suggest that alginate encapsulation may be a viable option for therapeutic and prophylactic approaches to the management of intestinal mycobacterial disease, such as Johne’s disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

O’Connell,

Coffey,

O’Mahony

2023

Viruses

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“…Iota-carrageenan is a sulfate hydrocolloid that is widely used in pharmacological, medicinal, and non-food applications. It is derived from seaweed [ 48 ].…”

Section: Natural Polymers Used For Encapsulationmentioning

confidence: 99%

Natural Polymers as Carriers for Encapsulation of Volatile Oils: Applications and Perspectives in Food Products

Tița,

Constantinescu,

Rusu

et al. 2024

Polymers

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The technique of encapsulating different materials into matrices that can both protect and release their contents under specific circumstances is known as encapsulation. It serves the primary function of shielding delicate components from outside influences, including heat, light, and humidity. This can be accomplished by a variety of procedures that, depending on the method and materials selected, result in the creation of particles with various structures. The materials used for encapsulation in food applications must be of high quality, acceptable for human consumption, and stable during processing and storage. The most suitable natural polymers for food applications are carbohydrates, proteins, or mixtures thereof. Volatile oils are end products of plant metabolism, accumulated and stored in various plant organs, cells, or secretory tissues. These are natural and are characterized by the scent of the aromatic plants they come from. Because of their antibacterial and antioxidant qualities, they are being utilized more and more in the food and pharmaceutical industries. Since volatile oils are highly sensitive to environmental changes, they must be stored under specific conditions after being extracted from a variety of plant sources. A promising method for increasing the applicability of volatile oils is their encapsulation into colloidal particles by natural polymers such as carbohydrates and proteins. Encapsulation hides the unfavorable taste of nutrients while shielding delicate dietary ingredients from the effects of heat, moisture, oxygen, and pH. This technique results in improved stability for volatile oils that are often sensitive to environmental factors and offers the possibility of using them in an aqueous system even if they are insoluble in water. This paper aims to provide an overview of the current advances in volatile oil encapsulation technologies and presents a variety of natural polymers used in the food industry for encapsulation. Also, a distinct section is created to highlight the current advances in dairy products enriched with encapsulated volatile oils.

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“…The combination of alginate and carrageenan improved phage protection against acidic conditions. In general, the composite microcapsules significantly enhanced phage viability in the gastric environment, although different ratios of alginate and κ-carrageenan showed inconsistent protective efficacy ( Figure 18 ) [ 109 ].…”

Section: Wall Materials: Natural Polymer Coatingsmentioning

confidence: 99%

Encapsulation of Active Substances in Natural Polymer Coatings

Akpo,

Colin,

Perrin

et al. 2024

Materials

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Already used in the food, pharmaceutical, cosmetic, and agrochemical industries, encapsulation is a strategy used to protect active ingredients from external degradation factors and to control their release kinetics. Various encapsulation techniques have been studied, both to optimise the level of protection with respect to the nature of the aggressor and to favour a release mechanism between diffusion of the active compounds and degradation of the barrier material. Biopolymers are of particular interest as wall materials because of their biocompatibility, biodegradability, and non-toxicity. By forming a stable hydrogel around the drug, they provide a ‘smart’ barrier whose behaviour can change in response to environmental conditions. After a comprehensive description of the concept of encapsulation and the main technologies used to achieve encapsulation, including micro- and nano-gels, the mechanisms of controlled release of active compounds are presented. A panorama of natural polymers as wall materials is then presented, highlighting the main results associated with each polymer and attempting to identify the most cost-effective and suitable methods in terms of the encapsulated drug.

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Encapsulation of Salmonella phage SL01 in alginate/carrageenan microcapsules as a delivery system and its application in vitro

Cited by 9 publications

References 36 publications

Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

Alginate-Encapsulated Mycobacteriophage: A Potential Approach for the Management of Intestinal Mycobacterial Disease

Natural Polymers as Carriers for Encapsulation of Volatile Oils: Applications and Perspectives in Food Products

Encapsulation of Active Substances in Natural Polymer Coatings

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