Encapsulation of CdSe/ZnS quantum dots in poly(ethylene glycol)-poly(D,L-lactide) micelle for biomedical imaging and detection

Lee, Yong Kyu; Hong, Suk Min; Kim, Jin Su; Im, Jeong Hyuk; Min, Hyun Su; Subramanyam, Elango; Huh, Kang Moo; Park, Sung Woo

doi:10.1007/bf03218795

Cited by 33 publications

(14 citation statements)

References 29 publications

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“…Whereas the vast majority of synthetic procedures for micelle formation involve heating and evaporation of an organic solvent 39,41,43,44,50 or dissolution of an organic film in aqueous media or a similar method of phase transfer, 40,42,45,46,49,51,52 sonication offers an alternative method to assemble micelles. In contrast to other protocols, 47 we were able to rapidly (~5 min) and facilely produce micelle nanosensors of a consistent size by conducting sonication at room temperature.…”

Section: Resultsmentioning

confidence: 99%

Micelle-Encapsulated Quantum Dot-Porphyrin Assemblies as in Vivo Two-Photon Oxygen Sensors

et al. 2015

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Micelles have been employed to encapsulate the supramolecular assembly of quantum dots with palladium(II) porphyrins for the quantification of O2 levels in aqueous media and in vivo. Förster resonance energy transfer from the quantum dot (QD) to the palladium porphyrin provides a means for signal transduction under both one- and two-photon excitation. The palladium porphyrins are sensitive to O2 concentrations in the range of 0–160 Torr. The micelle-encapsulated QD-porphyrin assemblies have been employed for in vivo multiphoton imaging and lifetime-based oxygen measurements in mice with chronic dorsal skinfold chambers or cranial windows. Our results establish the utility of the QD-micelle approach for in vivo biological sensing applications.

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Section: Resultsmentioning

confidence: 99%

Micelle-Encapsulated Quantum Dot-Porphyrin Assemblies as in Vivo Two-Photon Oxygen Sensors

et al. 2015

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“…For instance, cellular internalization and intracellular trafficking of fluorescent NPs, as well as in vivo biodistribution of fluorescent NPs, can be conveniently carried out using fluorescence microscopy[21–24]. Current strategies to create fluorescent NPs include conjugating or encapsulating organic dyes or utilizing inorganic fluorescent NPs such as quantum dots (QDs) or other metallic particles[25–27]. However, there are various limitations to these common approaches.…”

Section: Introductionmentioning

confidence: 99%

Multi-functional self-fluorescent unimolecular micelles for tumor-targeted drug delivery and bioimaging

et al. 2015

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A novel type of self-fluorescent unimolecular micelle nanoparticle (NP) formed by multi-arm star amphiphilic block copolymer, Boltron® H40 (H40, a 4th generation hyperbranched polymer)-biodegradable photo-luminescent polymer (BPLP)-poly(ethylene glycol) (PEG) conjugated with cRGD peptide (i.e., H40-BPLP-PEG-cRGD) was designed, synthesized, and characterized. The hydrophobic BPLP segment was self-fluorescent, thereby making the unimolecular micelle NP self-fluorescent. cRGD peptides, which can effectively target αvβ3 integrin-expressing tumor neovasculature and tumor cells, were selectively conjugated onto the surface of the micelles to offer active tumor-targeting ability. This unique self-fluorescent unimolecular micelle exhibited excellent photostability and low cytotoxicity, making it an attractive bioimaging probe for NP tracking for a variety of microscopy techniques including fluorescent microscopy, confocal laser scanning microscopy (CLSM), and two-photon microscopy. Moreover, this self-fluorescent unimolecular micelle NP also demonstrated excellent stability in aqueous solutions due to its covalent nature, high drug loading level, pH-controlled drug release, and passive and active tumor-targeting abilities, thereby making it a promising nanoplatform for targeted cancer theranostics.

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“…This is because the DLS technique measures the mean hydrodynamic diameter of the GM core surrounded by the organic and solvation layers, and this hydrodynamic diameter is affected by the viscosity and concentration of the solution. TEM, on the other hand, measures the diameter of the core alone [25]. The immobilization of folic acid on the surface of GE was confirmed by electron spectroscopy for chemical analysis (ESCA), as shown in Figure 5.…”

Section: Surface Characterizationmentioning

confidence: 84%

Targeted images of KB cells using folate-conjugated gold nanoparticles

Rathinaraj¹,

Lee²,

Park³

et al. 2016

Nano Online

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Mercaptosuccinic acid-coated gold (GM) nanoparticles were prepared and characterized by transmission electron microscopy and dynamic light scattering. Folic acid (F) was then conjugated to the GM to preferentially target oral squamous cancer (KB) cells with folate receptors expressed on their membranes and facilitate the transit of the nanoparticles across the cell membrane. Finally, a fluorescence dye (Atto) was conjugated to the nanoparticles to visualize their internalization into KB cells. After culture of the cells in a medium containing GM and folateconjugated GM (GF), the interaction of surface-modified gold nanoparticles with KB cells was studied.

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Encapsulation of CdSe/ZnS quantum dots in poly(ethylene glycol)-poly(D,L-lactide) micelle for biomedical imaging and detection

Cited by 33 publications

References 29 publications

Micelle-Encapsulated Quantum Dot-Porphyrin Assemblies as in Vivo Two-Photon Oxygen Sensors

Micelle-Encapsulated Quantum Dot-Porphyrin Assemblies as in Vivo Two-Photon Oxygen Sensors

Multi-functional self-fluorescent unimolecular micelles for tumor-targeted drug delivery and bioimaging

Targeted images of KB cells using folate-conjugated gold nanoparticles

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