Encapsulation of docetaxel in oily core polyester nanocapsules intended for breast cancer therapy

Abstract

This study is designed to test the hypothesis that docetaxel [Doc] containing oily core nanocapsules [NCs] could be successfully prepared with a high percentage encapsulation efficiency [EE%] and high drug loading. The oily core NCs were generated according to the emulsion solvent diffusion method using neutral Labrafac CC and poly(d, l-lactide) [PLA] as oily core and shell, respectively. The engineered NCs were characterized for particle mean diameter, zeta potential, EE%, drug release kinetics, morphology, crystallinity, and cytotoxicity on the SUM 225 breast cancer cell line by dynamic light scattering, high performance liquid chromatography, electron microscopies, powder X-ray diffraction, and lactate dehydrogenase bioassay. Typically, the formation of Doc-loaded, oily core, polyester-based NCs was evidenced by spherical nanometric particles (115 to 582 nm) with a low polydispersity index (< 0.05), high EE% (65% to 93%), high drug loading (up to 68.3%), and a smooth surface. Powder X-ray diffraction analysis revealed that Doc was not present in a crystalline state because it was dissolved within the NCs' oily core and the PLA shell. The drug/polymer interaction has been indeed thermodynamically explained using the Flory-Huggins interaction parameters. Doc release kinetic data over 144 h fitted very well with the Higuchi model (R2 > 0.93), indicating that drug release occurred mainly by controlled diffusion. At the highest drug concentration (5 μM), the Doc-loaded oily core NCs (as a reservoir nanosystem) enhanced the native drug cytotoxicity. These data suggest that the oily core NCs are promising templates for controlled delivery of poorly water soluble chemotherapeutic agents, such as Doc.

Temperature‐sensing ink and system for quality management in distribution from production to consumption

“…Accordingly, when selecting the materials, the compatibility of the leuco dye, the color developer, and the decolorant is critical. As such, we used Hansen's solubility parameter (SP value) to predict the compatibility between each material, and selected the leuco dye, the color developer, and the decolorant for which compatibility was high 6–10 …”

“…As such, we used Hansen's solubility parameter (SP value) to predict the compatibility between each material, and selected the leuco dye, the color developer, and the decolorant for which compatibility was high. [6][7][8][9][10] Hansen's SP value is a value used to predict the solubility of a substance, and has the following three interaction parameters: energy from dispersion force ( d ), energy from dipolar intermolecular force ( p ), and energy from hydrogen bonds ( h ). Using these three parameters, the compatibility of two materials can be expressed as shown in Equation (1): In this equation, Δ is an index that represents compatibility.…”

Ultrastable Liquid–Liquid Interface as Viable Route for Controlled Deposition of Biodegradable Polymer Nanocapsules

“…There is an increasing interest toward liquid–liquid interfaces as templates for interfacial deposition demonstrated by recent reports, for example on powdered liquids, colloidosomes, and polymeric capsules . Additionally, most of the drugs limited by their poor solubility in water can be efficiently used preloading a lipophilic inner liquid template, thus avoiding time consuming postloading processing . Moreover, compared to solid–liquid interfaces, there are no risks of solid template residues and damaging of polymer shell due to harsh leaching out conditions,[6b], especially while using biomolecule based polymers.…”

“…Additionally, most of the drugs limited by their poor solubility in water can be efficiently used preloading a lipophilic inner liquid template, thus avoiding time consuming postloading processing . Moreover, compared to solid–liquid interfaces, there are no risks of solid template residues and damaging of polymer shell due to harsh leaching out conditions,[6b], especially while using biomolecule based polymers.…”

Developing Docetaxel-Loaded Nanocapsules: A Promising Approach for Cancer Therapy

“…Their lipid core allows the efficient encapsulation of lipophilic drugs (most anticancer drugs are lipophilic), whereas their polymeric shell may have the role of improving the stability of the oily droplets and facilitating their uptake by the cancer cells. Moreover, the presence of the polymer around the oily core offers the possibility of chemical modifications, which may further improve the stability, cellular uptake and biodistribution of NCs [15,[21][22] .…”