Encapsulation of honokiol into self-assembled pectin nanoparticles for drug delivery to HepG2 cells

Zhang, Yuxia; Chen, Tong; Yuan, Pei; Tian, Rui; Hu, Wenjing; Tang, Yali; Jia, Yuntao; Zhang, Liangke

doi:10.1016/j.carbpol.2015.06.102

Cited by 77 publications

(35 citation statements)

References 33 publications

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“…Ionotropic gelation and gel coating have been especially suggested to produce pectin‐based drug delivery carriers . Pectin nanoparticles are considered as hydrophilic gel nanoparticles which can efficiently encapsulate water‐soluble drugs . However, high hydrophilic nature of pectin‐based nanosystems may limit their ability to protect the loaded cargos during gastrointestinal tract .…”

Section: Polysaccharide Nanoparticlesmentioning

confidence: 99%

“…In contrast, uptake in A549 cells was not attributed to the ASGR, as the inclusion of free galactose did not show an inhibitory effect on the amount of uptake. Therefore, uptake in A549 cells may be referred to as the non‐specific binding of nanoparticles to the cell surface . Another study showed that cellular uptake could be increased when using pectin nanoparticles (Fig.…”

Section: Polysaccharide Nanoparticlesmentioning

confidence: 99%

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Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Salatin

Khosroushahi

2017

J Cellular Molecular Medi

246

143

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Nanoparticulate drug/gene carriers have gained much attention in the past decades because of their versatile and tunable properties. However, efficacy of the therapeutic agents can be further enhanced using naturally occurring materials‐based nanoparticles. Polysaccharides are an emerging class of biopolymers; therefore, they are generally considered to be safe, non‐toxic, biocompatible and biodegradable. Considering that the target of nanoparticle‐based therapeutic strategies is localization of biomedical agents in subcellular compartments, a detailed understanding of the cellular mechanism involved in the uptake of polysaccharide‐based nanoparticles is essential for safe and efficient therapeutic applications. Uptake of the nanoparticles by the cellular systems occurs with a process known as endocytosis and is influenced by the physicochemical characteristics of nanoparticles such as size, shape and surface chemistry as well as the employed experimental conditions. In this study, we highlight the main endocytosis mechanisms responsible for the cellular uptake of polysaccharide nanoparticles containing drug/gene.

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Section: Polysaccharide Nanoparticlesmentioning

confidence: 99%

Section: Polysaccharide Nanoparticlesmentioning

confidence: 99%

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Salatin

Khosroushahi

2017

J Cellular Molecular Medi

246

143

View full text Add to dashboard Cite

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“…For intravenous administration, HNK-FD was encapsulated in PEG-PCL-PEG [38]. In another study, loading of HNK into self assembled pectin nanoparticles was carried out to deliver to HepG2 cells and it was observed that the anticancer activity was increased in the nanoformulation group compared to free drug [39]. Though, nanoparticle based HNK formulations have been reported, most of the studies suggest that these formulations are suitable for intravenous administration.…”

Section: Discussionmentioning

confidence: 99%

Honokiol nanomicellar formulation produced increased oral bioavailability and anticancer effects in triple negative breast cancer (TNBC)

Godugu

Doddapaneni

Singh

2017

Colloids and Surfaces B: Biointerfaces

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Triple negative breast cancer (TNBC), owing to its aggressive behavior and toxicity associated with available chemotherapy; currently no suitable therapy is available. Honokiol (HNK) is a promising anticancer drug but has poor bioavailability. In the current study, we evaluated the anticancer effects of an oral Honokiol nanomicellar (NM) formulation (size range of 20–40 nm) in vitro against various TNBC cells lines. Cytotoxicity, clonogenic and wound healing assays demonstrated the promising anticancer effects. In vitro Caco-2 permeability studies suggested increased absorption of Honokiol. Compared to HNK-FD, nanomicellar formulations resulted in significant increase in the oral bioavailability. Cmax (4.06 and 3.60-fold) and AUC (6.26 and 5.83-fold) were significantly increased in comparison to oral 40 and 80 mg/kg free drug respectively. Further, anticancer effects of these formulations were studied in BALB/c nude mice transplanted with orthotopic MDA-MB-231 cell induced xenografts. After 4 weeks of daily administration of HNK-NM formulation, significant reduction in the tumor volumes and weights compared to free drug (p < 0.001) treated groups was observed. Surprisingly, in some of the animals (25%), the treatment resulted in complete eradication of tumors. Increased apoptosis and antiangiogenic effect was observed in HNK-NM groups compared to free drug and untreated control animals. This is the first report demonstrating that HNK-FD possesses anticancer effects against TNBC.

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“…PNs loaded with CPE (E‐PNs) were prepared using the ionic gelation technique (Zhang et al ., ) with some modification. The process of synthesis illustrated in Fig.…”

Section: Methodsmentioning

confidence: 99%

In vitro release and antioxidant activity of Satsuma mandarin (Citrus reticulata Blanco cv. unshiu) peel flavonoids encapsulated by pectin nanoparticles

Zhang

et al. 2017

Int J of Food Sci Tech

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The flavonoids of citrus peel extracts (CPE) were encapsulated by pectin nanoparticles (PNs), in an attempt to control the releasing property and improve their antioxidant activity. The physiochemical properties of the obtained PNs were characterised. Flavonoid composition in CPE before and after encapsulation were determined. In vitro release profiles and antioxidant activity of CPE were further evaluated. Results showed the size of spherical PNs obtained was 271.5 AE 5.3 nm. Interestingly, in simulated gastric fluid (SGF), 73% flavonoids were released from the naked CPE after 2 h, while only 28.78% from the CPE-loaded PNs (E-PNs). The releasing rate of flavonoids reached 91.47% from E-PNs after 24 h in the simulated intestinal fluid (SIF). Moreover, DPPH and ABTS assay revealed that E-PNs showed higher antioxidant activity than blank PNs and free CPE. These data showed that the controlled release of CPE might potentially increase its bioaccessibility and there was a synergistic effect between citrus PNs and CPE on the antioxidant activity. Encapsulation, release, antioxidant activity Y. Hu et al. Encapsulation, release, antioxidant activity Y. Hu et al.Figure 6 Comparison of antioxidant activity of CPE by DPPH• + (a) and ABTS• + (b) and antioxidant activity of E-PNs and pectin by DPPH• + (c) and ABTS• + (d) at different concentrations, data are reported as means AE SD, n = 3. Encapsulation, release, antioxidant activity Y. Hu et al.

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Encapsulation of honokiol into self-assembled pectin nanoparticles for drug delivery to HepG2 cells

Cited by 77 publications

References 33 publications

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Honokiol nanomicellar formulation produced increased oral bioavailability and anticancer effects in triple negative breast cancer (TNBC)

In vitro release and antioxidant activity of Satsuma mandarin (Citrus reticulata Blanco cv. unshiu) peel flavonoids encapsulated by pectin nanoparticles

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