Encapsulation of sex sorted boar semen: Sperm membrane status and oocyte penetration parameters

Spinaci, Marcella; Chlapanidas, Theodora; Bucci, Diego; Vallorani, Claudia; Perteghella, Sara; Lucconi, G.; Communod, Ricardo; Vigo, D.; Galeati, Giovanna; Faustini, M.; Torre, Maria Luisa

doi:10.1016/j.theriogenology.2012.10.021

Cited by 14 publications

(10 citation statements)

References 31 publications

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“…Some of these obstacles could be overcome by adopting a sperm encapsulation technique, which was previously demonstrated in bovine [13171836] and swine [78232930313334] species with promising results. Alginate encapsulation of sperm can limit damage to spermatozoa and allow prolonged sperm release; moreover, the bioadhesiveness of alginate can prevent semen backflow.…”

Section: Introductionmentioning

confidence: 99%

Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation

et al. 2017

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The use of artificial insemination (AI) in buffalo (Bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. Although AI procedures are commonly used in bovine, use of AI has been limited in buffalo. In the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability of buffalo spermatozoa after AI. In this study, for the first time, the use of alginate encapsulation and cryopreservation of buffalo spermatozoa is described, and the same procedure was performed with Holstein Friesian (Bos taurus) semen. Results obtained from in vitro analyses indicate that the encapsulation process does not have detrimental effects (compared to controls) on quality parameters (membrane integrity, progressive motility, path average velocity) in either species. Similarly, there were no detrimental effects after cryopreservation in either species. The fertilizing potential of encapsulated and cryopreserved semen was evaluated after AI in 25 buffalo and 113 bovine females. Pregnancy rates were not affected in either species. The results of this study show proof of concept for the use of frozen semen controlled-release devices in buffalo.

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Section: Introductionmentioning

confidence: 99%

Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation

et al. 2017

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“…The IVF assays performed after 24, 48, and 72 hours of storage at 15 C to assess the impact of the encapsulation process on the fertilizing potential of sorted spermatozoa confirmed the progressive and time-dependent reduction of the fertilizing ability of flow cytometrically sorted spermatozoa [85]. Although the combination of sorting and encapsulation techniques did not lead to a reduction of membrane integrity (plasmalemma and acrosome), a decrease in penetration rates was observed after storage.…”

Section: Encapsulation Of Sex-sorted Boar Spermatozoamentioning

confidence: 67%

“…The results obtained reported that the encapsulation technique does not damage sorted boar semen. During 72 hours of storage, no differences were observed between diluted and encapsulated sorted spermatozoa in terms of membrane and acrosome integrity [85]. Although the sorting procedure reduced sperm viability, encapsulation limited the damage to sorted spermatozoa.…”

Section: Encapsulation Of Sex-sorted Boar Spermatozoamentioning

confidence: 88%

Storage of sexed boar spermatozoa: Limits and perspectives

et al. 2016

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Despite the great potential application of sex-sorted spermatozoa in swine, the technology is not practiced in the pig industry because of technical factors and species-specific issues. The susceptibility of boar spermatozoa to stresses induced by the sorting procedure, the relative slowness of the sex-sorting process together with the high sperm numbers required for routine artificial insemination in pig are some of the main factors limiting the commercial application of this technology in pigs. This review briefly describes the damage to spermatozoa during sex sorting, focusing on an additional limiting factor: increased susceptibility of sexed boar spermatozoa to injuries induced by liquid storage and cryopreservation that, in turn, impairs sperm quality leading to unsatisfactory results in vivo. Strategies to extend the lifespan of sex-sorted boar spermatozoa and to improve their fertilizing ability after liquid storage or cryopreservation need to be implemented before this technology can be used in pig farms. In this regard, encapsulation in barium alginate membranes could be a promising technique to optimize the in vivo use of sexed boar spermatozoa, by protecting, targeting, and controlling the release of sperm into the female genital tract.

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“…We investigated the effect of encapsulation technology on plasma membrane and acrosomal integrity of pig sexed sperm cells during liquid storage at 15°C for 72 h (Spinaci et al. ). The results showed that the encapsulation process did not produce a significant supplemental injury to the membranes during storage.…”

Section: Sex Sorting and Sperm Encapsulation: New Challenges In Boar mentioning

confidence: 99%

“…For this reason, we performed an in vitro fertilization assay after 24, 48 and 72 h of storage of both diluted and encapsulated sorted semen (Spinaci et al. ). The percentage of penetrated oocytes was lower in the capsule group (approximately 25%) with respect to diluted semen (approximately 47%).…”

Section: Sex Sorting and Sperm Encapsulation: New Challenges In Boar mentioning

confidence: 99%

Sperm Encapsulation from 1985 to Date: Technology Evolution and New Challenges in Swine Reproduction

Perteghella

Vigani

Crivelli

et al. 2015

Reprod Domestic Animals

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Contents In the last 30 years, encapsulation technology has been applied to different species to minimize the loss of spermatozoa after artificial insemination. In particular, the vehiculation of boar sperm cells in barium alginate membrane has proved a valid strategy to reduce the risk of polyspermy and optimize in vivo fertilizing yields. Controlled release of male gametes into the female genital tract has reduced the minimum fertilizing dose of spermatozoa. Notwithstanding these results, encapsulation has not yet reached commercial application, largely due to the additional costs of production. However, encapsulation could be useful in advanced reproductive technology, such as sex sorting, to store sorted boar semen. The controlled release of flow cytometrically sorted spermatozoa could be a promising strategy to reduce the number of cells necessary for each insemination and hence allow the widescale use of sex sorting in this species.

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Encapsulation of sex sorted boar semen: Sperm membrane status and oocyte penetration parameters

Cited by 14 publications

References 31 publications

Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation

Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation

Storage of sexed boar spermatozoa: Limits and perspectives

Sperm Encapsulation from 1985 to Date: Technology Evolution and New Challenges in Swine Reproduction

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