Encapsulation of single cells into monodisperse droplets by fluorescence-activated droplet formation on a microfluidic chip

Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

“…4,5 Furthermore, undesirable reactions and cross-contaminations between samples can be prevented to gain increased sensitivity. To date, microfluidic droplet techniques have been implemented in the fields of chemistry and biology for research in antibody handling, 6 high-throughput screening, 7 the synthesis of nanoparticles, 8,9 protein expressions, [10][11][12] polymerase chain reaction, [13][14][15][16][17] single-cell analysis, [18][19][20][21][22] and so forth.…”

Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures

“…Both mammalian cells as a single cell analysis [ 23 ] and bacteria as cell cultures [ 4 ] are currently studied in droplet microfluidics. Using microfluidic devices, simple monodisperse droplet generation with cells were developed [ 24 , 25 ] through single-cell analysis, as well as [ 19 , 26 ] selective encapsulation and [ 27 , 28 , 29 ] long-term incubation of cells [ 1 , 22 , 30 ].…”

Fabrication of PDMS microfluidic devices with 3D wax jetting

“…In the past 2 decades, microuidic chips have been widely applied for such purposes as micromixing and separating, 2,3 biological and chemical analysis, [4][5][6] and microemulsifying and encapsulating. 7,8 Based on the connectivity of their channels, microuidic chips can be roughly divided into 3 categories: 2 dimensional (planar), quasi 3D (stacked 2D planes) and real 3D networked micro channels. 9 The latter category consists of bridge features, where the channels can cross each other without connecting, and this enables these chips to perform complex processes like basket-weave owing.…”