Encapsulation of Vitamin C by Glycerol-Derived Dendrimers, Their Interaction with Biomimetic Models of Stratum corneum and Their Cytotoxicity

Abstract

Vitamin C is one of the most sensitive cosmetic active ingredients. To avoid its degradation, its encapsulation into biobased carriers such as dendrimers is one alternative of interest. In this work, we wanted to evaluate the potential of two biobased glycerodendrimer families (GlyceroDendrimers-Poly(AmidoAmine) (GD-PAMAMs) or GlyceroDendrimers-Poly(Propylene Imine) (GD-PPIs)) as a vitamin C carrier for topical application. The higher encapsulation capacity of GD-PAMAM-3 compared to commercial PAMAM-3 and different GD-PPIs, and its absence of cytotoxicity towards dermal cells, make it a good candidate. Investigation of its mechanism of action was done by using two kinds of biomimetic models of stratum corneum (SC), lipid monolayers and liposomes. GD-PAMAM-3 and VitC@GD-PAMAM-3 (GD-PAMAM-3 with encapsulated vitamin C) can both interact with the lipid representatives of the SC lipid matrix, whichever pH is considered. However, only pH 5.0 is suggested to be favorable to release vitamin C into the SC matrix. Their binding to SC-biomimetic liposomes revealed only a slight effect on membrane permeability in accordance with the absence of cytotoxicity but an increase in membrane rigidity, suggesting a reinforcement of the SC barrier property. Globally, our results suggest that the dendrimer GD-PAMAM-3 could be an efficient carrier for cosmetic applications.

Dendrimers: Synthesis, Encapsulation Applications and Specific Interaction with the Stratum Corneum—A Review

“…Recently, we further studied the ability of glycerodendrimers GD-PAMAMs and GD-PPIs to encapsulate vitamin C and its interaction with the stratum corneum. We showed that GD-PAMAM-3 is the best candidate for cosmetic application due to its good vitamin C encapsulation capacity, its low cytotoxicity and its interaction with the stratum corneum [14].…”

“…In recent years, we have developed various dendrimers containing biobased moieties to make these compounds more ecoor biocompatible. These have been used in catalysis, medical imaging, depollution and cosmetic fields [11][12][13][14][15][16].…”

Recent advances in multifunctional dendrimer‐based complexes for cancer treatment

“…Encapsulation of a drug into the dendrimer (cited by Bacha et al, 2023, Creative Commons Attribution [CC BY] license).…”

“…Above this generation, the size of the PAMAM dendrimer cannot increase due to the lack of free space for branch growth. In general, small generations of dendrimers have an open, mobile, and asymmetric structure, and with increasing generation, this structure becomes more globular and densely packed (Bacha et al, 2023; Fréchet, 2003; Majoral et al, 2002; Merino et al, 2001; Ortega et al, 2006; Tomalia & Khanna, 2016; Vlasov, 2006).…”

A Color-Detectable Vitamin C Controlled-Release System Fabricated Using Electrospinning

“…Encapsulation using polymeric materials is commonly employed for stabilizing unstable organic substances [4][5][6]. Well known techniques such as solvent evaporation, spray drying, and electrospinning have been widely used for this purpose.…”