Endocrine Control of Trophoblast-Uterine Epithelial Cell Interactions

Burghardt, Robert C.; Bowen, Jeffery A.; Bazer, Fuller W.

doi:10.1007/978-1-4612-1804-3_8

Cited by 2 publications

(2 citation statements)

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“…Since uterine endometrial epithelial cells do not undergo significant proliferation during early pregnancy (Ka et al, 2001), it is not expected that KGF affects endometrial epithelial cell proliferation, but it may affect uterine epithelial cell differentiation. During early pregnancy, uterine endometrial epithelial cells undergo differentiation to increase uterine receptivity to implanting blastocysts (Burghardt et al, 1998). The uterine endometrium synthesizes and secretes a variety of proteins to nourish the early conceptus during this period (Roberts and Bazer, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…The increased secretory activity in the porcine uterine epithelial cells during early pregnancy includes secretion of uteroferrin, retinol-binding protein and plasmin/trypsin inhibitor (Roberts and Bazer, 1988). The expression of cell surface molecules including Muc-1, integrin receptor subunits such as α4, α5 and β1, and extracellular matrix components, changes during the blastocyst adhesion and implantation process (Bowen et al, 1996;Burghardt et al, 1998). In pigs, expression of integrin receptor subunits α4, α5 and β1 on the endometrial epithelial cells increases during early pregnancy, while Muc-1 expression decreases.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Keratinocyte Growth Factor on the Uterine Endometrial Epithelial Cells in Pigs

Ka¹,

Bazer²

2005

Asian Australas. J. Anim. Sci

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Keratinocyte growth factor (KGF) functions in epithelial growth and differentiation in many tissues and organs. KGF is expressed in the uterine endometrial epithelial cells during the estrous cycle and pregnancy in pigs, and receptors for KGF (KGFR) are expressed by conceptus trophectoderm and endometrial epithelia. KGF has been shown to stimulate the proliferation and differentiation of conceptus trophectoderm. However, the role of KGF on the endometrial epithelial cells has not been determined. Therefore, this study determined the effect of KGF on proliferation and differentiation of endometrial epithelial cells in vitro and in vivo using an immortalized porcine luminal epithelial (pLE) cell line and KGF infusion into the uterine lumen of pigs between Days 9 and 12 of estrous cycle. Results showed that KGF did not stimulate proliferation of uterine endometrial epithelial cells in vitro and in vivo determined by the [ 3 H]thymidine incorporation assay and the proliferating cell nuclear antigen staining, respectively. Effects of KGF on expression of several markers for epithelial cell differentiation, including integrin receptor subunits α4, α5 and β1, plasmin/trypsin inhibitor, uteroferrin and retinol-binding protein were determined by RT-PCR, Northern and slot blot analyses, and immunohistochemisty, and KGF did not affect epithelial cell differentiation in vitro and in vivo. These results show that KGF does not induce epithelial cell proliferation and differentiation, suggesting that KGF produced by endometrial epithelial cells acts on conceptus trophectoderm in a paracrine manner rather than on endometrial epithelial cells in an autocrine manner.

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Section: Introductionmentioning

confidence: 99%