Endocytosis and degradation of chondroitin sulphate by liver endothelial cells

Smedsrød, Bård; Kjellén, Lena; Pertoft, Håkan

doi:10.1042/bj2290063

Cited by 68 publications

(29 citation statements)

References 16 publications

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“…In addition to HA, stabilin-2 has been shown to bind and clear proteoglycans such as chondroitin sulfate proteoglycan, and serglycin from the circulation (Smedsrød et al 1985;Øynebråten et al 2000). HA and proteoglycans are highly expressed in ocular tissues, where they, for example, contribute to the stromal transparency of the cornea (Tanihara et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Expression of stabilin-2, a novel fasciclin-like hyaluronan receptor protein, in murine sinusoidal endothelia, avascular tissues, and at solid/liquid interfaces

Falkowski

Schledzewski

Hansen

et al. 2003

Histochemistry and Cell Biology

127

143

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Stabilin-2, the hepatic hyaluronan receptor, has recently been cloned by us. Together with stabilin-1, stabilin-2 constitutes a novel family of fasciclin-like hyaluronan receptor homologues. Here, we analyzed expression of stabilin-2 (mStab-2) in a broad array of C57BL/6 mouse organs and tissues. While northern blot analysis showed positive expression of mStab-2 mRNA confined to liver and spleen, immunohistochemistry demonstrated mStab-2 protein expression in the endothelial sinuses of liver, lymph nodes, spleen, and bone marrow, and in specialized structures of eye, heart, brain, and kidney. Expression of mStab-2 was detected in corneal and lens epithelium, in mesenchymal cells of the heart valves, in the ependymal cells lining the ventricles in the brain, and in the prismatic epithelial cells covering the renal papillae. In pathological conditions, such as tumor growth or wound healing processes, mStab-2 was not expressed in the newly formed vasculature or other tissue components. Based on these results, we suggest that mStab-2 might be involved in the clearance of hyaluronan from the lymph or the blood circulation via the network of endothelial sinuses. At the other mStab-2-positive tissues sites that are either avascular and/or demarcate a solid/liquid interface, mStab-2 may serve to maintain tissue integrity by supporting extracellular matrix turnover or it may contribute to maintaining fluidity of bodily liquids by resorption of hyaluronan.

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Section: Discussionmentioning

confidence: 99%

Expression of stabilin-2, a novel fasciclin-like hyaluronan receptor protein, in murine sinusoidal endothelia, avascular tissues, and at solid/liquid interfaces

Falkowski

Schledzewski

Hansen

et al. 2003

Histochemistry and Cell Biology

127

143

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“…Then, Blomhoff et al 8 showed that formaldehyde-denatured albumin was preferentially taken up by SEC in vivo and in vitro. More recently, Smedsrød et al 9 revealed that advanced glycation end product (AGE)-modified bovine serum albumin (BSA), which is the nonenzymically glycated BSA, were efficiently incorporated into cultured SEC by endocytosis. The receptors for these modified or denatured macromolecules are known as "scavenger receptors," which are originally found on the surface of the macrophage.…”

Section: Functions Of Secmentioning

confidence: 99%

Cell biology and pathology of liver sinusoidal endothelial cells

Enomoto

Nishikawa

Omori

et al. 2004

Med Electron Microsc

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Growing evidence revealed that liver sinusoidal endothelial cells (SEC) play several important roles in physiology and pathology of the liver. It has been well understood that their structural characteristics, such as the membrane sieve and lack of basement membrane, facilitate direct contact of soluble and insoluble serum substances with hepatic parenchymal cells, resulting in enhancement of hepatic metabolic activity. In addition, SEC is now regarded as a member of the scavenger endothelial cells, which have potential to eliminate a variety of macromolecules from the blood circulation by receptor-mediated endocytosis. It is reported that molecules preferentially eliminated by SEC are denatured or modified proteins such as advanced glycation end products, extracellular matrix components including hyaluronic acid, and some lipoproteins. The nature of the scavenger receptors corresponding to these molecules remains to be clarified. Recently, it was noted that SEC has an antigen-presenting function similar to dendritic cells. Taken together, it is suggested that SEC, cooperating with Kupffer cells and hepatic dendritic cells, may partake of immunoregulatory functions in the liver. SEC also plays a pivotal role in the pathological process of ischemia-reperfusion injury following liver surgery and liver transplantation. Thus, it is of importance to elucidate the mechanisms of apoptosis and proliferation of SEC. Recent results on the regulation of growth and apoptotic signaling of SEC are discussed.

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“…In conclusion, our results indicate: 1) that the majority of the HABP of SV-3T3 cells is exposed at the outer surface of the plasma membrane; 2) that the HABP has the properties of an integral membrane protein in that detergents are required for its extraction [8] and in that it can be reconstituted back into lipid vesicle membranes; 3) that the high affinity of binding of HA to the plasma membrane is in part dependent "1 [19] from the circulation. However the precise relationship of the HABP from SV3T3 cells to these HA-binding sites and to other HABPs recently described in other laboratories is not yet known.…”

Section: Properties Of Habp Reconstituted Into Lipid Vesiclesmentioning

confidence: 97%

Hyaluronate‐binding protein of simian virus 40‐transformed 3T3 cells: Membrane distribution and reconstitution into lipid vesicles

Chi-Rosso

Toole

1987

J of Cellular Biochemistry

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Hyaluronate-binding protein (HABP) has been extracted in detergent from the membranes of simian virus 40-transformed 3T3 (SV-3T3) cells (Underhill et al, J Biol Chem 258:8086-8091, 1983). When SV-3T3 cells were treated with trypsin prior to isolation and dissolution of the membranes, no hyaluronate-binding activity could be detected. This indicates that all of the detectable HABP of SV-3T3 cells is located on the external surface of the plasma membrane rather than on internal membranes, which would be inaccessible to the trypsin. The detergent-extracted HABP from SV-3T3 membranes was reconstituted into the membrane of lipid vesicles, which were formed by addition of exogenous phosphatidylcholine and cholic acid to the extracts followed by removal of detergent by dialysis against 0.02 M Tris pH 8.0 in the presence of protease inhibitors. Reconstitution was assessed by sedimentation in a discontinuous sucrose gradient and by gel filtration on Sepharose 4B in the presence and absence of detergent. The characteristics of binding of hyaluronate to the reconstituted HABP were then compared with those studied previously for the original membrane-bound HABP and the detergent-extracted HABP (Underhill et al, J Biol Chem 258:8086-8091, 1983). It was observed previously that binding of hyaluronate to HABP in the cell membranes was of higher affinity and specificity than to HABP in the detergent extracts of these membranes. It was found here that reconstitution of the extracted HABP into the membranes of lipid vesicles led to restoration of affinity of binding to the level observed in the original cell membranes. However, whereas chondroitin sulfate does not compete significantly for binding of hyaluronate to cell membrane-bound HABP, partial competition was observed for the reconstituted HABP as well as for detergent-extracted HABP. Thus, it is concluded that the high affinity of binding of hyaluronate to the plasma membrane of SV-3T3 cells is in part dependent on insertion of the HABP in the membrane, but that other interactions, not duplicated in our reconstitution experiments, must be necessary for the specificity of the HABP.

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Endocytosis and degradation of chondroitin sulphate by liver endothelial cells

Cited by 68 publications

References 16 publications

Expression of stabilin-2, a novel fasciclin-like hyaluronan receptor protein, in murine sinusoidal endothelia, avascular tissues, and at solid/liquid interfaces

Expression of stabilin-2, a novel fasciclin-like hyaluronan receptor protein, in murine sinusoidal endothelia, avascular tissues, and at solid/liquid interfaces

Cell biology and pathology of liver sinusoidal endothelial cells

Hyaluronate‐binding protein of simian virus 40‐transformed 3T3 cells: Membrane distribution and reconstitution into lipid vesicles

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