Endophytic fungi isolated from Brazilian medicinal plants as potential producers of antioxidants and their relations with anti-inflammatory activity

Santos, Tamara Fonseca Bastos; Carvalho, Cleoni dos Santos; Almeida, Mônica Aparecida de; Delforno, Tiago Palladino; Duarte, Iolanda Cristina Silveira

doi:10.1007/s13205-020-02211-7

Cited by 19 publications

(7 citation statements)

References 35 publications

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“…The bonds in the region of 802–932 cm-1 can be related to the presence of aromatic C–H groups 14 . The appeared peaks at around 1260 and 1408 cm -1 were associated with—C–OH and C=O, respectively 15 and these peaks are similar to those found for the FTIR spectra of flavonoid quercetin-5′-sulfonated acid 16 . The broad bonds at around 3366, 3382, 3260, and 3379 cm -1 were attributed to the stretching vibration of –OH groups which can be associated with alcohols and phenols 17 .…”

Section: Resultssupporting

confidence: 71%

The anticancer and antibacterial potential of bioactive secondary metabolites derived From bacterial endophytes in association with Artemisia absinthium

Damavandi,

Shojaei,

Esfahani

2023

Sci Rep

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The continuous search for secondary metabolites in microorganisms isolated from untapped reservoirs is an effective prospective approach to drug discovery. In this study, an in-depth analysis was conducted to investigate the diversity of culturable bacterial endophytes present in the medicinal plant A. absinthium, as well as the antibacterial and anticancer potential of their bioactive secondary metabolites. The endophytic bacteria recovered from A. absinthium, were characterized via the implementation of suitable biochemical and molecular analyses. Agar well diffusion and broth microdilution were used to screen antibacterial activity. SEM was performed to assess the impact of the extracted metabolite on MRSA strain cell morphology. Apoptosis and cytotoxicity assays were used to evaluate anticancer activity against MCF7 and A549. The FTIR, GC–MS were used to detect bioactive compounds in the active solvent fraction. Of the various endophytic bacteria studied, P. aeruginosa SD01 showed discernible activity against both bacterial pathogens and malignancies. The crude ethyl acetate extract of P. aeruginosa SD01 showed MICs of 32 and 128 µg/mL for S. aureus and MRSA, respectively. SEM examination demonstrated MRSA bacterial cell lysis, hole development, and intracellular leaking. This study revealed that the crude bioactive secondary metabolite SD01 has potent anticancer activity. In this study, 2-aminoacetophenone, 1,2-apyrazine-1,4-dione, phenazine and 2-phenyl-4-cyanopyridine were the major bioactive secondary metabolites. In conclusion, our findings indicate that the bacteria recovered from A. absinthium plants and in particular, P. aeruginosa SD01 is a remarkable source of untapped therapeutic, i.e., antimicrobial and anticancer compounds.

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Section: Resultssupporting

confidence: 71%

The anticancer and antibacterial potential of bioactive secondary metabolites derived From bacterial endophytes in association with Artemisia absinthium

Damavandi,

Shojaei,

Esfahani

2023

Sci Rep

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“…Furthermore, some endophytic fungi established interaction with their host plants and co-evolved with them. They can produce valuable secondary metabolites like as their hosts, regarded as a novel and important source of bioactive compounds with potential biotechnological applications in the context of agriculture, food, cosmetics, and medicine [13][14][15]. In recent years, numerous studies have reported that endophytic fungi producing Huperzine A have been isolated from from different Huperiaceae, which offers an alternative method to reduce the immense plant harvest-related logistics needed to meet the increasing demand for the Hup A [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Diversity of endophytic fungal community in Huperzia serrata from different ecological areas and their correlation with Hup A content

et al. 2022

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Background Huperzine A (Hup A) has attracted considerable attention as an effective therapeutic candidate drug used to treat Alzheimer’s disease. Whereas, the production of Hup A from wild plants faced a major challenge, which is the wild Huperzia Serrata harbor a low Hup A content, has a long-life cycle, and has a small yield. At present, several reports showed that Hup A is produced by various endophytic fungal strains isolated from H. serrata, thereby providing an alternative method to produce the compound and reduce the consumption of this rare and endangered plant. However, till now, very few comprehensive studies are available on the biological diversity and structural composition of endophytic fungi and the effects of endophytic fungi on the Hup A accumulation in H. serrata. Results In this research, the composition and diversity of fungal communities in H. serrata were deciphered based on high-throughput sequencing technology of fungal internal transcribed spacer regions2 (ITS2). The correlation between endophytic fungal community and Hup A content was also investigated. Results revealed that the richness and the diversity of endophytic fungi in H. serrata was various according to different tissues and different ecological areas. The endophytic fungal communities of H. serrata exhibit species-specific, ecological-specific, and tissue-specific characteristics. There are 6 genera (Ascomycota_unclassified, Cyphellophora, Fungi_unclassified, Sporobolomyces, and Trichomeriaceae_unclassified) were significantly positively correlated with Hup A content in all two areas, whereas, there are 6 genera (Auricularia, Cladophialophora, Cryptococcus, Mortierella, and Mycena) were significantly negatively correlated with Hup A content of in all two areas. Conclusions This study indicated a different composition and diverse endophytic fungal communities in H. serrata from different organs and ecological areas. The current study will provide the realistic basis and theoretical significance for understanding the biological diversity and structural composition of endophytic fungal communities in H. serrata, as well as providing novel insights into the interaction between endophytic fungi and Hup A content.

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“…In our fungal isolates, we found pathogens of plant diseases from genera such as Pestalotiopsis [ 58 , 59 ], Volutella [ 60 , 61 ], Didymella [ 62 ], Xylaria [ 63 ], and Crinipellis [ 64 ]; the genus Coriolopsis which is associated with trees and one of the lignicolous fungi [ 65 ]; and Paraphaeosphaeria [ 66 ], Peroneutypa [ 67 ], and Phaeophlebiopsis [ 68 ], which are genera associated with the epiphytic fungi of plants. The fungi of the genus Absidia are usually isolated from soil, constituting the pathogens of many human diseases [ 69 , 70 , 71 , 72 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity of Fungi Isolated from Potato Nematode Cysts in Guizhou Province, China

Zhang

Yang

Jiang

et al. 2023

JoF

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Potatoes rank third in terms of human consumption after rice and wheat. Globodera spp. are significant pests of potato crop worldwide. Globodera rostochiensis was found in Weining County, Guizhou Province, China, in 2019. We collected soil from the rhizosphere zone from infected potato plants and separated mature cysts through simple floatation and sieving methods. The selected cysts were surface-sterilized, and the colonized fungi were isolated and purified. At the same time, the preliminary identification of fungi and fungi parasites on the cysts of nematodes was carried out. This study aimed to define the species and frequency of fungi-colonizing cysts of G. rostochiensis collected from Weining County, Guizhou Province, China, and provide a basis for the control of G. rostochiensis. As a result, 139 strains of colonized fungi were successfully isolated. Multigene analyses showed that these isolates included 11 orders, 17 families, and 23 genera. The genera Fusarium (with a separation frequency of 59%), Penicillium (11%), Edenia (3.6%), and Paraphaeosphaeria (3.6%) were the most frequently occurring. Among the 44 strains, 27 had a colonization rate of 100% on the cysts of G. rostochiensis. Meanwhile, the functional annotation of 23 genera indicated that some fungi have multitrophic lifestyles combining endophytic, pathogenic, and saprophytic behavior. In conclusion, this study showed the species composition and lifestyle diversity of colonized fungi from G. rostochiensis and demonstrated these isolates as potential sources of biocontrol agents. Colonized fungi were isolated from G. rostochiensis for the first time in China, and the taxonomic diversity of fungi from G. rostochiensis was clarified.

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Endophytic fungi isolated from Brazilian medicinal plants as potential producers of antioxidants and their relations with anti-inflammatory activity

Cited by 19 publications

References 35 publications

The anticancer and antibacterial potential of bioactive secondary metabolites derived From bacterial endophytes in association with Artemisia absinthium

The anticancer and antibacterial potential of bioactive secondary metabolites derived From bacterial endophytes in association with Artemisia absinthium

Diversity of endophytic fungal community in Huperzia serrata from different ecological areas and their correlation with Hup A content

Diversity of Fungi Isolated from Potato Nematode Cysts in Guizhou Province, China

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