Endophytic population of Pantoea agglomerans in citrus plants and development of a cloning vector for endophytes

Andreote, Fernando Dini; Rossetto, P. B.; Souza, Larissa Gonçalves; Marcon, Joelma; Maccheroni, Walter; Azevedo, João Lúcio; Araújo, Welington Luiz

doi:10.1002/jobm.200700341

Cited by 15 publications

(7 citation statements)

References 26 publications

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“…These results suggest that this strain could be used as an inoculant to improve sugarcane productivity. Andreote et al [139], Ferreira et al [140] and De Lima Procopio et al [141], have proposed using the endophytic, plasmid-harbouring P. agglomerans strains abundantly colonizing seedlings of citrus plants and eucalyptuses, as vehicles for the introduction of exogenous genes promoting growth of these cultivars.…”

Section: Growth Promotion Of Cultivable Plantsmentioning

confidence: 99%

Pantoea agglomerans: a mysterious bacterium of evil and good. Part IV. Beneficial effects

Dutkiewicz¹,

Maćkiewicz²,

Lemieszek

et al. 2016

Ann Agric Environ Med.

135

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Pantoea agglomerans, a gammaproteobacterium of plant origin, possesses many beneficial traits that could be used for the prevention and/or treatment of human and animal diseases, combating plant pathogens, promotion of plant growth and bioremediation of the environment. It produces a number of antibiotics (herbicolin, pantocins, microcin, agglomerins, andrimid, phenazine, among others) which could be used for combating plant, animal and human pathogens or for food preservation. Japanese researchers have demonstrated that the low-molecular-mass lipopolysaccharide of P. agglomerans isolated by them and described as 'Immunopotentiator from Pantoea agglomerans 1 (IP-PA1)' reveals the extremely wide spectrum of healing properties, mainly due to its ability for the maintenance of homeostasis by macrophage activation. IP-PA1 was proved to be effective in the prevention and treatment of a broad range of human and animal disorders, such as tumours, hyperlipidaemia, diabetes, ulcer, various infectious diseases, atopic allergy and stress-induced immunosuppression; it also showed a strong analgesic effect. It is important that most of these effects could be achieved by the safe oral administration of IP-PA1. Taking into account that P. agglomerans occurs commonly as a symbiont of many species of insects, including mosquitoes transmitting the Plasmodium parasites causing malaria, successful attempts were made to apply the strategy of paratransgenesis, in which bacterial symbionts are genetically engineered to express and secrete anti-Plasmodium effector proteins. This strategy shows prospects for a successful eradication of malaria, a deadly disease killing annually over one million people, as well as of other vector-borne diseases of humans, animals and plants. Pantoea agglomerans has been identified as an antagonist of many plant pathogens belonging to bacteria and fungi, as a result of antibiotic production, competition mechanisms or induction of plant resistance. Its use as a biocontrol agent permits the decrease of pesticide doses, being a healthy and environmental-friendly procedure. The application of the preparations of this bacterium efficiently protects the stored pome, stone and citrus fruits against invasion of moulds. P. agglomerans strains associated with both rhizosphere and plant tissues (as endophytes) efficiently promote the growth of many plants, including rice and wheat, which are the staple food for the majority of mankind. The promotion mechanisms are diverse and include fixation of atmospheric nitrogen, production of phytohormones, as well as degradation of phytate and phosphate solubilizing which makes the soil phosphorus available for plants. Accordingly, P. agglomerans is regarded as an ideal candidate for an environmental-friendly bioinoculant replacing chemical fertilizers. It has been documented that the Pantoea strains show biodegradation activity on various chemical pollutants of soil and water, including petroleum hydrocarbons and toxic metals. P. agglomerans prevents the penetration of harmful...

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Section: Growth Promotion Of Cultivable Plantsmentioning

confidence: 99%

Pantoea agglomerans: a mysterious bacterium of evil and good. Part IV. Beneficial effects

Dutkiewicz¹,

Maćkiewicz²,

Lemieszek

et al. 2016

Ann Agric Environ Med.

135

View full text Add to dashboard Cite

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“…Some plant endophytes, which are organisms that live inside of plants often asymptomatically, have been shown to exhibit human pathogenic potential. Species like Morganella morganii , Klebsiella pneumoniae , Pantoea agglomerans and Streptomyces sp., have clinical relevance, but have been more closely investigated for their association with plants [93,94]. M. morganii is a phosphate solubilising bacterium [95], K. pneumoniae a nitrogen-fixing endophyte [96], P. agglomerans a gall-forming phytopathogen, and Streptomyces sp.…”

Section: Evolutionary Modelsmentioning

confidence: 99%

Insights into Cross-Kingdom Plant Pathogenic Bacteria

Kirzinger

Nadarasah

Stavrinides

2011

Genes

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Plant and human pathogens have evolved disease factors to successfully exploit their respective hosts. Phytopathogens utilize specific determinants that help to breach reinforced cell walls and manipulate plant physiology to facilitate the disease process, while human pathogens use determinants for exploiting mammalian physiology and overcoming highly developed adaptive immune responses. Emerging research, however, has highlighted the ability of seemingly dedicated human pathogens to cause plant disease, and specialized plant pathogens to cause human disease. Such microbes represent interesting systems for studying the evolution of cross-kingdom pathogenicity, and the benefits and tradeoffs of exploiting multiple hosts with drastically different morphologies and physiologies. This review will explore cross-kingdom pathogenicity, where plants and humans are common hosts. We illustrate that while cross-kingdom pathogenicity appears to be maintained, the directionality of host association (plant to human, or human to plant) is difficult to determine. Cross-kingdom human pathogens, and their potential plant reservoirs, have important implications for the emergence of infectious diseases.

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“…Some, but not all, P. agglomerans strains can stably maintain E. coli pBR322 plasmid-based vectors and can be electroporated with derivatives of pBR322 (Wright et al, 2001 ). Some others can be electroporated with shuttle vectors constructed by cloning in the E. coli pUC18 plasmid of small cryptic plasmids identified in certain endophytic P. agglomerans strains (Andreote et al, 2008 ; de Lima Procópio et al, 2011 ). However, the use of the tools mentioned above is limited due to the limit of cloned insert size and the failure of their introduction to certain P. agglomerans strains.…”

Section: Discussionmentioning

confidence: 99%

Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

Giermasińska-Buczek,

Gawor,

Stefańczyk

et al. 2024

Front. Microbiol.

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P1 is a model, temperate bacteriophage of the 94 kb genome. It can lysogenize representatives of the Enterobacterales order. In lysogens, it is maintained as a plasmid. We tested P1 interactions with the biocontrol P. agglomerans L15 strain to explore the utility of P1 in P. agglomerans genome engineering. A P1 derivative carrying the Tn9 (cmR) transposon could transfer a plasmid from Escherichia coli to the L15 cells. The L15 cells infected with this derivative formed chloramphenicol-resistant colonies. They could grow in a liquid medium with chloramphenicol after adaptation and did not contain prophage P1 but the chromosomally inserted cmR marker of P1 Tn9 (cat). The insertions were accompanied by various rearrangements upstream of the Tn9 cat gene promoter and the loss of IS1 (IS1L) from the corresponding region. Sequence analysis of the L15 strain genome revealed a chromosome and three plasmids of 0.58, 0.18, and 0.07 Mb. The largest and the smallest plasmid appeared to encode partition and replication incompatibility determinants similar to those of prophage P1, respectively. In the L15 derivatives cured of the largest plasmid, P1 with Tn9 could not replace the smallest plasmid even if selected. However, it could replace the smallest and the largest plasmid of L15 if its Tn9 IS1L sequence driving the Tn9 mobility was inactivated or if it was enriched with an immobile kanamycin resistance marker. Moreover, it could develop lytically in the L15 derivatives cured of both these plasmids. Clearly, under conditions of selection for P1, the mobility of the P1 selective marker determines whether or not the incoming P1 can outcompete the incompatible L15 resident plasmids. Our results demonstrate that P. agglomerans can serve as a host for bacteriophage P1 and can be engineered with the help of this phage. They also provide an example of how antibiotics can modify the outcome of horizontal gene transfer in natural environments. Numerous plasmids of Pantoea strains appear to contain determinants of replication or partition incompatibility with P1. Therefore, P1 with an immobile selective marker may be a tool of choice in curing these strains from the respective plasmids to facilitate their functional analysis.

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Endophytic population of Pantoea agglomerans in citrus plants and development of a cloning vector for endophytes

Cited by 15 publications

References 26 publications

<i>Pantoea agglomerans</i>: a mysterious bacterium of evil and good. Part IV. Beneficial effects

<i>Pantoea agglomerans</i>: a mysterious bacterium of evil and good. Part IV. Beneficial effects

Insights into Cross-Kingdom Plant Pathogenic Bacteria

Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

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