Endosulfan in vitro toxicity in Atlantic salmon hepatocytes obtained from fish fed either fish oil or vegetable oil

Krøvel, Anne Vatland; Søfteland, Liv; Torstensen, Bente E.

doi:10.1016/j.cbpc.2009.10.003

Cited by 20 publications

(23 citation statements)

References 52 publications

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“…Both of these studies observed changes in the appearance of liver tissue including large spherical vesicles in hepatocytes and characteristics of lipid deposition. The appearance of these structures also in in the hepatic tissue of A. bimaculatus suggests that the insecticide may have affected lipid deposition, as was also observed by Braunbeck et al () and Krovel et al ().…”

Section: Discussionsupporting

confidence: 69%

Histological and Histometric Evaluation of the Liver in Astyanax Bimaculatus (Teleostei: Characidae), Exposed to Different Concentrations of an Organochlorine Insecticide

Marcon

Bazzoli

Mounteer

et al. 2015

The Anatomical Record

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To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan V R for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 lg L 21 of Thiodan V R and a control group without insecticide. The action of Thiodan V R at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan V R in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan V R also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan V R analyzed, the morphology of hepatic tissue had a cellular adaptive response.

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Section: Discussionsupporting

confidence: 69%

Histological and Histometric Evaluation of the Liver in Astyanax Bimaculatus (Teleostei: Characidae), Exposed to Different Concentrations of an Organochlorine Insecticide

Marcon

Bazzoli

Mounteer

et al. 2015

The Anatomical Record

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“…Recent studies have shown a strong correlation between chemical exposure and steatosis (fatty liver) [43]. We have earlier shown that the organochlorine insecticide endosulfan induces steatosis in Atlantic salmon hepatocytes [44], while Sun et al [45] showed a similar response in male rockfish ( Sebastiscus marmoratus ) exposed to the triazole-containing fungicide paclobutrazol. As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon

Berntssen¹,

Søfteland²

2015

PLoS ONE

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The aim of this study was to elucidate how vitamin E (alpha tocopherol) may ameliorate the toxicity of the pesticide chlorpyrifos in Atlantic salmon. Freshly isolated hepatocytes were exposed to vitamin E, chlorpyrifos or a combination of vitamin E and chlorpyrifos (all 100 μM). Transcriptomics (RNA-seq) and metabolomics were used to screen for effects of vitamin E and chlorpyrifos. By introducing vitamin E, the number of upregulated transcripts induced by chlorpyrifos exposure was reduced from 941 to 626, while the number of downregulated transcripts was reduced from 901 to 742 compared to the control. Adding only vitamin E had no effect on the transcriptome. Jak-STAT signaling was the most significantly affected pathway by chlorpyrifos treatment according to the transcriptomics data. The metabolomics data showed that accumulation of multiple long chain fatty acids and dipeptides and amino acids in chlorpyrifos treated cells was partially alleviated by vitamin E treatment. Significant interaction effects between chlorpyrifos and vitamin E were seen for 15 metabolites, including 12 dipeptides. The antioxidant had relatively modest effects on chlorpyrifos-induced oxidative stress. By combining the two data sets, the study suggests that vitamin E supplementation prevents uptake and accumulation of fatty acids, and counteracts inhibited carbohydrate metabolism. Overall, this study shows that vitamin E only to a moderate degree modifies chlorpyrifos toxicity in Atlantic salmon liver cells.

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“…From these cell cultures cell proliferative activity was suppressed most intensively in PK15 culture at the concentrations of 10 -1 -10 -6 M of endosulfan. Krovel et al 21 observed moderate cytotoxicity and steatosis in a dose-dependent manner in the primary cultures of hepatocytes isolated from Atlantic salmon exposed to concentrations of 0.001-100 microM endosulfan for 48 h. Skandrani et al 22 investigated endosulfan toxicity in neuronal SH-SY5Y and pulmonary A549 cell lines exposed to increasing pesticide concentrations for 3 days. This experiment showed that neuronal cells were more sensitive than pulmonary cells.…”

Section: Discussionmentioning

confidence: 99%

İnsektisit Endosülfana Maruz Kalan Hayvansal ve Böcek Kökenli Hücre Kültürlerindeki Proliferasyon ve LDH Fazlalığı

Kovalkovičová¹,

Pistl²,

Csank³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryIn the present study three different cell cultures, derived from rabbit kidney (RK13), rat liver (WBF344) and insect origin (Sf21), were used to examine the cytotoxic effect of the insecticide endosulfan. Cytotoxicity was determined on the basis of cell proliferation activity; the cellular damage was assessed by evaluation of cytopathic effect and lactate dehydrogenase (LDH) leakage. Endosulfan treatment suppressed proliferative activity in cell cultures as follows: insect Sf21 cells (10 -2 M (P<0.05) miktarında belirlendi. Bu sonuçlar endosülfan maruziyetine karşı hücre tipine bağlı duyarlılığı işaret etmektedir. Endosülfan, memeli hücre kültürüne göre böcek kökenli hücre kültürlerindeki hücre sayılarında daha belirgin bir düşüşe neden olsa da LDH fazlalığı ve hücredeki mikroskobik hasar belirteçleri en belirgin olarak karaciğer hücrelerinde görülmektedir.

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Endosulfan in vitro toxicity in Atlantic salmon hepatocytes obtained from fish fed either fish oil or vegetable oil

Cited by 20 publications

References 52 publications

Histological and Histometric Evaluation of the Liver in Astyanax Bimaculatus (Teleostei: Characidae), Exposed to Different Concentrations of an Organochlorine Insecticide

Histological and Histometric Evaluation of the Liver in Astyanax Bimaculatus (Teleostei: Characidae), Exposed to Different Concentrations of an Organochlorine Insecticide

Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon

İnsektisit Endosülfana Maruz Kalan Hayvansal ve Böcek Kökenli Hücre Kültürlerindeki Proliferasyon ve LDH Fazlalığı

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