Enemy at the gates: forward genetics of the mouse antiviral response

Kielczewska, Agnieszka; Vidal, Silvia M.

doi:10.1016/j.coi.2006.07.009

Cited by 4 publications

(3 citation statements)

References 77 publications

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“…1 NK-cell recognition of infected or transformed cells depends on the expression of stress-induced selfligands or pathogen-encoded ligands that are detected by activating receptors. 2 Similarly, cells that are rapidly proliferating or have experienced DNA damage often express stress-induced ligands that trigger activating receptors on NK cells. 3 One shared trait of viruses and tumors is to avoid detection by CD8 ϩ T cells by down-regulating the expression of major histocompatibility complex (MHC) class I.…”

Section: Introductionmentioning

confidence: 99%

The requirement for NKG2D in NK cell–mediated rejection of parental bone marrow grafts is determined by MHC class I expressed by the graft recipient

Beilke

Benjamin

Lanier

2010

Blood

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Section: Introductionmentioning

confidence: 99%

The requirement for NKG2D in NK cell–mediated rejection of parental bone marrow grafts is determined by MHC class I expressed by the graft recipient

Beilke

Benjamin

Lanier

2010

Blood

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“…However, as phenotype scoring is the access gate to the study of the underlying genetic mechanism (Hotz et al, 2006), a comprehensive and thorough screening process becomes the key to their use. Analyzing or even finding phenotypes related to the process or tissue of interest is especially difficult when these are not easily accessible to assay (Kielczewska and Vidal, 2006). A wide variety of approaches has been used, from classical bare-eye observation (Kuromori et al, 2006) to complex functional in vivo and in vitro assays (Burrack and Higgins, 2007).…”

Section: Discussionmentioning

confidence: 99%

A PCR-based forward genetics screening, using expression domain-specific markers, identifies mutants in endosperm transfer cell development

et al. 2014

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Mutant collections are an invaluable source of material on which forward genetic approaches allow the identification of genes affecting a wide variety of biological processes. However, some particular developmental stages and morphological structures may resist analysis due to their physical inaccessibility or to deleterious effects associated to their modification. Furthermore, lethal mutations acting early in development may escape detection. We have approached the characterization of 101 maize seed mutants, selected from a collection of 27,500 visually screened Mu-insertion lines, using a molecular marker approach based on a set of genes previously ascribed to different tissue compartments within the early developing kernel. A streamlined combination of qRT-PCR assays has allowed us to preliminary pinpoint the affected compartment, establish developmental comparisons to WT siblings and select mutant lines with alterations in the different compartments. Furthermore, clusters of markers co-affected by the underlying mutation were identified. We have analyzed more extensively a set of lines presenting significant variation in transfer cell-associated expression markers, and have performed morphological observations, and immunolocalization experiments to confirm the results, validating this approach as an efficient mutant description tool.

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“…Target identification with forward genetic screening is more laborious than with reverse genetics, but in principle straightforward. Forward genetic screening has already been covered in excellent reviews [10][11][12][13] and will not be further discussed. With the arrival of the gene silencing techniques employing shRNA (short hairpin RNA) or siRNA (small interfering RNA), expression of specific gene products can be downregulated.…”

Section: Reverse Genetic Screeningmentioning

confidence: 99%