Reciprocal chemical genetics for swift lead and target identification

Kuijl, Coenraad; Tuin, Adriaan W.; Overkleeft, Hermen S.; Neefjes, Jacques

doi:10.1039/b803265n

Cited by 2 publications

(1 citation statement)

References 47 publications

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“…Because of its considerable mass in the body, skeletal muscle may constitute a convenient and abundant source of stem cells, and thus has received considerable recent attention for its potential in cellular and cell‐mediated therapies (Passier et al , 2003). Muscle‐derived stem cells (MDSCs) can be isolated from skeletal muscles (Seale et al , 2001), and have been shown to differentiate toward several different lineages in vitro and in vivo in response to genetic manipulation, changes in the extracellular environment or stimulation with intrinsic regulators or factors, including growth factors and other signalling molecules (Stocum, 2004; Jackson et al , 2009; Kuijl et al , 2008). Thus, the recent interest in using MDSCs as a source material for cell therapy is perhaps not surprising.…”

Section: Introductionmentioning

confidence: 99%

Potential induction of rat muscle-derived stem cells to neural-like cells by retinoic acid

Kim

Kang

et al. 2010

J Tissue Eng Regen Med

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Several recent studies have demonstrated that stem cell differentiation can be generated by derivatives of retinoic acid. In this study we chose retinoic acid (RA) for inducing neural differentiation of rat muscle-derived stem cells (rMDSCs). rMDSCs were pre-induced with 10 ng/ml basic fibroblast growth factor (bFGF) and then treated with 2 μM RA. After stimulation, RA induced rMDSCs to have a neural-like morphology after 1-7 days of in vitro differentiation. In the results of immunocytochemistry, rMDSC treated with RA showed abundant positive cells against the neuronal markers neuronal-specific enolase (NSE) and tubulin-βIII (Tuj1). Also, 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)-positive cells were observed, indicating oligodendrocyte lineage cells. However, positive cells against glial fibrillary acidic protein (GFAP), marker of astrocytes, were not detected. The mRNA profile of these cells included higher expression of NSE compared with those of non-treated cells in real-time PCR. From the data in this work, we suggest that rMDSCs can trans-differentiate into a neural-like phenotype under the RA conditions.

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Section: Introductionmentioning

confidence: 99%