“…13 The degradation of organic substrate longitudinally through successive ABR compartments, coupled with spatial separation of sludge blankets in an ABR, can create functional differentiation of the mixed anaerobic consortia in each successive bioreactor compartment. 14,15 The ABR has been used for several applications, to include the degradation of food production wastewaters, industrial wastewaters, and domestic wastewater streams; 13,16,17 however, few studies of pilot-scale ABRs treating low temperature (6 to 24 °C) raw domestic wastewater over long timescales exist. 10,11,18,19 Of studies examining ABRs treating large volumes of raw domestic wastewater at low temperatures, most analysis centers on ABR operational performance with less attention paid to the development of the anaerobic microbial communities over time and space within the ABR sludge blanket communities, especially immediately after seeding.…”