Energy Transfer in Mixtures of Water- Soluble Oligomers: Effect of Charge, Aggregation, and Surfactant Complexation

Stork, Martin; Gaylord, Brent S.; Heeger, Alan J.; Bazan, Guillermo C.

doi:10.1002/1521-4095(20020304)14:5<361::aid-adma361>3.0.co;2-9

Cited by 197 publications

(213 citation statements)

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“…In all cases, a minimum is observed in the absorbance (corresponding to around 40% of the initial values), with the concentration at which this occurs depending upon the surfactant chain length (from 5 × 10 -5 M for dodecyl to 4 × 10 -2 M for butyl ( Figure 4A). A similar broadening of the absorption spectra and reduction in absorbance has previously been reported with the structurally related poly(9,9-bis(6′-(N,N,N-trimethylammonium)-hexyl)fluorenephenylene) copolymer and related oligomers upon addition of SDS, 22 although no explanation was presented.…”

Section: Resultssupporting

confidence: 71%

Modulating the Emission Intensity of Poly-(9,9-bis(6‘-N,N,N-trimethylammonium)hexyl)-Fluorene Phenylene) Bromide Through Interaction with Sodium Alkylsulfonate Surfactants

et al. 2007

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The interaction between the cationic HTMA-PFP (Poly-(9,9-bis(6′-N,N,N-trimethylammonium)hexyl-fluorene phenylene) bromide) and oppositely charged sodium n-alkyl sulfonate surfactants of different chain lengths has been studied in DMSO-water solutions (4% v/v) by UV-visible absorption, fluorescence spectroscopy, fluorescence lifetimes, electrical conductivity, and 1 H NMR spectroscopy. Polymer-surfactant interactions lead to complex spectroscopic behaviors which depends on surfactant concentration. At low surfactant concentrations, the observed strong static fluorescence quenching of fluorescence seems to be associated with formation of aggregates between polymer chains neutralized through interaction with surfactants. This is supported by conductivity and by analysis of absorption spectra deconvoluted at each surfactant concentration using an adapted iterative method. In contrast, above the surfactant critical micelle concentration, there is a strong fluorescence enhancement, leading in some cases to higher intensities than in the absence of surfactants. This is attributed to the transformation of the initially formed aggregates into some new aggregate species involving surfactant and polymer. These changes in HTMA-PFP fluorescence as a function of n-alkyl sulfonate concentration are important for the general understanding of polymer-surfactant interactions, and the aggregates formed may be important as novel systems for applications of these conjugated polyelectrolytes.

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Section: Resultssupporting

confidence: 71%

Modulating the Emission Intensity of Poly-(9,9-bis(6‘-N,N,N-trimethylammonium)hexyl)-Fluorene Phenylene) Bromide Through Interaction with Sodium Alkylsulfonate Surfactants

et al. 2007

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“…Los espectros de RMN de 1 H y 13 C se realizaron en un espectróme-tro Varian 400Mz, usando disolventes deuterados y los desplazamien-tos químicos se expresaron en partes por millón. Los espectros de FT-IR se registraron con un espectrofotómetro JascoFT/IR430 en muestras preparadas sobre bromuro potásico.…”

Section: Instrumentaciónunclassified

Películas fluorescentes azules basadas en derivados de poli-2,7-fluorenofenilideno

Mallavia¹,

Martínez-Pérez²,

Chmelka³

et al. 2004

Bol. Soc. Esp. Ceram. Vidr.

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La cuaternización del poli-(9,9-bis(6'-bromohexilo)fluorenofenileno) por tratamiento de trimetilamina gaseosa se emplea para obtener una sal de amonio del copolímero precursor soluble en agua. El copolímero neutro contiene unidades alternantes repetitivas de fluoreno/ fenilideno obtenidas mediante una acoplamiento de Suzuki con Pd (II). Los polímeros se caracterizaron por cromatografía de permeación (GPC), espectroscopia RMN de 1 H y 13 C , espectroscopia IR transformada de Fourier y análisis termogravimétrico (ATG). El polímero neutro mantiene la estabilidad hasta los 300ºC, mientras el derivado catiónico comienza a descomponer a 120ºC, con una progresiva perdida de masa hasta los 290ºC. Las propiedades ópticas de los polímeros se estudiaron en disolución y en películas mediante espectroscopias UV/VIS y de fluorescencia. Las películas delgadas de sílice mesoporosa se prepararon como materiales receptores de los copolímeros fluorescentes. Palabras clave: Polímeros conjugados, solubles en agua, Pd(II), sílice mesoporosa.Blue fluorescent films based on poly-2,7-fluorene-phenylene derivatives.Quaternization of poly-(9,9-bis(6'-bromohexyl)fluorenephenylene by treatment with trimethylamine gas was used to obtain a water soluble ammonium salt copolymer. The neutral copolymer containing fluorene/phenylene alternating repeating units was obtained by a palladiumcatalyzed Suzuki coupling reaction. This strategy could be applied to prepare water soluble conjugated polymers with the ability to change the charge functionality. The polymers were characterized by gel permeation chromatography (GPC), 1 H and 13 C NMR spectroscopy, FT-IR spectroscopy, and thermogravimetric analysis (TGA). The neutral polymer was stable to over 300ºC, while the cationic polymer begins to degrade at 120ºC with a progressive loss of mass at 290ºC. The optical properties of the polymers were investigated in solution and solid phases by UV/VIS and fluorescent spectroscopy. Mesoporous silica thin films were prepared as a host matrix for the fluorescent copolymers.

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“…Because the energy transfer process shows strong distance dependence, it is important to consider the relative length scales of the target DNA and cationic CP. Based on the molecular weight of the polymer (M w Ϸ 13,000 g͞mole, Ϸ13 Å per repeat unit), it is evident that the CP is longer than the oligonucleotide (15-28 base) target sequences tested previously (27)(28)(29)(30)(31)44).…”

Section: Resultsmentioning

confidence: 99%

“…This collective behavior gives rise to optical enhancement or amplification if the lowenergy site comprises a reporter fluorophore. The water solubility of these macromolecules is achieved by attaching ionic side chains pendent to the otherwise hydrophobic main chain, whereas the spectral properties are tuned by altering the actual conjugated backbone structure (26)(27)(28)(29)(30)(31).…”

mentioning

confidence: 88%

SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification

Gaylord

Massie

Feinstein

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

166

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A strategy employing a combination of peptide nucleic acid (PNA) probes, an optically amplifying conjugated polymer (CP), and S1 nuclease enzyme is capable of detecting SNPs in a simple, rapid, and sensitive manner. The recognition is accomplished by sequence-specific hybridization between the uncharged, fluorescein-labeled PNA probe and the DNA sequence of interest. After subsequent treatment with S1 nuclease, the cationic water soluble CP electrostatically associates with the remaining anionic PNA͞ DNA complex, leading to sensitized emission of the labeled PNA probe via FRET from the CP. The generation of fluorescent signal is controlled by strand-specific electrostatic interactions and is governed by the complementarity of the probe͞target pair. To assess the method, we compared the ability of the sensor system to detect normal, wild-type human DNA sequences, and those sequences containing a single base mutation. Specifically, we examined a PNA probe complementary to a region of the gene encoding the microtubule associated protein tau. The probe sequence covers a known point mutation implicated in a dominant neurodegenerative dementia known as frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17), which has clinical and molecular similarities to Alzheimer's disease. By using an appropriate PNA probe, the conjugated polymer poly[(9,9-bis(6 -N,N,Ntrimethylammoniumhexylbromide)fluorene)-co-phenylene] and S1 nuclease, unambiguous FRET signaling is achieved for the wildtype DNA and not the mutant sequence harboring the SNP. Distance relationships in the CP͞PNA assay are also discussed to highlight constraints and demonstrate improvements within the system. biosensor ͉ energy transfer ͉ hybridization probes ͉ polyelectrolyte ͉ fluorescence

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Energy Transfer in Mixtures of Water- Soluble Oligomers: Effect of Charge, Aggregation, and Surfactant Complexation

Cited by 197 publications

References 0 publications

Modulating the Emission Intensity of Poly-(9,9-bis(6‘-N,N,N-trimethylammonium)hexyl)-Fluorene Phenylene) Bromide Through Interaction with Sodium Alkylsulfonate Surfactants

Modulating the Emission Intensity of Poly-(9,9-bis(6‘-N,N,N-trimethylammonium)hexyl)-Fluorene Phenylene) Bromide Through Interaction with Sodium Alkylsulfonate Surfactants

Películas fluorescentes azules basadas en derivados de poli-2,7-fluorenofenilideno

SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification

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