Engineered Multifunctional Nanoparticles for DLA Cancer Cells Targeting, Sorting, MR Imaging and Drug Delivery

Amsaveni, Gunasekaran; Farook, Abubacker Sulaiman; Haribabu, Viswanathan; Murugesan, Ramachandran; Girigoswami, Agnishwar

doi:10.1166/asem.2013.1425

Cited by 33 publications

(20 citation statements)

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“…Daltons lymphoma ascites (DLA) cells were obtained from Kamaraj University, Madurai, India, and propagated into transplantable tumors in the peritoneal cavity and hind leg of female Swiss albino mice. DLA tumor was maintained by serial transplantation from mice to mice in the peritoneal cavity and solid tumor was maintained by injection of cell suspension in PBS on the right hind leg . DLA tumor was selected for the study because of their uniformity in growth.…”

Section: Methodsmentioning

confidence: 99%

“…The reduction in size of materials to nano scale leads to increase in surface area which in turn varies the optical and mechanical properties in remarkable way. In case of magnetic materials, size reduction to nanoscale makes these magnetic materials superparamagnetic in nature which has made them a choice to be applied for various biomedical applications . Magnetic nanoparticles have gained their importance in diagnosis and treatment of cancer and other such dreadful diseases .…”

Section: Introductionmentioning

confidence: 99%

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Optimized Mn‐doped iron oxide nanoparticles entrapped in dendrimer for dual contrasting role in MRI

et al. 2015

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Magnetic resonance imaging has acquired importance as a major tool for diagnosis and staging of cancers in humans. Injection of certain imaging agents have proved to improve contrast between normal and cancer cells on magnetic resonance imaging (MRI). Using the principles of MR contrast imaging, we have designed a dual mode (T1 and T2) contrast agent based on folic acid functionalized manganese ferrite nanoparticles (MNP) entrapped in 3G polyamidoamide (PAMAM) dendrimers. The ratio of Mn:Fe was tuned to achieve optimal performance. This multifunctional nanocarrier system was developed for targeting cancer cells to produce both T1 and T2 contrast which in turn helps in better diagnosis and staging of cancer. FTIR spectroscopy, X-Ray diffraction, atomic absorption spectroscopy, UV-Visible spectroscopy, and dynamic light scattering measurements were employed to characterize the multifunctional system at different stages of engineering. The ratio of relaxivities r2/r1 is 4.6 at 1.5 T for the MNP prepared with 0.5 molar ratio of Mn/Fe based on MR images obtained from phantom and tumor bearing mouse. The value of r2/r1 shows that the 0.5 molar ratio of Mn/Fe can be used to prepare MNP for the production of dual mode contrast in MR imaging.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimized Mn‐doped iron oxide nanoparticles entrapped in dendrimer for dual contrasting role in MRI

et al. 2015

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“…The protein expression levels of p-ERK1/2, ERK1/2 and GAPDH in the SH-SY5Y cells that were cultured on PLLA films (control group), aligned PLLA nanofibers (AF group) and aligned PLLA nanofibers with the cell culture medium in addition with 0.5 mg/mL pentapeptide GRGDS (GA group). engineering, [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38] it will be important to investigate the interaction mechanism between cells and materials fabricated by nanotechnologies. We believe that the results shown above might be helpful to guide the design and application of aligned nanofibers in regeneration medicine fields such as nerve tissue engineering.…”

Section: Effects Of Aligned Plla Nanofibers On Thementioning

confidence: 99%

The Interactions Between Aligned Poly(L-Lactic Acid) Nanofibers and SH-SY5Y Cells In Vitro

Meng

Man

et al. 2016

j nanosci nanotechnol

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Aligned nanofibers have been regarded as promising nanomaterials in facilitating nerve regeneration. Investigating the interactions between aligned nanofibers and neuronal cells will be critically important for the design and application of aligned nanofibers in nerve tissue engineering. In this study, we explored the effects of electrospun Poly(L-Lactic Acid) (PLLA) aligned nanofibers on SH-SY5Y cells (a type of human neuroblastoma cell line) and specifically focused on the role of integrin in the PLLA aligned nanofiber-SH-SY5Y cell interaction. We found that PLLA aligned nanofibers could significantly guide the neurite outgrowth of SH-SY5Y cell, and promote the viability, proliferation, glucose and lactic acid metabolism of SH-SY5Y cell. This promotion effect could be alleviated when the functions of integrins on the SH-SY5Y cell membrane were hampered by pentapeptide GRGDS. Moreover, we found that PLLA aligned nanofibers could enhance the expression of phosphorylated-ERK1/2 (p-ERK1/2) in the SH-SY5Y cells and blocking the integrins would decrease p-ERK1/2 expression. These results suggested that PLLA aligned nanofibers might affect many cellular behaviors of SH-SY5Y cells via integrin mediated ERK pathway. Our findings provided more insights for understanding the interaction between aligned nanofibers and neuronal cells.

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“…As the development of cancer therapy, theranostic treatment has been highlighted. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] Carbon nanoparticle in CNSI is a promising drug carrier for its huge surface area. 24 25 Combining with the lymph node targeting and mapping properties, CNSI is a potential carrier for theranostic of metastatic cancer.…”

Section: Introductionmentioning

confidence: 99%

Drug-Loaded Carbon Nanoparticle Suspension Injection: Drug Selection, Releasing Behavior, <I>In Vitro</I> Cytotoxicity and <I>In Vivo</I> Lymph Node Targeting

Xie¹,

Tang²,

Li³

et al. 2016

j nanosci nanotechnol

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In this study, drug-loaded carbon nanoparticle suspension as theranostic carrier was prepared for lymph node targeting delivery. Five model anti-cancer drugs were chosen to evaluate the drug selection, drug loading and releasing performance of carbon nanoparticle suspension. The relative recovery, precision, stability and limited detection range were studied in details. We found that the CNSI selectively adsorbed DOX and EPI and hardly adsorbed PTX, CDDP and 5-FU. The isothermal curves of CNSI adsorption to DOX and EPI are all fitted well with the Freundlich model. The adsorption process is not monolayer adsorption. By in vivo topical administration of DOX-loaded CNSI and EPI-loaded CNSI, we found that the drug-loaded CNSI were not only maintained its lymph node staining properties but also targeted delivery the drugs to the lymph node. The results demonstrated that the CNSI is not only used to vital staining of lymph vessels and lymph nodes but also can be used as drug delivery carrier. CNSI is a promising theranostic carrier for DOX or EPI delivery in cancer therapy.

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Engineered Multifunctional Nanoparticles for DLA Cancer Cells Targeting, Sorting, MR Imaging and Drug Delivery

Cited by 33 publications

References 0 publications

Optimized Mn‐doped iron oxide nanoparticles entrapped in dendrimer for dual contrasting role in MRI

Optimized Mn‐doped iron oxide nanoparticles entrapped in dendrimer for dual contrasting role in MRI

The Interactions Between Aligned Poly(L-Lactic Acid) Nanofibers and SH-SY5Y Cells In Vitro

Drug-Loaded Carbon Nanoparticle Suspension Injection: Drug Selection, Releasing Behavior, <I>In Vitro</I> Cytotoxicity and <I>In Vivo</I> Lymph Node Targeting

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