2012
DOI: 10.1128/aem.02171-12
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Engineering Filamentous Fungi for Conversion of d -Galacturonic Acid to l -Galactonic Acid

Abstract: D-Galacturonic acid, the main monomer of pectin, is an attractive substrate for bioconversions, since pectin-rich biomass is abundantly available and pectin is easily hydrolyzed. L-Galactonic acid is an intermediate in the eukaryotic pathway for D-galacturonic acid catabolism, but extracellular accumulation of L-galactonic acid has not been reported. By deleting the gene encoding L-galactonic acid dehydratase (lgd1 or gaaB) in two filamentous fungi, strains were obtained that converted D-galacturonic acid to L… Show more

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Cited by 46 publications
(53 citation statements)
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“…This value is relatively close to the initial productivities of 46–64 and 54–70 mg l −1  h −1 that were obtained with Δ gaaB and Δ gaaB - gaaA , respectively, in SmF of a defined medium supplemented with 10 g l −1 of D-galacturonic acid and 2 g l −1 of D-xylose (Kuivanen et al [2012]). The product yields (% of theoretical maximum, i.e.…”
Section: Discussionsupporting
confidence: 83%
See 1 more Smart Citation
“…This value is relatively close to the initial productivities of 46–64 and 54–70 mg l −1  h −1 that were obtained with Δ gaaB and Δ gaaB - gaaA , respectively, in SmF of a defined medium supplemented with 10 g l −1 of D-galacturonic acid and 2 g l −1 of D-xylose (Kuivanen et al [2012]). The product yields (% of theoretical maximum, i.e.…”
Section: Discussionsupporting
confidence: 83%
“…Since this strain produces pectinases, it was able to produce L-galactonate from polygalacturonate in a submerged fermentation (Kuivanen et al [2012]). The aim of the current work was to determine whether this strain could be used to hydrolyse the pectin in the CPW and convert the resulting D-galacturonic acid to L-galactonic acid in a single fermentation process.…”
Section: Introductionmentioning
confidence: 99%
“…The genes encoding these enzymes (gaaA, gaaB, gaaC, and gaaD have been identified and the biochemical properties of the enzymes have been determined (Kuorelahti et al, 2005Hilditch et al, 2007;Mojzita et al, 2010;Wiebe et al, 2010;Liepins et al, 2006;Zhang et al, 2011;Kuivanen et al, 2012). Specific sugar transporters that are able to transport GA over the plasma membrane have recently been identified and characterised in Neurospora crassa (Benz et al, 2014) as well as in A. niger (Sloothaak et al, 2014) and Botrytis cinerea (Zhang et al, 2014).…”
Section: Introductionmentioning
confidence: 97%
“…library.cornell.edu/usda/current/CropProdSu/CropProdSu-01-10-2014.pdf). Pectins are currently used primarily as animal feed or discarded; the use as livestock feed involves drying the pulp to preclude spoilage, with an attendant energy requirement 30-40% of the total energy input for beet processing [1,2], while disposal incurs added expense, and it is thus becoming economically and environmentally desirable to develop value-added products from these bio-resources. Structurally, pectins comprise a family of heterogeneous polysaccharides from plant primary cell walls wherein d-galacturonic acid (GalUA) occurs in abundance, and they can be grouped as homogalacturonan (HG), xylogalacturonan, rhamnogalacturonan I, and rhamnogalacturonan II [3].…”
Section: Introductionmentioning
confidence: 99%