Engineering of Adipose Tissue by Injection of Human Preadipocytes in Fibrin

Torio-Padron, Nestor; Baerlecken, N.T.; Momeni, Arash; Stark, G. B.; Borges, J.

doi:10.1007/s00266-006-0221-6

Cited by 98 publications

(70 citation statements)

References 27 publications

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“…Adipogenic differentiation did not significantly affect the hLCAT delivery and the cell survival in this model using fibrin glue as a scaffold with m-ccdPA ( Figure 2B). In this context, our results may suggest that the transplanted cells with fibrin glue were differentiated into adipocytes without adipogenic pretreatment (Cho et al, 2006;Torio-Padron et al, 2007a). The immunohistochemical observation did not clearly show that ccdPA would undergo adipogenic differentiation after transplantation, but the transplanted ccdPA were clearly identified as hLCAT-delivery cells in the transplanted sites of the recipient mice ( Figures 3A-3F).…”

Section: Discussionmentioning

confidence: 77%

Fibrin glue increases the cell survival and the transduced gene product secretion of the ceiling culture-derived adipocytes transplanted in mice

et al. 2011

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The development of clinically applicable scaffolds is important for the application of cell transplantation in various human diseases. The aims of this study are to evaluate fibrin glue in a novel protein replacement therapy using proliferative adipocytes and to develop a mouse model system to monitor the delivery of the transgene product into the blood and the fate of the transduced cells after transplantation. Proliferative adipocytes from mouse adipose tissue were transduced by a retroviral vector harboring the human lecithin-cholesterol acyltransferase (lcat) gene, and were subcutaneously transplanted into mice combined with fibrin glue. The lcat gene transduction efficiency and the subsequent secretion of the product in mouse adipocytes were enhanced using a protamine concentration of 500 μ g/ml. Adipogenesis induction did not significantly affect the lcat gene-transduced cell survival after transplantation. Immunohistochemistry showed the ectopic enzyme production to persist for 28 days in the subcutaneously transplanted genetransduced adipocytes. The increased viability of transplanted cells with fibrin glue was accompanied with the decrease in apoptotic cell death. The immunodetectable serum LCAT levels in mice implanted with the fibrin glue were comparable with those observed in mice implanted with Matrigel, indicating that the transplanted lcat gene-transduced adipocytes survived and functioned in the transplanted spaces with fibrin glue as well as with Matrigel for 28 days. Thus, this in vivo system using fibrin is expected to serve as a good model to further improve the transplanted cell/scaffold conditions for the stable and durable cell-based replacement of defective proteins in patients with LCAT deficiency.

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Section: Discussionmentioning

confidence: 77%

Fibrin glue increases the cell survival and the transduced gene product secretion of the ceiling culture-derived adipocytes transplanted in mice

et al. 2011

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“…Im Falle eines ausgedehnten Defektes im Gesichtsbereich bietet sich die allogene Gesichtstransplantation als potenzielle operative Lösung an, nicht zuletzt weil das "tissue engineering" [23] und die Stammzellenforschung [13] bisher keine echte klinische Alternative bieten.…”

Section: Diskussionunclassified

Allogene Gesichtstransplantation: Fiktion oder Realität?

Messmer

Baccarani

Follmar

et al. 2007

Chirurg

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“…Isolation of pre-adipocytes was performed directly after harvesting of the materials as described before [Torio-Padron et al, 2007]. To isolate pre-adipocytes from excised tissue, fibrous structures and visible blood vessels were removed, tissue was minced into small pieces using a scalpel and washed in PBS.…”

Section: Pre-adipocyte Isolationmentioning

confidence: 99%

“…Isolated cells were differentiated into adipocytes using a differentiation medium based on Dulbecco's modified Eagle's medium/Ham's F-12 medium 1: 2 [Torio- Padron et al, 2007]. This medium was mixed with 3% fetal bovine serum, insulin 100 n M (Sigma, Deisenhofen, Germany), dexamethasone 1 M (Sigma), and, for the first 4 days of differentiation, methylisobutylxanthine 0.2 m M (Sigma).…”

Section: Adipogenic Differentiation and Oil Red O Assaymentioning

confidence: 99%

Comparison of Pre-Adipocyte Yield, Growth and Differentiation Characteristics from Excised versus Aspirated Adipose Tissue

Torio-Padron

Huotari

Eisenhardt

et al. 2010

Cells Tissues Organs

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Adipose tissue precursor cells (pre-adipocytes) are part of a stromal vascular fraction that can be easily isolated from fat tissue. Adipose tissue can be harvested by 2 methods: aspiration and excision. We analyzed whether the pre-adipocyte yield, growth characteristics and ability to differentiate into mature adipose tissue are influenced by the type of harvesting procedure. Adipose tissue was simultaneously harvested from the abdomen by surgical excision or aspiration according to the Coleman procedure in 10 individuals. This permitted inter- and intra-individual comparisons. Cell viability and yield were determined directly after isolation of pre-adipocytes. The growth kinetics were investigated in culture. Furthermore, pre-adipocytes were cultured under adipogenic conditions to compare their differentiation potential. The number of viable pre-adipocytes was significantly higher after excision of adipose tissue compared to aspiration. The proliferation kinetic was not influenced by the type of harvesting. No differences were observed in the differentiation potential of the pre-adipocytes between both groups. Compared to excision, aspiration of adipose tissue negatively affects the yield of pre-adipocytes. However, growth characteristics and differentiation potential of viable cultured cells are not influenced by the type of surgical harvesting. Due to its reduced donor site morbidity, we conclude that aspiration of adipose tissue is a valid harvesting method for isolation of pre-adipocytes.

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Engineering of Adipose Tissue by Injection of Human Preadipocytes in Fibrin

Cited by 98 publications

References 27 publications

Fibrin glue increases the cell survival and the transduced gene product secretion of the ceiling culture-derived adipocytes transplanted in mice

Fibrin glue increases the cell survival and the transduced gene product secretion of the ceiling culture-derived adipocytes transplanted in mice

Allogene Gesichtstransplantation: Fiktion oder Realität?

Comparison of Pre-Adipocyte Yield, Growth and Differentiation Characteristics from Excised versus Aspirated Adipose Tissue

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