Engineering RGD nanopatterned hydrogels to control preosteoblast behavior: A combined computational and experimental approach

Comisar, Wendy; Kazmers, Nikolas H.; Mooney, David J.; Linderman, Jennifer J.

doi:10.1016/j.biomaterials.2007.06.018

Cited by 134 publications

(114 citation statements)

References 40 publications

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“…Because the mechanical rigidity of alginate-based adhesion substrates may be readily modulated, these synthetic ECMs may also be valuable in evaluating the role of altered mechanical stiffness of the tumor stroma in cancer progression. Furthermore, alginate-based artificial ECMs may be readily varied with regard to their bulk density and patterns of RGD peptide presentation while maintaining constant physicochemical properties, allowing for investigations of tumor vascularization and progression as a function of these parameters (33). Insights gained by these studies may improve our understanding of cancer and ultimately contribute to the development of more efficient anticancer therapies.…”

Section: Resultsmentioning

confidence: 99%

Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement

Fischbach

Kong

Hsiong

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

278

210

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Three-dimensional culture alters cancer cell signaling; however, the underlying mechanisms and importance of these changes on tumor vascularization remain unclear. A hydrogel system was used to examine the role of the transition from 2D to 3D culture, with and without integrin engagement, on cancer cell angiogenic capability. Threedimensional culture recreated tumor microenvironmental cues and led to enhanced interleukin 8 (IL-8) secretion that depended on integrin engagement with adhesion peptides coupled to the polymer. In contrast, vascular endothelial growth factor (VEGF) secretion was unaffected by 3D culture with or without substrate adhesion. IL-8 diffused greater distances and was present in higher concentrations in the systemic circulation, relative to VEGF. Implantation of a polymeric IL-8 delivery system into GFP bone marrow-transplanted mice revealed that localized IL-8 up-regulation was critical to both the local and systemic control of tumor vascularization in vivo. In summary, 3D integrin engagement within tumor microenvironments regulates cancer cell angiogenic signaling, and controlled local and systemic blockade of both IL-8 and VEGF signaling may improve antiangiogenic therapies.ECM ͉ microenvironment ͉ tumor vascularization ͉ drug delivery ͉ IL-8

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Section: Resultsmentioning

confidence: 99%

Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement

Fischbach

Kong

Hsiong

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

278

210

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“…Earlier literature cites many applications of SA in agricultural applications after crosslinking with glutaraldehyde (GA) [13][14][15]. SA is one of the most versatile natural materials known to form hydrogels and films [16][17][18][19][20][21][22]. Drug-loaded films of SA are being used in pharmaceutical applications.…”

Section: Introductionmentioning

confidence: 99%

Sodium alginate/poly (ethylene oxide) blend hydrogel membranes for controlled release of valganciclovir hydrochloride

Mallikarjuna

Rao

Siraj

et al. 2012

Designed Monomers and Polymers

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Sodium alginate (SA) and poly (ethylene oxide) PEO blend membranes were prepared by solvent casting method for the controlled release of valganciclovir hydrochloride, an antiHIV drug. The prepared membranes were thin, flexible, smooth, and characterized by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), X-ray diffraction (X-RD), scanning electron microscopy (SEM), and tensile strength measurement. FTIR was used to understand the formation of hydrogen bonding between SA and PEO. The DSC and X-RD studies were performed to understand the crystalline nature of drug after encapsulation into the membranes. SEM was used to study the surface morphology of the membranes. Tensile strength measurements revealed the mechanical strength of the membranes. In vitro release studies indicated a dependence of release rate on the extent of crosslinking, amount of drug loading, and the amount of PEO, but slow release rates was extended up to 12 h. Cumulative release data were fitted onto to an empirical equation to compute diffusional exponent (n), which indicated the nonFickian trend for drug release.

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“…This scaffold provides a structural and logistical template for developing tissue, which can markedly affect cell behavior. The properties of scaffolds that are important for bone formation include the size, distribution and shape of the pores, the surface roughness, the presence of cell attachment sites and the biomechanics of both the material and the scaffold structures [14][15][16][17] . The most suitable scaffolds for bone formation are those made of osteoconductive materials, such as bone proteins and HA, with mechanical properties similar to those of load-bearing native bone that stimulate osteogenesis and have large and interconnected pores to facilitate cell infiltration and matrix deposition, and rough inner surfaces to promote cell attachment.…”

Section: Scaffoldsmentioning

confidence: 99%

Adipose mesenchymal stem cells in the field of bone tissue engineering

Romagnoli

Brandi

2014

WJSC

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Engineering RGD nanopatterned hydrogels to control preosteoblast behavior: A combined computational and experimental approach

Cited by 134 publications

References 40 publications

Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement

Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement

Sodium alginate/poly (ethylene oxide) blend hydrogel membranes for controlled release of valganciclovir hydrochloride

Adipose mesenchymal stem cells in the field of bone tissue engineering

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