Engineering the Nanoparticle-Protein Interface for Cancer Therapeutics

Saie, Amir Ata; Ray, Moumita; Rotello, Vincent M.

doi:10.1007/978-3-319-16555-4_11

Cited by 23 publications

(15 citation statements)

References 125 publications

(130 reference statements)

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“…Additionally, more work is needed to ensure predictable biological and in vivo outcomes (Azhdarzadeh et al, 2015a; Mahon et al, 2012). Should this goal be accomplished, it could potentially revolutionize multiple therapeutic areas and make significant improvements in nanomedicine (Saie et al, 2015). …”

Section: Challenges Of the Protein Coronamentioning

confidence: 99%

“…In the presence of the corona, cells are protected from the damaging effects that the bare nanoparticle surface can engender until the nanoparticles and protein corona are cleared through phagocytosis (Wang et al, 2013). Understanding these fundamental processes are essential as the unique combination of the nano-bio interphase directs the nature of a corona, which then determines the biological identity of the protein corona-nanoparticle complex (Saie et al, 2015; Jiang et al, 2010). …”

Section: Introductionmentioning

confidence: 99%

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Protein corona: Opportunities and challenges

Zanganeh

Spitler

Erfanzadeh

et al. 2016

The International Journal of Biochemistry & Cell Biology

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154

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In contact with biological fluids diverse type of biomolecules (e.g., proteins) adsorb onto nanoparticles forming protein corona. Surface properties of the coated nanoparticles, in terms of type and amount of associated proteins, dictate their interactions with biological systems and thus biological fate, therapeutic efficiency and toxicity. In this perspective, we will focus on the recent advances and pitfalls in the protein corona field.

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Section: Challenges Of the Protein Coronamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein corona: Opportunities and challenges

Zanganeh

Spitler

Erfanzadeh

et al. 2016

The International Journal of Biochemistry & Cell Biology

Self Cite

154

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“…The formation of protein corona has been experimentally interpreted as competitive adsorption and desorption of plasma proteins on particle surfaces, called the Vroman effect . Lundqvist et al found that the adsorption of proteins onto polystyrene (PS) nanoparticles is modulated by particle size and surface properties, which is rationalized by van der Waals, electrostatic and hydrophobic interactions . Among those forces, the hydrophobic interaction has been considered as a key factor for protein adsorption .…”

Section: Introductionmentioning

confidence: 99%

Effects of Nanoparticle Electrostatics and Protein–Protein Interactions on Corona Formation: Conformation and Hydrodynamics

Lee

2020

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All‐atom molecular dynamics simulations of plasma proteins (human serum albumin, fibrinogen, immunoglobulin gamma‐1 chain‐C, complement C3, and apolipoprotein A‐I) adsorbed onto 10 nm sized cationic, anionic, and neutral polystyrene (PS) particles in water are performed. In simulations of a single protein with a PS particle, proteins eventually bind to all PS particles, regardless of particle charge, in agreement with experiments showing the binding between anionic proteins and particles, which is further confirmed by calculating the binding free energies from umbrella sampling simulations. Simulations of mixtures of multiple proteins and a PS particle show the formation of the protein layer on the surface via the adsorption competition between proteins, which influences the binding affinity and structure of adsorbed proteins. In particular, diffusivities are much higher for proteins bound to the particle surface or to the boundary of the protein layer than for those bound to both the particle surface and other proteins, indicating the dependence of protein mobility on their positions in the layer. These findings help to explain in detail experimental observations regarding the replacement of plasma proteins at the early stage of corona formation and the difference in the binding strength of proteins in inner and outer protein‐layers.

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“…Our previous research on nano/micro-particle systems composed of HAp coated with the chitosan-poly(D,L)-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) has justified the use of HAp as a core component of composite drug carriers for organ-targeting therapies [19]. Along with the particle size, shape and composition, the fate of particles injected into the bloodstream is mainly defined by the type and the structure of proteins bound to them [20, 21], which can be controlled by tailoring the composition, the topography and the charge distribution on the particle surface. Coating HAp nanoparticles with different polymers has correspondingly directed HAp to different organs [19].…”

Section: Introductionmentioning

confidence: 99%

Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor

Ignjatović

Penov-Gaši

et al. 2016

Colloids and Surfaces B: Biointerfaces

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In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47 wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50 = 168 nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.

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Engineering the Nanoparticle-Protein Interface for Cancer Therapeutics

Cited by 23 publications

References 125 publications

Protein corona: Opportunities and challenges

Protein corona: Opportunities and challenges

Effects of Nanoparticle Electrostatics and Protein–Protein Interactions on Corona Formation: Conformation and Hydrodynamics

Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor

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