Engineering Three-Dimensional Pulmonary Tissue Constructs

Mondrinos, Mark J.; Koutzaki, Sirma H.; Jiwanmall, Eugean; Li, Mengyan; Dechadarevian, Jean-Pierre; Lelkes, Peter I.; Finck, Christine

doi:10.1089/ten.2005.12.ft-1

Cited by 28 publications

(45 citation statements)

References 23 publications

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“…Fetal pulmonary cells were isolated by a previously described method [9]. Briefly, surgically harvested fetal lungs were rinsed in HBSS and digested with 0.05% trypsin in PBS for 30min.…”

Section: Methodsmentioning

confidence: 99%

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Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels

Nalayanda

Wang

Fulton

et al. 2010

Journal of Pediatric Surgery

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Introduction-Pulmonary hypoplasia is a condition of the newborn that is characterized by underdeveloped lungs and poor outcome. One strategy in the treatment of patients with hypoplasia is to augment underdeveloped lungs using biocompatible artificial lung tissue. However, one central challenge in current pulmonary tissue engineering efforts remains the development of a stable biomimetic alveolar-capillary membrane. Accordingly, we have built a series of bio-mimetic microfluidic devices that specifically model the alveolar-capillary membrane. Current designs include a single-layer microchip that exposes alveolar and endothelial cell types to controlled fluidic stimuli. A more advanced multi-layered device allows for alveolar cells to be cultured at an airinterface while allowing constant media nourishment and waste removal, thus better mimicking the physiologic milieu of the alveolar-capillary interface. Both devices possess the benefit of parallel testing.

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“…Fetal pulmonary cells were isolated by a previously described method [9]. Briefly, surgically harvested fetal lungs were rinsed in HBSS and digested with 0.05% trypsin in PBS for 30min.…”

Section: Methodsmentioning

confidence: 99%

“…Pulmonary hypoplasia is responsible for more than 2800 deaths annually in the United States [1] with preterm birth being a major contributor. It is a condition in neonates characterized by reduced lung mass and deficient surfactant production from inadequately differentiated alveolar epithelium, leading to an impeded alveolar gas exchange [2].…”

mentioning

confidence: 99%

Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels

Nalayanda

Wang

Fulton

et al. 2010

Journal of Pediatric Surgery

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“…Synthetic polymers can be produced with a wide range of mechanical and chemical properties [8]. Degradable synthetic matrices that have been used to engineer lung tissue include polyglycolic acid (PGA) in the form of a felt sheet [9], PGA combined with pluronic F-127 [9], polylactic-co-glycolic acid (PLGA) [9] or poly-L-lactic-acid (PLLA) [9,10], poly-DL-lactic acid (PDLLA) [10], and polydimethylsiloxane [11].…”

Section: Scaffolds For Lung Engineeringmentioning

confidence: 99%

“…Changes in ECM structure and composition could influence cell adhesion and provide critical cues that orchestrate tissue formation and cell function [88]. There have been a few reports supporting a role for lung-specific scaffold or at least cellÀECM interactions in influencing differentiation of stem or progenitor cells [10,32,86,87]. Early studies suggested that specific components of ECM materials could strongly influence the differentiation of ESC into pneumocytes [32].…”

Section: Role Of Ac Matrix In Lung Lineage Differentiationmentioning

confidence: 99%

Lung Regeneration

Nichols

Cortiella

2014

Regenerative Medicine Applications in Organ Transplantation

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“…Prior attempts at pulmonary tissue engineering have been limited to small tissue sizes that permit nutrient delivery to the growing tissues via diffusion (1,7,8,19,20), although recently described work demonstrates that larger tissues can be developed (9,24). In work by Cortiella and colleagues, engineered lung culture relied solely on diffusion, with no vascular perfusion or airway ventilation (9), while Price and co-workers provided ventilation of developing engineered tissues but no vascular perfusion (24).…”

Section: Introductionmentioning

confidence: 99%

Bioreactor for the Long-Term Culture of Lung Tissue

et al. 2011

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In this paper, we describe the design and validation of a bioreactor for the in vitro culture of whole rodent lung tissue. Many current systems only enable large segments of lung tissue to be studied ex vivo for up to a few hours in the laboratory. This limitation restricts the study of pulmonary biology in controlled laboratory settings, and also impacts the ability to reliably culture engineered lung tissues in the laboratory. Therefore, we designed, built and validated a bioreactor intended to provide sufficient nutrient supply and mechanical stimulation to support cell survival and differentiation in cultured lung tissue. We also studied the effects of perfusion and ventilation on pulmonary cell survival and maintenance of cell differentiation state. The final bioreactor design described herein is capable of supporting the culture of whole native lung tissue for up to 1 week in the laboratory, and offers promise in the study of pulmonary biology and the development of engineered lung tissues in the laboratory.

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Engineering Three-Dimensional Pulmonary Tissue Constructs

Cited by 28 publications

References 23 publications

Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels

Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels

Lung Regeneration

Bioreactor for the Long-Term Culture of Lung Tissue

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