Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy

Ji, Xiao‐Jun; Huang, He; Du, Jun; Zhu, Jian-Guo; Ren, Lu‐Jing; Hu, Nan; Li, Shuang

doi:10.1016/j.biortech.2009.02.031

Cited by 131 publications

(98 citation statements)

References 24 publications

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“…K. oxytoca is used in production of 2,3-butylene glycol and 2,3-butanediol from xylose (4,5). Many studies of 2,3-butanediol production using K. oxytoca have been recently reported (6,7,11). In order to understand these unique properties on the genomic level, K. oxytoca KCTC 1686 was obtained from the Korean Collection for Type Culture (Daejun, South Korea).…”

mentioning

confidence: 99%

Complete Genome Sequence of Klebsiella oxytoca KCTC 1686, Used in Production of 2,3-Butanediol

Shin

Kim

et al. 2012

J Bacteriol

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confidence: 99%

Complete Genome Sequence of Klebsiella oxytoca KCTC 1686, Used in Production of 2,3-Butanediol

Shin

Kim

et al. 2012

J Bacteriol

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“…The 16S rDNA analysis proved that strain SWU-27 was a member of K. pneumoniae and the results of morphological, biochemical and physiological tests, obeying to the Bergey's Manual of Systematic Bacteriology, confirmed these results. In previous studies, K. oxytoca have the phenol-degrading activity and have been certified to produce ethanol and 2, 3-butanediol from xylose and glucose (Ji et al, 2009). The mechanism and improvement of producing 1,3-propanediol and extended spectrum beta-lactamases by K. pneumoniae had become the main focuses of the previous studies (Menzel et al, 1997;Pena et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Isolation of a Thermostable Alkaline Cellulase-producing Bacterium Strain from a Garbage Dump

Zhen-gang¹,

Tang²,

Lan³

et al. 2015

IJAB

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Thermostable alkaline cellulases can resist high temperature and high pH, which make them widely applicable in the industries of food, detergent, paper making, and biomass utilization. To obtain favorite thermostable alkaline cellulaseproducing bacterial strains, these bacteria were isolated from collected soil and plant putrefaction samples in long-term garbage dumps. One bacterium strain from lime-applied garbage dumps, named as SWU-27, was picked up since its crude enzyme showed stronger activities of cellubiohydrolase, endoglucanase, and β-glucosidase. All these activities could tolerate the treatment of high temperature (up to 80°C) and endoglucanase and β-glucosidase activities could even tolerate high pH (over 10). Taxonomic study showed that strain SWU-27 is another geographic strain of Gram-negative Klebsiella pneumoniae. In conclusion it is suggested that lime-applied dumps are preferable places to isolate novel thermostable alkaline cellulaseproducing bacterial strains.

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“…Therefore, the agitation speed was first controlled at 300 rpm to achieve higher biomass and carbon flux into 2,3-BD pathway, and subsequently it was switched to 200 rpm to reduce the AC accumulation. Higher 2,3-BD yield might be achieved in comparison to single agitation speed control conditions as described previously (Ji et al 2009). …”

Section: Batch Fermentations With Various Agitation Speedsmentioning

confidence: 97%

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

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Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

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Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy

Cited by 131 publications

References 24 publications

Complete Genome Sequence of Klebsiella oxytoca KCTC 1686, Used in Production of 2,3-Butanediol

Complete Genome Sequence of Klebsiella oxytoca KCTC 1686, Used in Production of 2,3-Butanediol

Isolation of a Thermostable Alkaline Cellulase-producing Bacterium Strain from a Garbage Dump

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

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