Enhanced activity of lysozyme-AgNP conjugate with synergic antibacterial effect without damaging the catalytic site of lysozyme

Ernest, Vinita; Sekar, Gajalakshmi; Mukherjee, Amitava; Chandrasekaran, Natarajan

doi:10.3109/21691401.2013.818010

Cited by 31 publications

(19 citation statements)

References 43 publications

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“…For instance, exposure at 25 μg/ml of AgNPs (6-20 nm in size) induced chromosomal aberrations in 10 % of the IMR-90 normal cell line and in 20 % of the U251 cancer cell line. The DNA damage, chromosomal aberrations, and cell cycle arrest raise the concern [35]. The dose response shows an increase in DNA damaging effect with higher the treatment nanoparticle concentration.…”

Section: Discussionmentioning

confidence: 99%

Increased DNA Damage and Oxidative Stress Among Silver Jewelry Workers

Aktepe

Koçyiğit

Yükselten

et al. 2015

Biol Trace Elem Res

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Silver has long been valued as a precious metal, and it is used to make ornaments, jewelry, high-value tableware, utensils, and currency coins. Human exposures to silver and silver compounds can occur oral, dermal, or by inhalation. In this study, we investigated genotoxic and oxidative effects of silver exposure among silver jewelry workers. DNA damage in peripheral mononuclear leukocytes was measured by using the comet assay. Serum total antioxidative status (TAS), total oxidative status (TOS), total thiol contents, and ceruloplasmin levels were measured by using colorimetric methods among silver jewelry workers. Moreover, oxidative stress index (OSI) was calculated. Results were compared with non-exposed healthy subjects. The mean values of mononuclear leukocyte DNA damage were significantly higher than control subjects (p < 0.001). Serum TOS, OSI, and ceruloplasmin levels were also found to be higher in silver particles exposed group than those of non-exposed group (p < 0.001, p < 0.001, p < 0.01, respectively). However, serum TAS levels and total thiol contents of silver exposed group were found significantly lower (p < 0.05, p < 0.001, respectively). Exposure to silver particles among silver jewelry workers caused oxidative stress and accumulation of severe DNA damage.

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Section: Discussionmentioning

confidence: 99%

Increased DNA Damage and Oxidative Stress Among Silver Jewelry Workers

Aktepe

Koçyiğit

Yükselten

et al. 2015

Biol Trace Elem Res

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“…To overcome this problem, SNPs have been synthesized using various biological moieties including antibiotics, enzymes, polysaccharides, and oligopeptides so as to enhance the antibacterial potential. [9][10][11] In recent years, antimicrobial peptides (AMPs) have emerged as excellent antimicrobial agents to address multidrug resistance in bacteria. 12,13 AMPs act like a "molecular knife" and facilitate the damage to bacterial cell membrane even at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Lambadi¹,

Sharma²,

Kumar³

et al. 2015

IJN

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Infectious diseases cause a huge burden on healthcare systems worldwide. Pathogenic bacteria establish infection by developing antibiotic resistance and modulating the host’s immune system, whereas opportunistic pathogens like Pseudomonas aeruginosa adapt to adverse conditions owing to their ability to form biofilms. In the present study, silver nanoparticles were biofunctionalized with polymyxin B, an antibacterial peptide using a facile method. The biofunctionalized nanoparticles (polymyxin B-capped silver nanoparticles, PBSNPs) were assessed for antibacterial activity against multiple drug-resistant clinical strain Vibrio fluvialis and nosocomial pathogen P. aeruginosa . The results of antibacterial assay revealed that PBSNPs had an approximately 3-fold higher effect than the citrate-capped nanoparticles (CSNPs). Morphological damage to the cell membrane was followed by scanning electron microscopy, testifying PBSNPs to be more potent in controlling the bacterial growth as compared with CSNPs. The bactericidal effect of PBSNPs was further confirmed by Live/Dead staining assays. Apart from the antibacterial activity, the biofunctionalized nanoparticles were found to resist biofilm formation. Electroplating of PBSNPs onto stainless steel surgical blades retained the antibacterial activity against P. aeruginosa . Further, the affinity of polymyxin for endotoxin was exploited for its removal using PBSNPs. It was found that the prepared nanoparticles removed 97% of the endotoxin from the solution. Such multifarious uses of metal nanoparticles are an attractive means of enhancing the potency of antimicrobial agents to control infections.

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“…Generally, it is possible to change the optimal pH, temperature and kinetic parameters for an immobilized enzyme as a result of the immobilized method, support structure and conformation change of enzyme after bounded, making the catalytic site more or less accessible to the substrate [32,33]. The effect of pH on activity of soluble HRP and immobilized HRP was evaluated by incubating these preparations in the buffers of varying pH values ranged from 4.0 to 9.0 ( Figure 6).…”

Section: Resultsmentioning

confidence: 99%

Immobilization of horseradish peroxidase on PMMA nanofibers incorporated with nanodiamond

Al-Shawafi

Aldhahri

Almulaiky

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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In the present study, nanodiamond (ND) was blended with polymethyl methacrylate (PMMA) and then electrospun into nanofibers (nfPMMA-ND) for the immobilization of horseradish peroxidase (HRP). The maximum immobilization efficiency of HRP (96%) was detected at 10% ND and pH 7.0. ATR-FTIR, SEM and TEM were used to characterize the immobilized enzyme. The immobilized enzyme retained 60% of its initial activity after ten reuses. The pH was shifted from 7.0 for soluble HRP to 7.5 for the immobilized enzyme. The soluble HRP had an optimum temperature of 30 C, whereas this temperature was shifted to 40 C for the immobilized enzyme. The substrate analogs were oxidized by immobilized HRP with higher efficiencies than those of soluble HRP. The kinetic results showed that the soluble HRP had more affinity toward guiacol and H 2 O 2 than immobilized HRP. The effect of metal ions on soluble and immobilized HRP was studied. The immobilized HRP was markedly more stable when it exposed to urea, isopropanol, butanol and heptane compared with the soluble enzyme. The immobilized HRP exhibited high resistance to proteolysis by trypsin than that of soluble enzyme. In conclusion, the nfPMMA-ND-HRP could be employed in several applications such as biosensor, biomedical and bioremediation.

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Enhanced activity of lysozyme-AgNP conjugate with synergic antibacterial effect without damaging the catalytic site of lysozyme

Abstract: In summary, the immobilization of lysozyme on AgNP has yielded a nano-bio-conjugate with synergistic antibacterial properties.

Cited by 31 publications

References 43 publications

Increased DNA Damage and Oxidative Stress Among Silver Jewelry Workers

Increased DNA Damage and Oxidative Stress Among Silver Jewelry Workers

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Immobilization of horseradish peroxidase on PMMA nanofibers incorporated with nanodiamond

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