Enhanced Amelioration of High-Fat Diet-Induced Fatty Liver by Docosahexaenoic Acid and Lysine Supplementations

Abstract

Fatty liver disease is the most common pathological condition in the liver. Here, we generated high-fat diet-(HFD-) induced nonalcoholic fatty liver disease (NAFLD) in mice and tested the effects of docosahexaenoic acid (DHA) and lysine during a four-week regular chow (RC)feeding. Our results showed that 1% lysine and the combination of 1% lysine + 1% DHA reduced body weight. Moreover, serum triglyceride levels were reduced by 1% DHA and 1% lysine, whereas serum alanine transaminase activity was reduced by 1% DHA and 1% DHA + 0.5% lysine. Switching to RC reduced hepatic lipid droplet accumulation, which was further reduced by the addition of DHA or lysine. Furthermore, the mRNA expressions of hepatic proinflammatory cytokines were suppressed by DHA and combinations of DHA + lysine, whereas the mRNA for the lipogenic gene, acetyl-CoA carboxylase 1 (ACC1), was suppressed by DHA. In the gonadal adipose tissues, combinations of DHA and lysine inhibited mRNA expression of lipid metabolism-associated genes, including ACC1, fatty acid synthase, lipoprotein lipase, and perilipin. In conclusion, the present study demonstrated that, in conjunction with RC-induced benefits, supplementation with DHA or lysine further ameliorated the high-fat diet-induced NAFLD and provided an alternative strategy to treat, and potentially prevent, NAFLD.

WITHDRAWN: Ginkgolide B reduces hepatic lipid accumulation and ameliorates nonalcoholic fatty liver disease in high-fat diet–induced obese mice

“…Many studies have found that purified compounds of plants or fish may improve NAFLD [24][25][26][27]. For example, 6-gingerol is linked to reduced body weight and inhibition of lipid accumulation of hepatocytes in obese mice [28].…”

Effects of dietary fishmeal replacement with Spirulina platensis on the growth, feed utilization, digestion and physiological parameters in juvenile gibel carp (Carassis auratus gibelio var. CAS III)

“…Meanwhile, the lipid content of whole body in the SP100 + Lys and SP100 + P groups further decreased compared to the SP100 group. It has been reported that supplementation of lysine could improve the utilization of amino acids and lead to less lipid storage (Lin et al., ). The declined lipid content of whole body in the SP100 + P group was probably due to that phosphorus accelerated mitochondrial β‐oxidation and reduced the lipid accumulation in fish (Roy & Lall, ).…”

Fish oil alleviated high-fat diet–induced non-alcoholic fatty liver disease via regulating hepatic lipids metabolism and metaflammation: a transcriptomic study

“…Additional file 1 : Table S1 lists the nutrient composition, energy ratio, and fatty acid compositions in different feedstuff. At the end of 16th week of the study, 12-hour fasted rats were sacrificed with CO 2 suffocate[ 42 ]. Blood was collected into a heparinized tube (6–8 mL) and centrifuged at 1000 × g for 15 min at 4 °C, and plasma was collected and stored at −80 °C until analysis.…”