Enhanced Antibacterial Activity of Poly (dimethylsiloxane) Membranes by Incorporating SiO2 Microspheres Generated Silver Nanoparticles

Abstract

The nonspecific adsorption of proteins and bacteria on the surface of polydimethylsiloxane (PDMS) had been a serious concern in a wide range of applications, such as medical devices. In order to improve the anti-adhesive and antibacterial capability, bare silver nanoparticles (AgNPs, ~15 nm) were generated in-situ on their surface without extra reducing and stabilizing agents. The main reason for this was that the SiO2 microspheres that are covalent bonded to the bulked PDMS could not only generate AgNPs spontaneously but also insure that no AgNPs were released to the environment. Meanwhile, the thiol-group-functionalized SiO2 microspheres self-assembled on the surface of PDMS by thiol-vinyl click reaction without any impact on their biomedical applications. After the modification of SiO2 microspheres with AgNPs, the surface of PDMS showed a smaller water contact angle than before, and the adhesion and growth of E. coli and Bacillus subtilis were effectively inhibited. When the monolayer of SiO2 microspheres with AgNPs was assembled completely on the surface of PDMS, they present improved bacterial resistance performance (living bacteria, 0%). This approach offers an antibacterial and anti-adhesive surface bearing small and well-defined quantities of in-situ generated AgNPs, and it is a novel, green, simple, and low-cost technique to generate AgNPs on soft biomedical substrates.

Emerging trends in clinical implications of bio-conjugated silver nanoparticles in drug delivery

“…Over the past decades, click chemistry reactions presented profound clinical applications of which the in-situ synthesis of enzyme inhibitors presents the most recent development [125], which also validates considerable biocompatibility of these reactions for designing bio-conjugated nanopharmaceuticals. However, the azide-alkyne click cycloaddition approach for developing bioconjugated AgNPs generally requires a tedious pre-synthesis of azide/ alkyne functionalized participating species [126]. In addition, this approach often suffers a low yield, thereby limiting its efficacy for nanoparticle bio-conjugation [127].…”

Sol–Gel Nanocomposite Coatings for Preventing Biofilm Formation on Contact Lens Cases

“… 28 To this end, silver is a well-studied material for creating antibacterial surfaces by a variety of methods, including physical deposition and chemical reduction; however, in most of these methods, silver particles are weakly bound to the surface and easily leach out in the solution, body fluids, and tissues. To circumvent this, Shen and associates 29 produced an anti-adhesive coating incorporating antibacterial material by covalently bonding specially designed silica microspheres with polydimethylsiloxane. Bare silver nanoparticles are spontaneously generated on the surface of these silica microspheres through the reduction process of silver ions by thiol groups.…”

“…The authors reported that such a surface treatment inhibited the growth of Escherichia coli and Bacillus subtilis . 29 However, the procedure reported is a multistep, time-consuming process; therefore, the quest to find an antibiofilm coating continues.…”

An Overview of Antimicrobial Properties of Carbon Nanotubes-Based Nanocomposites

“…155 Specifically, in the manufacturing of UTI devices, PDMS is usually applied due to its great biocompatibility, excellent chemical stability, and mechanical resistance. 156,157 However, the use of PDMS in the biomedical sector carries some drawbacks, such as it is vulnerable to non-specific surface attachment of pathogens and protein. Currently, various studies reported the enhancement of antibiofouling characteristics of PDMS by the attachment of MWCNTs.…”