Enhanced arsenic accumulation in Saccharomyces cerevisiae overexpressing transporters Fps1p or Hxt7p

Shah, Dhawal; Shen, Michael W. Y.; Chen, Wilfred; Silva, Nancy A. Da

doi:10.1016/j.jbiotec.2010.07.012

Cited by 34 publications

(22 citation statements)

References 35 publications

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“…These mechanisms reduce the cellular content of the toxic agent, although the molecular basis may differ among metals and organisms [19-21]. Differences in As sensitivities exist among plant accessions or varieties [11,22,23].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis

Chen

Nguyen

et al. 2014

BMC Plant Biol

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BackgroundArsenic (As) is a toxic metalloid found ubiquitously in the environment and widely considered an acute poison and carcinogen. However, the molecular mechanisms of the plant response to As and ensuing tolerance have not been extensively characterized. Here, we report on transcriptional changes with As treatment in two Arabidopsis accessions, Col-0 and Ws-2.ResultsThe root elongation rate was greater for Col-0 than Ws-2 with As exposure. Accumulation of As was lower in the more tolerant accession Col-0 than in Ws-2. We compared the effect of As exposure on genome-wide gene expression in the two accessions by comparative microarray assay. The genes related to heat response and oxidative stresses were common to both accessions, which indicates conserved As stress-associated responses for the two accessions. Most of the specific response genes encoded heat shock proteins, heat shock factors, ubiquitin and aquaporin transporters. Genes coding for ethylene-signalling components were enriched in As-tolerant Col-0 with As exposure. A tolerance-associated gene candidate encoding Leucine-Rich Repeat receptor-like kinase VIII (LRR-RLK VIII) was selected for functional characterization. Genetic loss-of-function analysis of the LRR-RLK VIII gene revealed altered As sensitivity and the metal accumulation in roots.ConclusionsThus, ethylene-related pathways, maintenance of protein structure and LRR-RLK VIII-mediated signalling may be important mechanisms for toxicity and tolerance to As in the species. Here, we provide a comprehensive survey of global transcriptional regulation for As and identify stress- and tolerance-associated genes responding to As.

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Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis

Chen

Nguyen

et al. 2014

BMC Plant Biol

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“…Therefore, genes related to As accumulation, transporter and hormone pathways may be As-tolerance associated (Fu et al 2014; Kumar et al 2015). In our study, genes encoding HXT and NRAMP were up-regulated and up-regulation of these genes can increase As accumulation (Shah et al 2010; Tiwari et al 2014), while genes encoding MT and GRX were down-regulated and up-regulation of these genes can lead to the decrease of As accumulation (Grispen et al 2009; Sundaram et al 2009). These results are good explanations to the ability of P. notoginseng to over-accumulate arsenic.…”

Section: Discussionmentioning

confidence: 68%

Illumina-based transcriptomic profiling of Panax notoginseng in response to arsenic stress

et al. 2016

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Background Panax notoginseng, a famous herbal medicine, has recently attracted great attention on its safety and quality since P. notoginseng can accumulate and tolerate As from growing environment. For the purpose of understanding As damage to the quality of P. notoginseng as well as corresponding tolerance mechanisms, genes involved in As stress response were identified using Illumina sequencing.ResultsTotally 91,979,946 clean reads were generated and were de novo assembled into 172,355 unigenes. A total of 81,575 unigenes were annotated in at least one database for their functions, accounting for 47.34 %. By comparative analysis, 1725 differentially expressed genes (DEGs, 763 up-regulated/962 down-regulated) were identified between As stressed plant (HAs) and control plant (CK), among which 20 DEGs were further validated by real-time quantitative PCR (qRT-PCR). In the upstream and downstream steps of biosynthesis pathways of ginsenosides and flavonoids, 7 genes encoding key enzymes were down-regulated in HAs. Such down-regulations were also revealed in pathway enrichment analysis. Genes encoding transporters (transporters of ABC, MATE, sugar, oligopeptide, nitrate), genes related to hormone metabolism (ethylene, ABA, cytokinin) and genes related to arsenic accumulation (HXT, NRAMP, MT and GRX) were differentially expressed. The up-regulated genes included those of oxidative stress-related protein (GSTs, thioredoxin), transcription factors (HSFs, MYBs) and molecular chaperones (HSP).ConclusionsThe down-regulation of biosynthesis of ginsenoside and flavonoid indicated that As accumulation in P. notoginseng can cause not only safety hazard, but also qualitative losses. Aside from the results of arsenic content of seedling roots, the ability of P. notoginseng to over-accumulate arsenic can also be explained by the differential expression of genes of HXT, NRAMP, MT and GRX. To illustrate the detoxification mechanism of P. notoginseng, differential expression of genes encoding oxidative-related proteins, transcription factors, molecular chaperones, transporters and hormone were revealed in our study, which agreed with those reported in Arabidopsis to a certain extent, indicating P. notoginseng and Arabidopsis shared some common detoxification mechanisms in response to As stress. The longer As treatment in our study may account for the smaller quantity of related DEGs and smaller degree of expression differences of certain DEGs compared with those of Arabidopsis.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-016-0128-8) contains supplementary material, which is available to authorized users.

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“…Although higher metal amounts can be removed from aqueous solutions (Avery and Tobin 1992;Mapolelo and Torto 2004;Ruta et al 2010;Shah et al 2010) or industrial effluents (Stoll and Duncan 1996) by live cells, the use of inactivated biomass shows several advantages: (a) the removal of metals is im-mune to the presence of toxics; (b) does not require an external energy source (nutrient supply); (c) yeast cells are easier stored as dried biomass; (d) biomass can be regenerated; (e) the process can operate in an analogous way to conventional ion-exchange technology; and (f) it is easier to recovery the metals from the biomass.…”

Section: Usefulness Of Using Dead Biomassmentioning

confidence: 99%

Cleanup of industrial effluents containing heavy metals: a new opportunity of valorising the biomass produced by brewing industry

Soares

2013

Appl Microbiol Biotechnol

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Heavy metal pollution is a matter of concern in industrialised countries. Contrary to organic pollutants, heavy metals are not metabolically degraded. This fact has two main consequences: its bioremediation requires another strategy and heavy metals can be indefinitely recycled. Yeast cells of Saccharomyces cerevisiae are produced at high amounts as a by-product of brewing industry constituting a cheap raw ma-terial. In the present work, the possibility of valorising this type of biomass in the bioremediation of real industrial efflu-ents containing heavy metals is reviewed. Given the auto-aggregation capacity (flocculation) of brewing yeast cells, a fast and off-cost yeast separation is achieved after the treat-ment of metal-laden effluent, which reduces the costs associ-ated with the process. This is a critical issue when we are looking for an effective, eco-friendly, and low-cost technolo-gy. The possibility of the bioremediation of industrial effluents linked with the selective recovery of metals, in a strategy of simultaneous minimisation of environmental hazard of indus-trial wastes with financial benefits from reselling or recycling the metals, is discussed.

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Enhanced arsenic accumulation in Saccharomyces cerevisiae overexpressing transporters Fps1p or Hxt7p

Cited by 34 publications

References 35 publications

Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis

Transcriptome profiling of genes and pathways associated with arsenic toxicity and tolerance in Arabidopsis

Illumina-based transcriptomic profiling of Panax notoginseng in response to arsenic stress

Cleanup of industrial effluents containing heavy metals: a new opportunity of valorising the biomass produced by brewing industry

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