Enhanced enzymatic hydrolysis of sugarcane bagasse hemicellulose using recombinant glucose oxidase expressed by Pichia pastoris

Yang, Gong; Zhang, Chuqiu; Yan, Qiuli; He, Wenjing; Xiao, Wenjuan; Lin, Jianghai; Liu, Zehuan

doi:10.1016/j.indcrop.2015.07.038

Cited by 11 publications

(2 citation statements)

References 32 publications

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“…To address these challenges, biotechnological advancements have been applied to enhance Gox production. A notable development is the heterologous expression of Gox genes, which has been achieved by modifying genes from Aspergillus niger for expression in Pichia pastoris, leading to significantly increased yields of Gox [43]. Additionally, research has shown that expressing the Gox gene in Yarrowia lipolytica using a hybrid promoter and secretion system substantially boosts Gox activity, particularly under controlled pH and temperature conditions [44].…”

Section: Addition Of Glucose Oxidasementioning

confidence: 99%

Low-Alcohol and Nonalcoholic Wines: From Production to Cardiovascular Health, along with Their Economic Effects

Silva

2024

Beverages

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This review aims to create a communication tool for low-alcohol and nonalcoholic wine production, catering to scientists, educators, students, and wine producers in the field. With health concerns regarding alcohol consumption, the need for information on low-alcohol wines is essential. This paper outlines the methods for the pre-fermentation (leaf area reduction, early grape harvest, grape must dilution, filtration of grape juice and addition of glucose oxidase), mid-fermentation (employing non-saccharomyces yeasts, using genetically modified yeasts through metabolic engineering, and controlling yeast nutrition), and post-fermentation (nanofiltration and reverse osmosis, osmotic distillation, pervaporation, spinning cone column, vacuum distillation, and multi-stage membrane-based systems) stages and their effects on wine quality. It also presents evidence of the impact of alcoholic, low-alcohol, and nonalcoholic wines on cardiovascular health. Finally, the potential market for low-alcohol and nonalcoholic wines is discussed. Key findings indicate a shift toward low-alcohol alternatives due to health, economic, and social factors and consumer interest in healthier lifestyles. Low-alcohol and nonalcoholic wines offer health benefits, particularly cardiovascular health, presenting an opportunity for winemakers to cater to a health-conscious market. From an economic perspective, the low-alcohol and nonalcoholic wine market is poised to grow and diversify its revenue streams. The development of high-quality low-alcohol and nonalcoholic wines, which can command premium prices, enhances profitability. The changing regulatory landscape in Europe, with a focus on transparency in alcohol labeling and nutritional information, aligns with the new consumer preferences and regulatory standards.

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Section: Addition Of Glucose Oxidasementioning

confidence: 99%

Low-Alcohol and Nonalcoholic Wines: From Production to Cardiovascular Health, along with Their Economic Effects

Silva

2024

Beverages

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“…An attractive alternative to microbial fuel cell-driven EF processes is the direct production of electrons through the glucose oxidation process. Glucose fuel cells can be classied into three types according to the nature of the catalyst: (i) enzymatic catalyst, 18 (ii) microbial catalyst 19 and (iii) metal catalyst. 20 The electro-oxidation of non-enzymatic glucose with metallic nanoparticles displays long-term stability and can be performed on electrode materials containing Ni, 21 Pt, 22 Pd-Rh, 23 Pt-Ru, 24 Au 24 or Au-Pt-Pd.…”

Section: Introductionmentioning

confidence: 99%

Design of a novel fuel cell-Fenton system: a smart approach to zero energy depollution

Esmilaire

Drobek

et al. 2016

J. Mater. Chem. A

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Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion

Zhou,

Zhang,

Qian

2023

Biotech & Bioengineering

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Glucose oxidase (EC 1.1.3.4, GOD) is a widely used industrial enzyme. To construct a GOD‐hyperproducing Pichia pastoris strain, combinatorial strategies have been applied to improve GOD activity, synthesis, and secretion. First, wild‐type GOD was subjected to saturation mutagenesis to obtain an improved variant, MGOD1 (V20W/T30S), with 1.7‐fold higher kcat/KM. Subsequently, efficient signal peptides were screened, and the copy number of MGOD1 was optimized to generate a high‐producing strain, 8GM1, containing eight copies of AOX1 promoter‐GAS1 signal peptide‐MGOD1 expression cassette. Finally, the vesicle trafficking of 8GM1 was engineered to obtain the hyperproducing strain G1EeSe co‐expressing the trafficking components EES and SEC. 22, and the EES gene (PAS_chr3_0685) was found to facilitate both protein secretion and production for the first time. Using these strategies, GOD secretion was enhanced 65.2‐fold. In the 5‐L bioreactor, conventional fed‐batch fermentation without any process optimization resulted in up to 7223.0 U/mL extracellular GOD activity (3.3‐fold higher than the highest level reported to date), with almost only GOD in the fermentation supernatant at a protein concentration of 30.7 g/L. Therefore, a GOD hyperproducing strain for industrial applications was developed, and this successful case can provide a valuable reference for the construction of high‐producing strains for other industrial enzymes.

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Enhanced enzymatic hydrolysis of sugarcane bagasse hemicellulose using recombinant glucose oxidase expressed by Pichia pastoris

Cited by 11 publications

References 32 publications

Low-Alcohol and Nonalcoholic Wines: From Production to Cardiovascular Health, along with Their Economic Effects

Low-Alcohol and Nonalcoholic Wines: From Production to Cardiovascular Health, along with Their Economic Effects

Design of a novel fuel cell-Fenton system: a smart approach to zero energy depollution

Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion

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