Enhanced fitness of a <i><scp>B</scp>actrocera cucurbitae</i> genetic sexing strain based on the addition of gut‐isolated probiotics (<i><scp>E</scp>nterobacter</i> spec.) to the larval diet

Yao, Mingyan; Zhang, Hehe; Cai, Pumo; Gu, Xiaohong; Wang, Dan; Ji, Qinge

doi:10.1111/eea.12529

Cited by 30 publications

(40 citation statements)

References 34 publications

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“…Irradiation has been reported to significantly destabilize fly fitness by damaging tissues and interior organs to some degree, inducing metabolic disorders (Lauzon and Potter, 2012;Yao et al, 2017), and our results showed that irradiation significantly altered the transcript levels of OBP genes at all adult life stages ( Figure 5a, 5b and 5c), with especially abundant expression in the second and third stages (Figure 5b, 5c). These significant observations suggest that the effects of applied treatments on the transcript levels of BdorOBP genes may play an essential role in understanding the insect olfaction mechanism in response to different kinds of Idrees et Our results provide a direct BdorOBP transcript analysis that represents new evidence for the role of olfactory proteins in the reception of protein baits and volatiles.…”

Section: Discussionsupporting

confidence: 62%

Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

Idrees¹

2017

Appl. Ecol. Env. Res.

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Idrees et al.: Protein baits, volatile compounds and irradiation influence the expression profiles of odorant-binding protein genes inBactrocera dorsalis Fuzhou, Fujian, 350002, *Corresponding author e-mail: jiqinge@yeah.net; phone: +86-0-59-183-789-420; fax:+86-0-59-183-789-421 (Received 4 th Jul 2017; accepted 18 th Oct 2017)Abstract. Chemical communication in insects is based on odorant-binding proteins (OBPs), but the molecular mechanisms by which these OBPs allow the perception of volatiles and host chemicals remain unclear. Bactrocera dorsalis (Hendel) is one of the most economically important pests of fruits and vegetables. In this study, the OBPs of B. dorsalis (BdorOBPs) were evaluated in response to different attractive protein baits, brewer's yeast volatile compounds and irradiation. The expression of 10 genes encoding OBPs in the antennae of B. dorsalis during three adult physiological life stages (pre-mating, post-mating and post-oviposition) were analysed. All selected BdorOBP genes were found to contain one conserved pheromone-binding protein/general-odorant-binding protein domain (PBP-GOBP), except OBP8, which contained two of these conserved domains. An unrooted phylogenetic tree was constructed to show the relationships among these 10 BdorOBPs and the OBPs belonging to the same OBP family in other insects. We found significantly different transcript expression profiles in each OBP gene at each stage in response to different treatments; these results revealed that OBP2 expression was significantly increased in response to baits at each adult physiological stage, while OBP2, OBP5 and OBP1 were highly expressed in response to combined-volatiles treatment at all tested physiological stages. OBP3, OBP5 and OBP10 showed high expression in response to irradiation at all tested physiological stages. Thus, we infer that protein baits, brewer's yeast volatiles and irradiation significantly influence the transcript levels of OBP genes, which may act in olfactory perception.

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Section: Discussionsupporting

confidence: 62%

Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

Idrees¹

2017

Appl. Ecol. Env. Res.

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“…Colonization, mass-rearing, irradiation, transport, and release (usually by air) may affect the quality of the insects used in AW-IPM programs with an SIT component (Collins et al, 2008;Kumano et al, 2008). Symbiotic microbial communities harbored in fruit flies can be harnessed for probiotic applications (Ben Ami et al, 2009;Augustinos et al, 2015;Kyritsis et al, 2017;Yao et al, 2017). In addition, studies of insect microbiota and the alterations induced upon irradiation could be informative and exploited in order to produce higher quality sterile individuals for SIT applications at a reduced cost, by supplementing those bacterial taxa that are highly affected during the irradiation process.…”

Section: Introductionmentioning

confidence: 99%

Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Stathopoulou

Asimakis

Khan

et al. 2019

Entomologia Exp Applicata

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The role of symbiotic microbes in insects, especially the beneficial character of this interaction for insects, has received much attention in recent years as it has been related to important aspects of the host insects' biology such as development, reproduction, survival, and fitness. Among insect hosts, tephritid fruit flies are well known to form beneficial associations with their symbionts. To control these destructive agricultural pests, environmentally friendly approaches, like the sterile insect technique as a component of integrated pest management strategies, remain most effective. In this study, changes in the bacterial profile of mass‐reared oriental fruit flies, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), were examined in both larval and adult stages and also after irradiation by employing a 16S rRNA gene‐based Illumina sequencing approach. Proteobacteria was the prevalent bacterial phylum in non‐irradiated adults and larvae. Alphaproteobacteria was the most abundant class in larvae but almost absent in adults, which was dominated by Gammaproteobacteria. Firmicutes were present in both developmental stages but at lower relative abundance. At genus level, Acetobacter prevailed in the larval stage and members of the Enterobacteriaceae family in adults. Irradiated samples exhibited higher diversity and richness indices compared to the non‐irradiated oriental fruit flies, whereas no significant changes were observed between the two developmental stages of the non‐irradiated samples. Lactobacillus, members of the Orbacecae family, and Morganella were detected but to a lesser degree upon irradiation, whereas the relative abundance of Lactococcus and Orbus increased. The bacterial profile of larvae appeared to be different compared to that of adult B. dorsalis flies. The subsequent application of irradiation at the pupal stage led to the development of different microbiota between treated and untreated samples, affecting diversity and operational taxonomic unit composition. Irradiated samples of oriental fruit flies were characterized by higher species diversity and richness.

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“…Yao et al. () discovered that adding live as well as dead Enterobacter isolates from the medfly to the larval diet enhanced the fitness of a Bactrocera cucurbitae (Coquillett) genetic sexing strain by increasing pupal weight and survival rate.…”

Section: Potential Of Gut Symbionts For Olive Fruit Fly Rearingmentioning

confidence: 99%

Review of the role of gut microbiota in mass rearing of the olive fruit fly,Bactrocera oleae, and its parasitoids

Ras

Beukeboom

Cáceres

et al. 2017

Entomologia Exp Applicata

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The olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), is the major insect pest in commercial olive (Olea europaea L., Oleaceae) production worldwide. Its population management is largely based on the use of insecticides. However, concerns about the impact of insecticides on the environment and human health along with increasing resistance development calls for novel and environment-friendly approaches for population management. Integrated pest management programmes with a sterile insect technique (SIT) component and parasitoids are currently considered for the control of B. oleae. A major challenge for the development of such tools is mass rearing of both host and parasitoids. In this review, we consider the role of endogenous microbiota and its potential exploitation for improving the efficacy, quality, and cost effectiveness of mass rearing B. oleae as well as their parasitoids.

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Enhanced fitness of a Bactrocera cucurbitae genetic sexing strain based on the addition of gut‐isolated probiotics (Enterobacter spec.) to the larval diet

Cited by 30 publications

References 34 publications

Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis

Review of the role of gut microbiota in mass rearing of the olive fruit fly,Bactrocera oleae, and its parasitoids

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