Enhanced Introduction of Gold Nanoparticles into Vital <i>Acidothiobacillus ferrooxidans</i> by Carbon Nanotube-based Microwave Electroporation

Rojas-Chapana, José; Correa‐Duarte, Miguel A.; Z, Ren; Kempa, Krzysztof; Giersig, Michael

doi:10.1021/nl049699n

Cited by 115 publications

(79 citation statements)

References 14 publications

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“…[78] The bacterial suspensions were prepared by the dispersion of pellet cells from a culture in a colloidal gold solution containing hydrophilic CNTs. The filtered bacterial suspension was centrifuged and the supernatant discarded.…”

Section: Wwwchemeurjorgmentioning

confidence: 99%

Carbon Nanotube and Gold‐Based Materials: A Symbiosis

Singh

Premkumar

Shin

et al. 2010

Chemistry A European J

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Carbon nanotubes constitute a novel class of nanomaterials with potential applications in many areas. The attachment of metal nanoparticles to carbon nanotubes is new way to obtain novel hybrid materials with interesting properties for various applications such as catalysts and gas sensors as well as electronic and magnetic devices. Their unique properties such as excellent electronic properties, a good chemical stability, and a large surface area make carbon nanotubes very useful as a support for gold nanoparticles in many potential applications, ranging from advanced catalytic systems through very sensitive electrochemical sensors and biosensors to highly efficient fuel cells. Here we give an overview on the recent progress in this area by exploring the various synthesis approaches and types of assemblies, in which nanotubes can be decorated with gold nanoparticles and explore the diverse applications of the resulting composites.

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Section: Wwwchemeurjorgmentioning

confidence: 99%

Carbon Nanotube and Gold‐Based Materials: A Symbiosis

Singh

Premkumar

Shin

et al. 2010

Chemistry A European J

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“…It is clear, that while the CNT with the aspect ratio 5 enhances the field at the tip by a factor of 15, the tube with the aspect ratio 10 more than doubles that value to 35. Even though these calculations are static, they apply to the microwave fields as well, because the plasma frequencies of both CNTs and gold nanoparticles are much higher (UV range) than the microwave frequencies [12]. Since dipoles both on the surface and interstitial channels of nanotubes are involved in the activation of single wall CNTs [9], the interfacial mechanism for CNT-cell targeting was investigated.…”

Section: Discussionmentioning

confidence: 99%

“…MW activated CNTs operating through the so-called "end tip" mode of action would have a greater potential for membrane discrimination, if the end tips are functionalised, thus creating molecular probes (resistant to proteasas) with potential applications in cell biology and biomedicine [12,13]. Further, this discovery allows us to explore their potential application as nanodevices for electroporation.…”

Section: Discussionmentioning

confidence: 99%

Novel electroporation System for both Gram-negative and Gram-positive Bacteria Assisted by Multi-Walled Carbon Nanotubes

et al. 2004

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Gram positive (Lactoccocus lactis) and Gram negative bacteria (Escherichia coli)were used to study the interaction of water-dispersible muti-walled carbon nanotubes (CNTs) with the bacterial cell envelope during microwave (MW) energy exposure. It was observed that the addition of a tiny amount of CNTs to a medium containing bacteria and subsequent exposure of the samples to MW, leads to an intimate contact between the CNT tips and the cell envelope. This phenomenon can be explained in terms of attractive forces between opposite charges of polar structures. Since CNTs under MW irradiation behave like electric dipoles, this would make it possible for the CNTs to target the cell surface without inducing changes in the cell shape and viability. Thus, the electrochemical properties of CNTs and their capillarity make them useful tools for cell manipulation, and therefore for the intracellular transport of drugs, dyes or biomolecules.

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“…Some improved approaches, including disruption of endosomes, direct microinjection, electroporation, and conjugation of natural cell-penetrating/fusogenic chaperones to nanomaterials, have been utilized to enhance nanoparticle endocytic uptake with minimal toxicity [17][18][19]. Among these novel approaches, ultrasound (US) in combination with microbubbles (MBs) has received increasing interest as an activation mechanism for carrier delivery because of its advantages in spatial and temporal control of compound release [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Altering the response of intracellular reactive oxygen to magnetic nanoparticles using ultrasound and microbubbles

Yang

Cui

et al. 2015

Sci. China Mater.

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Engineered iron oxide magnetic nanoparticles (MNPs) are one of the most promising tools in nanomedicine-based diagnostics and therapy. However, increasing evidence suggests that their specific delivery efficiency and potential long-term cytotoxicity remain a great concern. In this study, using 12 nm γ-Fe2O3 MNPs, we investigated three types of uptake pathways for MNPs into HepG2 cells: (1) a conventional incubation endocytic pathway; (2) MNPs co-administrated with microbubbles under ultrasound exposure; and (3) ultrasound delivery of MNPs covalently coated on the surface of microbubbles. The delivery efficiency and intracellular distribution of MNPs were evaluated, and the cytotoxicity induced by reactive oxygen species (ROS) was studied in detail. The results show that MNPs can be delivered into the lysosomes via classical incubation endocytic internalization; however, microbubbles and ultrasound allow the MNPs to pass through the cell membrane and enter the cytosol via a non-internalizing uptake route much more evenly and efficiently. Further, these different delivery routes result in different ROS levels and antioxidant capacities, as well as intracellular glutathione peroxidase activity for HepG2 cells. Our data indicate that the microbubble-ultrasound treatment method can serve as an efficient cytosolic delivery strategy to minimize long-term cytotoxicity of MNPs.

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Enhanced Introduction of Gold Nanoparticles into Vital Acidothiobacillus ferrooxidans by Carbon Nanotube-based Microwave Electroporation

Cited by 115 publications

References 14 publications

Carbon Nanotube and Gold‐Based Materials: A Symbiosis

Carbon Nanotube and Gold‐Based Materials: A Symbiosis

Novel electroporation System for both Gram-negative and Gram-positive Bacteria Assisted by Multi-Walled Carbon Nanotubes

Altering the response of intracellular reactive oxygen to magnetic nanoparticles using ultrasound and microbubbles

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