Enhanced methanogenic co-degradation of propionate and butyrate by anaerobic microbiome enriched on conductive carbon fibers

Barua, Sajib; Zakaria, Basem S.; Dhar, Bipro Ranjan

doi:10.1016/j.biortech.2018.06.053

Cited by 86 publications

(25 citation statements)

References 49 publications

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“…Studies have indicated that Anaerolineaceae species are keystone microbes that participate in degrading organic matter (In’t Zandt et al, 2018; Meng et al, 2019). Previously, Methanosaeta species have been believed to be limited to acetate, which is a substrate for methane production, living in wastewater, marshes and wetlands and releasing methane and carbon dioxide by breaking down the acetic salts produced by other microbes in the environment (Barua et al, 2018; Ji et al, 2018). In addition, as the dominant bacteria in the aggregates, Geobacter species were highly connected with NO 3 − -N and expressed genes related to ethanol metabolism, suggesting that Geobacter and Methanosaeta species exchange electrons through direct interspecific electron transfer (Rotaru et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Microbial Community Structures and Important Associations Between Soil Nutrients and the Responses of Specific Taxa to Rice-Frog Cultivation

Yi²,

Fang

et al. 2019

Front. Microbiol.

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Rice-frog cultivation is a traditional farming system in China and has been reintroduced as an agricultural practice in China in recent years. The microbial community in paddy rhizospheric soils has attracted much attention because many microorganisms participate in functional processes in soils. In this study, Illumina MiSeq sequencing-based techniques were used to investigate soil microbial communities and functional gene patterns across samples obtained by conventional rice cultivation (CR) and rice-frog cultivation (RF). The results showed that RF significantly affected the microbial community composition and richness, which indicated that the rhizospheric microorganisms responded to the introduction of tiger frogs into the paddy fields. Operational taxonomic units (OTUs) from Sandaracinaceae , Anaerolineaceae , Candidatus Nitrososphaera, Candidatus Nitrosotalea, Candidatus Nitrosoarchaeum and some unclassified OTUs from Euryarchaeota and Agaricomycetes were significantly enriched by RF. The abiotic parameters soil organic carbon (SOC), nitrate nitrogen (NO 3 − -N), and available phosphorus (AP) changed under RF treatment and played essential roles in establishing the soil bacterial, archaeal, and fungal compositions. Correlations between environmental factors and microbial communities were described using network analysis. SOC was strongly correlated with Anaerolineaceae , Methanosaeta , and Scutellinia . NO 3 − -N showed strong positive correlations with Opitutus , Geobacter , and Methanosaeta. NH 4 + -N was strongly positively associated with Sideroxydans , and TN was strongly positively correlated with Candidatus Nitrotoga. Compared to conventional CR, RF greatly enriched specific microbial taxa. These taxa may be involved in the decomposition of complex organic matter and the transformation of soil nutrients, thus promoting plant growth by improving nutrient cycling. The unique patterns of microbial taxonomic and functional composition in soil profiles suggested functional redundancy in these paddy soils. RF could significantly affect the bacterial, archaeal, and fungal communities though changing SOC and AP levels.

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Section: Discussionmentioning

confidence: 99%

Microbial Community Structures and Important Associations Between Soil Nutrients and the Responses of Specific Taxa to Rice-Frog Cultivation

Yi²,

Fang

et al. 2019

Front. Microbiol.

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“…Importantly, in the presence of CMs, DIET could be stimulated without the need for electrically conductive pili (e-pili) and outer-surface c-type cytochromes [2]. Various CM, e.g., magnetite (Fe 3 O 4 ) [23], carbon cloth [24], granular activated carbon (GAC) [25], biochar [26], ferro/ferric oxide [27], and carbon fiber [28] were supplemented to AD systems and brought about improved CH 4 production rates and yields. Based on their electrical conductivity properties and ability for long-range electron exchange, CMs could work as electron conduits between syntrophic and methanogenic consortia [29,30].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Anaerobic Digestion of Long Chain Fatty Acid by Adding Magnetite and Carbon Nanotubes

Mostafa

Song

et al. 2020

Microorganisms

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This study investigated the impact of stimulating direct interspecies electron transfer (DIET), by supplementing nano-sized magnetite (nFe3O4, 0.5 g Fe/g VSS) and carbon nanotubes (CNT, 1 g/L), in anaerobic digestion of oleic acid (OA) at various concentrations (0.10–4.00 g chemical oxygen demand(COD)/L). Both supplementations could enhance CH4 production, and its beneficial impact increased with increased OA concentration. The biggest improvements of 114% and 165% compared to the control were achieved by nFe3O4 and CNT, respectively, at OA of 4 g COD/L. The enhancement can be attributed to the increased sludge conductivity: 7.1 ± 0.5 (control), 12.5 ± 0.8 (nFe3O4-added), and 15.7 ± 1.1 µS/cm (CNT-supplemented). Dissolved iron concentration, released from nFe3O4, seemed to have a negligible role in improving CH4 production. The excretion of electron shuttles, i.e., humic-like substances and protein-like substances, were found to be stimulated by supplementing nFe3O4 and CNT. Microbial diversity was found to be simplified under DIET-stimulating conditions, whereby five genera accounted for 88% of the total sequences in the control, while more than 82% were represented by only two genera (Methanotrix concilli and Methanosarcina flavescens) by supplementing nFe3O4 and CNT. In addition, the abudance of electro-active bacteria such as Syntrophomonas zehnderi was significantly increased from 17% to around 45%.

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“…However, in this study, much higher acetate concentration (up to 1,000 mg/L) than that of propionate (<200 mg/L) and butyrate (<100 mg/L) was observed in the last stage (see Figure 5), which indicated acetoclastic methanogenesis could be the rate‐limiting step at high OLR of 3.01 g COD/L‐d. Previous studies also demonstrated that the amendment of conductive materials could accelerate the syntrophic propionate or butyrate degradation by facilitating DIET (Barua, Zakaria, & Dhar, 2018; Zhao et al., 2016). Nonetheless, as the pH (7.2) in this stage was still in the optimum range (6.8–7.2) (Cioabla, Ionel, Dumitrel, & Popescu, 2012), stable performance was sustained without system failure and instability.…”

Section: Resultsmentioning

confidence: 96%

“…However, in this study, high OLR of 3.01 g COD/L‐d was achieved with a stable performance at 20℃. It has been suggested that conductive carbon fibers could promote the DIET between exoelectrogens and electroactive methanogens (Barua et al., 2018, 2019). In this study, the deployment of carbon fibers as the biofilm support media could maintain the process stability at high OLRs.…”

Section: Resultsmentioning

confidence: 99%

A high‐rate anaerobic biofilm reactor for biomethane recovery from source‐separated blackwater at ambient temperature

Huang

Zakaria

Zhang

et al. 2020

Water Environment Research

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Anaerobic bioreactors for source-separated blackwater are mostly operated at low organic loading rates (OLRs) due to low biodegradability and the potential of ammonia inhibition. In this study, an anaerobic biofilm reactor having conductive carbon fibers as the media was investigated for the high-rate treatment of blackwater collected from vacuum toilets. The bioreactor was operated at different OLRs ranged from 0.77 to 3.01 g COD/L-d in four stages for a total operating period of ~ 250 days. With the increase of OLRs, the specific methane production rate increased from 105.3 to 304.6 ml/L-d with high methane content in biogas (75.5%-83%). The maximum methane yield was achieved at hydraulic retention time (HRT) of 15 days. Highest organics and suspended solids removal (80%-83%) were achieved at 20-days HRT, while increased OLRs resulted in diminished removal efficiencies. The state variables, including pH, total ammonia nitrogen, short-chain volatile fatty acids, and soluble chemical oxygen demand, indicated the system had a great capability to withstand the high OLRs. Microbial community analysis revealed that the high performance might be attributed to direct interspecies electron transfer (DIET) facilitated by potentially electroactive bacteria (e.g., Syntrophomonas, Clostridium) and electrotrophic archaea (e.g., Methanosaeta and Methanosarcina species) enriched on the carbon fibers. © 2020 Water Environment Federation • Practitioner points • An anaerobic biofilm reactor was investigated for biomethane recovery from sourceseparated blackwater. • Conductive carbon fibers were utilized as the media to stimulate enrichment of potentially electroactive methanogenic communities. • The bioreactor was operated at ambient temperature for over 250 days. • High methane production rate and high-quality biogas were achieved at OLRs ranged from 0.77 to 3.01 g COD/L-d. • Microbial community analysis suggested direct interspecies electron transfer (DIET) between specific electroactive bacteria and electrotrophic archaea.

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Enhanced methanogenic co-degradation of propionate and butyrate by anaerobic microbiome enriched on conductive carbon fibers

Cited by 86 publications

References 49 publications

Microbial Community Structures and Important Associations Between Soil Nutrients and the Responses of Specific Taxa to Rice-Frog Cultivation

Microbial Community Structures and Important Associations Between Soil Nutrients and the Responses of Specific Taxa to Rice-Frog Cultivation

Enhanced Anaerobic Digestion of Long Chain Fatty Acid by Adding Magnetite and Carbon Nanotubes

A high‐rate anaerobic biofilm reactor for biomethane recovery from source‐separated blackwater at ambient temperature

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