Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems

Posada, Luisa F.; Álvarez, Javier Correa; Romero-Tabarez, Magally; de‐Bashan, Luz E.; Villegas-Escobar, Valeska

doi:10.1016/j.micres.2018.08.017

Cited by 43 publications

(36 citation statements)

References 47 publications

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“…Also, the inoculation with A. brasilense provided an increase of increase of 10.% for biomass P uptake (40.37 to 44.42 kg ha −1 ) and 21.3% for grain P uptake (49.87 to 60.50 kg ha −1 , respectively) compared to control, regardless of P 2 O 5 application rates. The exact mechanisms underlying the B. subtilis and A. brasilense effect on corn growth was not evaluated in the present study, however, it is very likely that the improvement in P availability and uptake reflecting on improved PUE and grain yield by corn verified in this study is associated with the well-known ability of A. brasilense and B. subtilis to promote plant growth (Fukami et al, 2017;Jang et al, 2018;Martins et al, 2018;Posada et al, 2018;Salvo et al, 2018). The increase on PUE and corn yield as a function B. subtilis and A. brasilense inoculation compared to control was, on average, equivalent to 100.5 and 54.6% for PUE and 17.4 and 12.8% for grain yield, respectively, regardless of P 2 O 5 application rates.…”

Section: Discussionmentioning

confidence: 87%

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

Pereira

Galindo

Gazola

et al. 2020

Front. Environ. Sci.

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The use of plant growth promoting bacteria (PGPB) that can solubilize phosphorus (P) has shown potential to improve nutrient availability in grass crops such as corn (Zea mays L.) This study was developed to investigate if inoculation with Azospirillum brasilense, Bacillus subtilis or Pseudomonas fluorescens associated with P 2 O 5 rates can improve phosphorus use efficiency (PUE) reflecting on greater corn development and yield. The field trial was set up in a Rhodic Hapludox under no-till system under Savannah conditions, in a completely randomized block design with four replicates. Treatments were tested in a full factorial design and included: (i) five P 2 O 5 rates (0 to 105 kg ha −1) and (ii) four PGPB seed inoculation (Control-without inoculation, A. brasilense, B. subtilis or P. fluorescens). Inoculation was found to increase grain yield by 39.5, 29.1, and 15.9% when B. subtilis was inoculated in the absence of P 2 O 5 rates and associated with 17.5 and 70 kg P 2 O 5 ha −1 and by 34.7% when A. brasilense was inoculated with application of 105 kg P 2 O 5 ha −1. In addition, inoculation with B. subtilis and A. brasilense were found to increase P uptake, benefiting productive components development, leading to an improved PUE, and greater corn grain yield. The results of this study showed positive improvements in P uptake as a result of B. subtilis and A. brasilense inoculation, with an increase of 100.5 and 54.6% on PUE, respectively; while the P. fluorescens inoculation were less evident. Further research should be conducted under biotic or/and abiotic conditions such as attack of pathogens and insects, drought, salinity, water flooding, low and high temperature to better understand the role of PGPB, inoculated alone or in combination as the co-inoculated method.

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Section: Discussionmentioning

confidence: 87%

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

Pereira

Galindo

Gazola

et al. 2020

Front. Environ. Sci.

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“…The biofilm has advantages to bacterial cells because it gives them protection from predators, provides a physical barrier against the diffusion of unwanted molecules and helps them retain nutrients ( Bogino et al, 2013 ). Similar types of biofilm matrix were formed by Bacillus subtilis EA-CB0575 in tomato and banana roots ( Posada et al, 2018 ).…”

Section: Discussionmentioning

confidence: 91%

“…A mesh-like form adhered to inoculated Brachypodium roots before and after root exudate collection. Hence, rinsing the roots in ultrapure water did not affect the biofilm formation on inoculated roots and is required for cell fixation and colonization of plant tissues ( Gagné-Bourque et al, 2015 ; Posada et al, 2018 ). This matrix was absent in control roots.…”

Section: Discussionmentioning

confidence: 99%

A Crosstalk Between Brachypodium Root Exudates, Organic Acids, and Bacillus velezensis B26, a Growth Promoting Bacterium

et al. 2020

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Plant growth-promoting rhizobacteria (PGPR) are associated with plant roots and use organic compounds that are secreted from root exudates as food and energy source. Root exudates can chemoattract and help bacteria to colonize the surface of plant roots by inducing chemotactic responses of rhizospheric bacteria. In this study, we show that root colonization of Brachypodium distachyon by Bacillus velezensis strain B26 depends on several factors. These include root exudates, organic acids, and their biosynthetic genes, chemotaxis, biofilm formation and the induction of biofilm encoding genes. Analysis of root exudates by GC-MS identified five intermediates of the TCA cycle; malic, fumaric, citric, succinic, oxaloacetic acids, and were subsequently evaluated. The strongest chemotactic responses were induced by malic, succinic, citric, and fumaric acids. In comparison, the biofilm formation was induced by all organic acids with maximal induction by citric acid. Relative to the control, the individual organic acids, succinic and citric acids activated the epsD gene related to EPS biofilm, and also the genes encoding membrane protein ( yqXM ) and hydrophobin component ( bslA ) of the biofilm of strain B26. Whereas epsA and epsB genes were highly induced genes by succinic acid. Similarly, concentrated exudates released from inoculated roots after 48 h post-inoculation also induced all biofilm-associated genes. The addition of strain B26 to wild type and to icdh mutant line led to a slight induction but not biologically significant relative to their respective controls. Thus, B26 has no effect on the expression of the ICDH gene, both in the wild type and the mutant backgrounds. Our results indicate that root exudates and individual organic acids play an important role in selective recruitment and colonization of PGPR and inducing biofilm. The current study increases the understanding of molecular mechanisms behind biofilm induction by organic acids.

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“…Las biopelículas pueden ser consideradas como capas compactas relativamente uniformes, formadas por el crecimiento concertado y organizado de microorganismos. La formación de estas biopelículas les permite a los agentes de control biológico ser buenos colonizadores (Posada et al, 2018), ya que les proporciona una ventaja adaptativa al proteger a la población de condiciones ambientales adversas y otros factores mecánicos, lo que puede conducir además a la exclusión de nicho de los patógenos (Lalloo et al, 2010;Rafique et al, 2015).…”

Section: Competencia Y Colonizaciónunclassified

Mecanismos de acción de <i>Bacillus</i> spp. (Bacillaceae) contra microorganismos fitopatógenos durante su interacción con plantas

2020

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Algunos Bacillus spp. promotores de crecimiento vegetal son microorganismos reconocidos como agentes de control biológico que forman una estructura de resistencia denominada endospora, que les permite sobrevivir en ambientes hostiles y estar en casi todos los agroecosistemas. Estos microorganismos han sido reportados como alternativa al uso de agroquímicos. Sus mecanismos de acción se pueden dividir en: producción de compuestos antimicrobianos, como son péptidos de síntesis no ribosomal (NRPs) y policétidos (PKs); producción de hormonas, capacidad de colonización, formación de biopelículas y competencia por espacio y nutrientes; síntesis de enzimas líticas como quitinasas, glucanasas, protesasas y acil homoserin lactonasas (AHSL); producción de compuestos orgánicos volátiles (VOCs); e inducción de resistencia sistémica (ISR). Estos mecanismos han sido reportados en la literatura en diversos estudios, principalmente llevados a cabo a nivel in vitro. Sin embargo, son pocos los estudios que contemplan la interacción dentro del sistema tritrófico: planta – microorganismos patógenos – Bacillus sp. (agente biocontrolador), a nivel in vivo. Es importante destacar que la actividad biocontroladora de los Bacillus es diferente cuando se estudia bajo condiciones de laboratorio, las cuales están sesgadas para lograr la máxima expresión de los mecanismos de acción. Por otra parte, a nivel in vivo, la interacción con la planta y el patógeno juegan un papel fundamental en la expresión de dichos mecanismos de acción, siendo esta más cercana a la situación real de campo. Esta revisión se centra en los mecanismos de acción de los Bacillus promotores de crecimiento vegetal, expresados bajo la interacción con la planta y el patógeno.

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Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems

Cited by 43 publications

References 47 publications

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

A Crosstalk Between Brachypodium Root Exudates, Organic Acids, and Bacillus velezensis B26, a Growth Promoting Bacterium

Mecanismos de acción de <i>Bacillus</i> spp. (Bacillaceae) contra microorganismos fitopatógenos durante su interacción con plantas

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